ABSTRACT
PURPOSE: To evaluate the effects of aroeira (Schinus terebinthifolius) ointment on skin wound healing in rats. METHODS: Adult male rats (n=20) were divided into four groups of five animals each, as follows: G4, G7, G14 and G21, which corresponds to 4th, 7th, 14th and 21th days postoperatively. Each animal were made two incisions on the skin, including the subcutaneous tissue, in the right and left sides of thoracic region, separated by a distance of two inches. The right lesion was treated with base ointment (vaseline, lanolin); the left one was treated with base ointment containing 5% of aroeira oil. At the end of each experimental period the lesions were evaluated for the contraction degree. Then held the collection of fragments that were fixed in 10% formalin and processed for paraffin embedding. In the histological sections (5μm) was evaluated the morphology and quantified the collagen and blood vessels. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The contraction of the lesions was higher in wounds treated with aroeira oil than in controls at 7th and 14th days (p<0.01), whereas in the 21st day all lesions were already completely healed. The morphology showed granulation tissue more developed, with fibroblasts more bulky and collagen fibers more arranged in the experimental group at 4th, 7th and 14th days. The morphometry showed a significant increase in the quantification of collagen fibers in the experimental group at 7th and 14th days (p<0.05). CONCLUSION: The aroeira oil accelerates the healing process of wounds as a macroscopic, morphological and morphometrical analysis.
Subject(s)
Animals , Male , Rats , Anacardiaceae , Plant Extracts/therapeutic use , Plant Oils/therapeutic use , Subcutaneous Tissue/drug effects , Wound Healing/drug effects , Angiogenesis Inducing Agents/therapeutic use , Collagen/analysis , Immunohistochemistry , Phytotherapy , Postoperative Period , Subcutaneous Tissue/pathology , Time Factors , Treatment OutcomeABSTRACT
PURPOSE: To study the effects of the angico extract (Anadenanthera colubrina var. cebil) on the healing of rat skin. METHODS: Twenty adult rats were divided into four groups of five animals each, the G4, G7, G14 and G21, which corresponds to the respective postoperative days. Each group received two incisions on skin and subcutaneous tissue in the right and left antimere of the thoracic region, separated by a distance of 2 cm. The right lesion was treated daily with saline and the left with the angico alcoholic extract (5%). At the end of each experimental period, animals were euthanized and fragments of the wound area, together with the edges were removed, fixed in 10% formaldehyde solution and processed for paraffin embedding. In the histological sections with 5 µm of thickness, were carried out immunohistochemical methods for detection of blood vessels (VEGF) and stained with hematoxylin and eosin for morphological analysis. Statistical analysis was done by ANOVA and Tukey test (p<0.05). RESULTS: Morphological analysis showed larger fibroblasts and a higher concentration of collagen fibers in days 7 and 14 in wounds treated with the angico extract. Morphometric analysis demonstrated a significant increase in the number of blood vessels in both the seventh and 14th days (p<0.01) in wounds treated with the angico extract. CONCLUSION: The angico alcoholic extract (Anadenanthera colubrina var. cebil) induces the acceleration of wound healing in skin wounds of rats.
OBJETIVO: Avaliar os efeitos do extrato de angico (Anadenanthera colubrina var. cebil) na cicatrização em pele de ratos. MÉTODOS: Ratos machos adultos (n=20) foram distribuídos em quatro grupos de cinco animais cada, a saber: G4, G7, G14 e G21, o que corresponde a quatro, sete, 14 e 21 dias de pós-operatório. Cada grupo recebeu duas incisões na pele compreendendo o tecido subcutâneo, nos antímeros direito e esquerdo da região torácica, separadas por uma distância de dois cm. A lesão esquerda com extrato alcoólico de angico (5%), iniciando-se logo após a cirurgia por 21 dias consecutivos. Ao final de cada período (4, 7, 14 e 21 de pós-operatório) experimental foram coletados fragmentos da área da ferida, fixada em formol a 10% e processadas para inclusão em parafina. Nos cortes histológicos com 5 µm de espessura, foram realizados métodos imunoistoquímicos para detecção dos vasos sanguíneos (VEGF) e coloração pela hematoxilina para análise morfológica. Os dados obtidos foram submetidos à análise estatística ANOVA complementada pelo teste de Tukey-Kramer (p<0,05). RESULTADOS: A análise morfológica mostrou fibroblastos mais volumosos e alta concentração de fibras colágenas no 7º e 14º dias nas feridas tratadas com extrato de angico. A análise morfométrica demonstrou aumento significativo no número de vasos sanguíneos no sétimo e 14º dias (p<0,01) de pós-operatório em feridas tratadas com extrato de angico. CONCLUSÃO: O extrato hidroalcoólico a 5% da casca e entrecasca do angico (Anadenanthera colubrina var. cebil) acelera a neoangiogênese em feridas cutâneas de ratos.