ABSTRACT
Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated that only 27 (21 percent) samples were positive for E. histolytica/E. dispar complex. Among these stool samples, 11 were positive by Multiplex-PCR, with nine presenting the diagnostic fragment characteristic of E. dispar (96 bp) and two presenting diagnostic fragment of E. histolytica (132 bp). Among negative samples detected by microscopic examination, three positive samples for E. dispar and one positive for E. histolytica by Multiplex-PCR was observed. This denotes a low sensibility of microscopic examination when a single stool sample is analyzed. Assay for detection of E. histolytica antigen was concordant with multiplex-PCR in relation to E. histolytica. Statistical analysis comparing the sensibility tests was not done because of the low number of E. histolytica cases. The results demonstrate the importance of the specific techniques use for the differentiation between E. histolytica and E. dispar.
Subject(s)
Animals , Humans , DNA, Protozoan/analysis , Entamoeba histolytica/genetics , Entamoebiasis/diagnosis , Feces/parasitology , Polymerase Chain Reaction/methods , Antigens, Protozoan/analysis , Diagnosis, Differential , DNA, Protozoan/genetics , Entamoeba histolytica/immunology , Entamoeba histolytica/isolation & purification , Entamoeba/genetics , Entamoeba/immunology , Entamoeba/isolation & purification , Entamoebiasis/parasitology , Immunoenzyme Techniques , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
São descritos dois casos de fasciolíase em áreas rurais do Rio de Janeiro, endêmicas para esquistossomose, sendo ambos surpreendidos durante inquéritos coprológicos. O paciente de Paracambi queixava-se de tonteira. A paciente de Sumidouro queixava-se de tonteira, cansaço e tosse, tendo sido tratada com praziquantel; seus exames de controle foram negativos.
Subject(s)
Humans , Animals , Male , Female , Adult , Middle Aged , Fascioliasis/diagnosis , Anthelmintics/therapeutic use , Brazil , Fascioliasis/drug therapy , Praziquantel/therapeutic use , Rural PopulationABSTRACT
O enfrentamento de doenças transmissíveis de origem socioambiental esbarra frequentemente em dificuldades de etendimento entre comunidades, poder público e cientistas, ainda que haja recursos técnicos abundantes e adequados. Esse trabalho descreve uma experiência de reflexaão sobre dificuldades encontradas pelos integrantes de um grupo interdisciplinar e multissetorial para esse enfrentamento e sugre uma metodologia baseasda em diálogo para prevenção de impasses entre setores, disciplinas e sujeitos. A metoldologia consiste em um processo, ainda em curso, baseado na consolidação de um grupo multissetorial e interdisciplinar, afeito ao diálogo. A reflexão sobre o tema gerou um anteprojeto sobre o comportamento humano ante à transmissão de doenças de veiculação hídrica em municípios do Rio de Janeiro. Outro produto foi um texto em que são sugeridos caminhos para o diálogo
Facing diseases of social-environmental causes is frequently troubled by misunderstanding among communities, public power, and scientists, inspite of the availability of abundant and appropriate technical resources. This work is based on the experience of reflections about difficulties encountered by an interdisciplinary and multisectoral group and proposes dialogue as a method to prevent impasses between sectors, disciplines and subjects. The methodology consists of a process, still in progress, based on the consolidation of a multisectoral and interdisciplinary group accustomed to dialogue. Discussions of this theme generated the proposal of a project about human behavior regarding water-borne diseases in municipal districts of Rio de Janeiro. Another product was a text suggesting methods for achieving dialogue.
Subject(s)
Humans , Environment , Communicable Diseases, Emerging/epidemiology , Communicable Diseases, Emerging/prevention & control , Communicable Diseases/epidemiologyABSTRACT
Apresenta o caminho pelo qual temos evoluído em direçäo a uma soluçäo para os problemas de Guapimirim (Rio de Janeiro, Brasil) na área de saúde e ambiente. Objetiva atingir a partir de um estudo interdisciplinar e de uma parceria que vem assimilando, gradativamente, conceitos e práticas propostos por teóricos contemporâneos para lidar com complexidade, incerteza e avaliaçäo. Os conceitos de ciência "pós-normal", "comunidade ampliada de pares" e "transdisciplinaridade" servem de base para o entendimento do empreendimento científico em curso naquele município, e para a formulaçäo de uma proposta para sua continuidade.