The result of pharmacological studies on traditional medicine Chun-7 / Монголын эм зүй, эм судлал

Abstract@#CHUN-7 a Mongolian traditional recipe consisting of 7 medicinal plants is described in the scripture named “༆༆ །།གཡུ་ཐོག་པའི་མཛད་པའི་རིམས སྲུང་ཁྱུ་ལྔ་རྒྱམ་ཏོན་གསལ་བ །།”. The ingredients including Caowu (Radix <i>Aconiti kusnezoffii</i>, CW) and <i>Glehniae Radix</i> (GR) have the effects of suppressing pneumonia, viral pneumonia, relieving pain, stimulating the immune system and reducing fever. The present study was aimed to determine acute and chronic toxicity properties of traditional drug. @*Materials and methods@#CHUN 7 traditional drug were prepared in the traditional medicine sector of the Drug research Institute of Monos group. All WISTAR rats were kept in the same feed, under 12 hours lighting and 12 hours darkness housing. The study of acute and chronic toxicity of CHUN-7 traditional drug was studied by using IP and oral administration were performed on ten WISTAR rats and continued for a total of 28 days with 102.9 mg / kg dose (calculated from human dose: 16.7 mg/kg) in accordance with the WHO General Guidelines for the Evaluation and Study of Traditional Medicine.@*Results@#The result showed no structural changes in the internal organs according to tissue morphology when we administrated CHUN-7 traditional drug.@*Conclusion@#This study showed CHUN-7 traditional drug has no toxic effects to internal organs including liver, kidney, stomach, lungs and heart. Therefore, CHUN-7 traditional drug has potential to use orally without any toxicology.

The effect of “Cibo” ointment on mechanical wound in rats / Монголын эм зүй, эм судлал

Introduction@#In Mongolia, there is opportunity to replace importing products by using raw materials based on the animal and minerals. Those products can be used for medical purposes.</br> In traditional medicine, yellow marrow has been widely used to treat burns and mechanical wounds, as well as frozen and open wounds. The purpose of this study was to determine the pharmacological action of “CIBO” ointment. We created a model of mechanical wound to evaluate the healing of wounds and its index after applying the ointment contains the above mentioned raw materials. @*Material and methods@#Four different groups of “CIBO” ointment was prepared in the technology sector of the Drug Research Institute. Mebo wound ointment was used for comparison as control. The study was con- ducted in the Pharmacology Sector of the Drug Research Institute on white rats of the WISTAR breed under one feeding regime and one condition (12h / 12h). Mechanical wound injury and exposure to open wounds in experimental rats and its size were measured on the 3^rd, 7^th, 14^th, 21^st, and 28^th days of treatment. Wound healing, morphology, structure, size, and its index was quan- tified based on the defects. The ointment were applied once a day during the treatment period of 28 days to cover the wound.@*Result@#Mechanical wound showed that study treatment group healed 0-6.2% on the 3^rd day, 21.4-35.7% on the 7^th day, 50-60% on the 14^th day, and 33.3-50% on the 21^st day separately compared with the untreated group. On the 28^th day, all the groups were decreased by 66.6%. The wound index was between from 0.002 to 0.0005 in the all experimental groups. Among experimented groups, the group consists of yellow marrow and Chinese white cinder was showed better performance than other groups.@*Conclusion@#The study group 4 (Yellow marrow + Chinese white cinder) has been showed to have a pharmacological action of rapid healing and regeneration of burn wounds caused by experimental rats. It has also been shown to have pharmacological action to regenerate skin tissue and suppress caused by mechanicals. Inclusion of Chinese white cinder in “CIBO” ointment promotes regener ation and healing of “CIBO” ointment.

The effect of “Cibo” ointment on burn wound in rats / Монголын эм зүй, эм судлал

Abstract@#Burn injury causes considerable incidence of disability, increase of hospitalization and rehabilitation. It is a crucial factor of morbidity and mortality, throughout the world, especially in the developing countries. Burn wound healing is a complex process including inflammation, granulation, and regeneration of the skin tissue. There are many animal and mineral derived agents applied for burn wounds treatment. For example, minerals and animal yellow marrow are applied as standard topical therapy in healing of burn wounds in the history. Specially, Mongolian camel’s (<i>Camelus Bactrianus</i>) yellow marrow and cinders have been used the healing effect standard topical antimicrobial for burn wounds for several centuries. The present study aimed to determine the healing effect of “CIBO” ointment with animal and mineral raw materials on third -degree burn wound in rats.@*Introduction@#In our study, we selected an ointment containing a combination of yellow marrow and cinder. In ancient times, Mongolians used camel yellow marrow to relieve joint pain, spread people who had been bedridden for a long time due to the disease, and to prevent caries, and to use it on open wounds. In traditional medicine, cinder has been used as a mineral raw material and has been used to treat frostbite and burns.@*Material and methods@#In the Vivar Department of the Pharmacology Sector of the Drug research institute, 18 Wistar white rats weighing 182-220 g fed in one environment for (12 hours a day/12 hour a night) were randomly selected and divided into the following groups. These are: negative control, Group 1 “Yellow marrow + Uvs black cinder”, Group 2 “Yellow marrow + Uvs brown red cinder”, Group 3 “Yellow marrow + Chinese red cinder”, Group 4 “Yellow marrow + Chinese white cinder”, Standard “Mebo” wound ointment. The wound area was prepared by scraping the hair 5 cm from the back of the test animal’s skull. The prepared area was sterilized with 70% ethanol and 10% iodine solution, resulting in a third-degree burn wound of 2.4 cm2. Wound experiential’s ointments were applied once daily for a total of 28 days, and wound healing was evaluated at 0, 3, 7, 14, 21, and 28 days after pathogenesis, with 16.1-pixel accuracy. Photo confirmed using a SONY brand camera.@*Result@#“CIBO” ointment contains four deferent cinders which are from collected from deferent area and that have been shown to good affect burn wounds, and is more compatible with yellow marrow than Group 4 (Yellow marrow+Chinese white cinder).</br> Wound healing variables of control group compared with group 4 (Yellow marrow + Chinese white cinder) increased by 11.1% on the 3th day, 4% on the 7th day, 44.8% on the 14th day, and 54.5% on the 21st day. On the 28th day, healing and regeneration were accelerated by 50%. @*Conclusion@#The group 4 (Yellow marrow + Chinese white cinder) has been shown to have a pharmacological action of rapid healing and regeneration of burn wounds caused by experimental rats. It has also been shown to have pharmacological action to regenerate skin tissue and suppress inflammation caused by burns. Inclusion of Chinese white cinder in “CIBO” ointment promotes regeneration and healing of “CIBO” ointment.

Effect of “shilajit+golden rosa” or vitos preparation on immune response cells (CD4, CD8, IGG, CD64) by azathioprine induced immunosuppression in mice / Монголын эм зүй, эм судлал

Introduction@#During many decides, compounds derived from natural raw materials have demonstrated their effectiveness as therapeutic agents in different areas, such as metabolic disorder, immune system diseases and its regulations. Natural based products, like herbal medicines and minerals are implicated in the regulation of immune function. They control the immune system in a pleiotropic manner and participate in various processes of the adaptive/innate immunity. Therefore, natural raw material has great potential for targeted immune modulators, in the treatment of certain types of immunologic and inflammatory diseases, like rheumatoid arthritis, plaque psoriasis, ankylosing spondylitis and immune deficiency. The purpose of this survey was to study influence of “Shilajit +Golden Rosa” combined shot preparation named by Vitos on immune system in the experimental and preclinical circumstances.@*Goal@#The purpose of this survey was to study influence of “Shilajit +Golden Rosa” Vitos shot preparation on immune system in the experimental and preclinical circumstances.@*Material and Methods@#The immune deficiency was to created by Azathioprine through 5 days in the Balb/c mice after that control group, preparation of “Shilajit +Golden Rosa” Vitos shot were administrated appropriate doses by oral during 10 days. Then we collected blood and quantified amount of CD4+, CD8+, IgG and CD64 (Mouse Elisa Kit Assay: Catalog.No:WAM-568, Elisa Reader, 450 <b>нм</b>, Melsin Medical Co.LTD, www. melsin.com) on the 5^th, 10^th days.@*Results@#All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). Oneway ANOVA was used to assess statistical significance between “Shilajit +Golden Rosa” Vitos shot group and days of observation. Mean values of CD4+, CD8+, CD4/CD8 ratio, IgG, CD64 levels determined in the control and sample group. CD4+, CD8+, CD4/CD8 ratio, IgG and CD64 levels were significantly increased in the “Shilajit +Golden Rosa” Vitos shot group compared with control group by 20.8-67.8 per cent (p<0.05, p<0.01).@*Conclusion@#It’s concluded that, “Shilajit +Golden Rosa” Vitos shot preparation shows immune-stimulator activity not only in the level of cellular (T cells:CD4+, CD8+) but also humoral immunity (B cells: IgG, CD64) in the previously using Azathioprine (75mg/kg) to provoke pathological model of immunosuppression

The results of Hippophae Rhamnoides L fruit processing technology / Монголын эм зүй, эм судлал

Introduction@#Sea buckthorn (<i>Hippophae rhamnoides</i> L.) is a hardy deciduous shrub of family <i>Elaeagnaceae.</i> In traditional medicine, “Sea buckthorn-5” powder medicine and sea buckthorn extract for the treatment of lung diseases,“Sea buckthorn-11” and “Sea buckthorn-17”prsecriptions are used to treat gynecological diseases. Sea buckthorn fruit takes a lot of time to dry and prepare in the traditional way, and a small amount of raw material is obtained. Therefore, there is an urgent need to improve and standardize technology.@*Material and method@#The study used “Sea buckthorn fruit” raw material harvested in September 2020 from the Botanical garden of medicinal plants of the Drug research institute and sea buckthorn dry extract purchased from China.Four types of samples were used as Dry fruit of sea buck- thorn (Sample 1), Sea buckthorn seeds (Sample 2), Natural dried sea buckthorn fruit (Sample 3), and sea buckthorn dry extract purchased from China (Sample 4).In each of these four samples, the total carotenoid was measured at 450 nm, the flavonoid at 500 nm, and the phenolic compound at 750 nm using a spectrophotometer.@*Result@#The results show that Sample 1 contains the highest amount of carotenoids 56.29 ± 0.05%, Sample 2 contains the highest amount of flavonoids 32.19 ± 0.05%, and total phenolic compounds 41.67 ± 0.02%.@*Conclusion@#Dry fruit of sea buckthorn (Sample 1) has the highest content of carotenoids, sea buckthorn seeds (Sample 2)have the highest total flavonoids and total phenolic compounds, which is approximately to the content of natural sea buckthorn fruit.

Determination of inuline contents in heat and cold processed Helianthus Tuberosus L / Монголын эм зүй, эм судлал

Introduction@#<i>Helianthus tuberosus</i> L. is main source of inuline in pharmaceutical and food industry. Pharmacological studies of the plant have showed some important therapeutic properties such as anti-diabetic, anti-osteoporosis, anti-cancer and strengthening cardiovascular system, immune system.@*Material and method@#<i>Helianthus tuberosus</i> L., which has been cultivated in Botanical Garden of Medicinal Plants of Drug Research Institute, Monos Group, was used as a research raw material. The aerial and below ground parts of the plants were harvested in September 2020. The content of inulin, the main active ingredient in the plant, was determined by spectrophotometry. Raw materials of the plant were processed in several ways and prepared for further use as a pharmaceutical raw material.@*Result@#Inulin content in Sample 1 was 34.5 ± 0.76%, Sample 2 was 70.31 ± 1.25%, Sample 3 was 78.43 ± 0.44% and Sample 4 was 75.36 ± 1.42%. The inulin content and yield were the highest in samples prepared by heat and cold cutting methods. @*Conclusion@#According to the results of appearance, yield, inulin content and moisture contents during the different processings of plant materials, it can be considered that the most suitable method for preparing <i>Helianthus tuberosus</i> L. as a pharmaceutical raw material is the cutting followed by freeze-drying.

To evalate the effect of “Vitos” shilajit shot preparation on rat femur fracture using X-ray / Монголын эм зүй, эм судлал

Introduction@#Shilajit and Rhodiola Rosa L are widely used in Mongolian Traditional medicine for the management of diseases and for fracture healing. The aim of this study was to evaluate the pharmacology effects of the “Vitos” Shilajit Shot preparation on fracture healing and callus stages in rats by X-ray.@*Material and methods@#We used non-liner Wistar rats for <i>in vivo</i> experiments, there are sixteen rats were randomly grouped as a positive control, negative control, “Vitos” Shilajit shot experimental and standard groups. The positive group was as healthy animals and other groups were created femoral fracture by Bonnaren’s device. Then negative control group was oral administered distilled water, whereas 4.1ml/kg of “Vitos’ Shilajit shot administrated via oral gavage to experimental group through 56 days. X-rays were performed to assess fracture healing effects within 14, 28, 42, 56 days and callus stages.@*Results@#Significantly higher callus volume and callus staging were observed in the “Vitos” Shilajit shot group compared with the negative control and standard groups. Also “Vitos” Shilajit shot group was becoming as bridging between both end of fractures and get hard callus formulation ready observation of X-Ray radiograph on 4 weeks post fracture. The fracture healing process was slightly reached to callus remodulation such as final stage of bone formulation on 56^th day.@*Conclusion@#The results of this study reveal that, “Vitos’ shot preparation, which contains an extract of <i>Rhodiola Rosa L</i> and thick extract of Shijilat has a treatment effect and enhancing and supporting callus of bone fracture healing.

Method validation of total iridoids in “Darmon” tablets by spectrophotometric method / Монголын эм зүй, эм судлал

Introduction@#A joint research team of the Drug Research Institute аndMonos pharm Co.ltd is conducting an experiment to produce of “Darmon” tablets.Idridoids are one of the predominant biological active compound in “Darmon” tablets and will be an important indicator of the quality of the drug.@*Objectives@#This is the first report on the determination of iridoids by spectrophotometric method in “Darmon” tablets.@*Methods@#The amount of total iridoids of “Darmon” tablets was confirmed by spectrophotometry and the absorbance was measured at 238 nm. Geniposide (98%, Xilong Scientific Co., Ltd) was used as the standard substance.@*Results@#The developed spectrophotometric method showed good linearity (R²=0.9989), high precision (RSD<2%) and a good recovery (96.01-104.48%). All the validation parameters of the spectrophotometric method were found to be within the permissible limits according to the ICH guidelines. @*Conclusions@#The method was robust, accurate and reliable for the quality control of “Darmon” tablets.

Method validation of hydroxysafflor yellow A in “Carthamus tinctorius” injection by High-performance liquid chromatography / Монголын Анагаах Ухаан

Introduction@#Carthamus tinctorius L. widely accepted as Safflower or false saffron, belongs to the Compositae or Asteraceae family. Hydroxysafflor yellow A is the main active chemical compound present in florets of Carthamus tinctorius L. A joint research team of the “Tsombo Pharm” Co., LTD and the Drug research Institute is conducting an experiment to produce a solution of “Carthamus tinctorius” injection prepared by Carthamus tinctorius L. @*Goal @#The aim of this study was to develop the validation method of hydroxysafflor yellow A in “Carthamus tinctorius” injection.@*Material and Methods @#As a test sample “Carthamus tinctorius” injection was produced by “Tsombo pharma” Co., LTD. The standard Hydroxysafflor yellow A was supplied from Sigma-Aldrich Co., Ltd. The reagent were high-performance liquid chromatography grade acetonitrile, phosphoric acid, methanol and purified water. </br> Shimadzu HPLC (CMB-20 A, UV detector Shimadzu SPD-20A was used as the analytical instrument and the analysis conditions were as follows Table 1. @*Results@#A Shimpack С18 column was used with methanol:acetonitrile:0.7% phosphoric acid as the mobile phase under the condition of gradient elution. The hydroxysafflor yellow A were analyzed by using a timed wavelength measure according to their maximum absorption wavelength. Accuracy and precision were assessed by analyzing five sets of samples, independently prepared at low (50%) middle (100%) and high (150%) concentrations. The intraday and interday precisions of the investigated compound were less than 1.59 % and the average recoveries ranged from 81.9% to 101.5%. </br> There were good linear correlations between the concentrations of the hydroxysafflor yellow A and its chromatographic peak areas (R2 = 0.998), the proposed method was successfully applied to determine the hydroxysafflor yellow A in “Carthamus tinctorius” injection. @*Conclusions@#The results indicated that the proposed method is simple, stable, and accurate and could be readily utilized as a quality control method for manufacturing process of “Carthamus tinctorius” injection.

Technological study to convert traditional powder medicine into modern dosage formulation / Монголын Анагаах Ухаан

Introduction@#The Mongolian people have been using traditional medicine for hundreds of years. However, there is a need to produce traditional medicinal dosage forms such as capsules, tablets, decoctions easier to drink, store and transport, and to standardize not only finished product, but the raw materials used for traditional medicines in line with the current drug production, drug quality and safety requirements. Therefore, in this study, we aim to standardize five ingredients of a traditional powder medicine, which have been widely used for colds and flu in Mongolian, Chinese and Tibetan traditional medicine practice, and to convert the powder drug into tablet form using qualified raw materials.@*Materials and Methods@#The study to convert multi-ingredient traditional powder into tablet was carried out at the Experimental production pharmaceutical technology unit and Pharmaceutical chemistry Laboratory of the Drug research institute, Monos Group, and the Quality control laboratory of medicine of Monos Pharm LLC. @*Result@#All raw materials were standardized and wet granulation method was used to prepare granules for the tableting with qualified raw materials. 4 different models of the tablet form was prepared and investigated. It was determined that model X-2 fully meets the general requirements for the tablet form.

Method validation of pyridoxine hydrochloride in neurorubin by high performance liquid chromatography / Монголын эм зүй, эм судлал

Introduction@#Pyridoxine hydrochloride is least expensive supplement named as a vitamin 6¹. Pyridoxal kinase is the enzyme that produces pyridoxal phosphate which known as pyridoxine hydrochloride that occurs in the human body. Diabetes, age and neurodegenerative diseases complications can be reduced by pyridoxine hydrochloride^{2 3}. Quantification of pyridoxine hydrochloride in neurorubin as an injection form was developed by high performance liquid chromatography (HPLC) method. Further, the proposed method was validated for linearity, precision (system precision, method precision, intermediate or inter-day precision), and accuracy, stability in analytical solution, system suitability and roughness. The developed method exhibited the best results in terms of the aforesaid validation parameters. The method was found to be selective, simple, economical, accurate, reproducible, rapid and reliable for routine estimation purpose of pyridoxine hydrochloride in injection.@*Goal@#The aim of this study was to develop the validation method of pyridoxine hydrochloride in injection. @*Material and Methods@#</br>I) Test Article. As a test article neurorubin injection was produced by Tsombo Farm LLC. The standard pyridoxine hydrochloride was supplied from Sigma Aldrich Co. </br>II) Reagents and Equipment. The reagents were HPLC grade acetonitrile methanol, and purified water. Balance, and micropipette used as equipment. Shimadzu HPLC (LC20AD) was used as the analytical instrument and the analysis conditions were as follows (Table 1). @*Results@#The calibration curves for pyridoxine hydrochloride were made by plotting the peak area versus the concentration for each analyte using regression analysis. Each calibration curve was obtained using six levels of concentrations in the range 12.5-100 pg/mL. The linear correlation coefficient (R²) for all calibration curves was higher than 0.995 for all analytes. The LOD and LOQ for pyridoxine hydrochloride were in 15.29 pg/mL and 46.33 pg/mL, respectively.</br> Accuracy and precision were assessed by analyzing five sets of samples, independently prepared at low, middle and high concentrations. The RSD values of both repeatability and intermediate precision were below 1.669 % and 1.678 % the accuracy remaining between 95.25 to 102.775 %. The resulting accuracy data were satisfactory for the quantitative analysis of pyridoxine hydrochloride in neurorubin injection.</br> The results of summarized in table 2, 3, 4. This article presents a simple, accurate, reproducible, and thoroughly validated HPLC-based method for qualitative and quantitative analysis of pyridoxine hydrochloride, as part of the quality assessment of products containing in injection.

The role of CD28, CD40, CD45 RO and immunoglobulin G(FcyRI, FcyRII) in expression of T and B cells in mice / Монголын эм зүй, эм судлал

Purpose@#In generally, T cell activation evaluates by CD4 +, CD8 +, B cell immunoglobulin's (IgG, IgM, IgA) in Azathioprine caused secondary immunodeficiency in serum. May it does not make clearly interpret for regulation of cell-mediated and humoral immune response using interaction T and B cells. In this review, we discuss the multifaceted roles of cytokines as enhancers and expression of B and T cell. @*Cell-mediated Immunity and Humoral Immunity@#Based on the type of immunodeficiency, the types of defects can vary. While some bacterial infections may be a key feature of B cell and T cell defects are feature of combined T and B cell immunodeficiency. Also other defects can be cause of phagocytes cell and complement deficiency. The present study provides evidence special cytokines which are involved to expression of T and B cells that CD40, IgG, IgM, FcyRII (CD32) need to be define with B cell deficiency, CD40, CD45RO define combined T and B cell deficiency and FcyRI (CD64), CD45RO define for phagocytes cell deficiency and CD4+, FcyRI (CD32), FcyRI (CD64) define for complement deficiency. In this review, we have done pharmacological study of immunosuppressant injection which is with Mongolian <i>Astragalus Mongolicus</i> Bge and determined CD28, CD40, CD64, and CD45 cytokines in the blood serum of mice. It will be clarifying the immune system interaction between cell-mediated immunity and humoral immune for T cells and B cell interactions.

Result of nephroprotective effect of aqueous extracts of Iris Tenuifolia Pall and Iris Lactea Pall / Монголын эм зүй, эм судлал

Background@#<i>Iris Tenuifolia</i> and <i>Iris Lactea</i> known for its various medicinal properties are also a natural antichloristic and a kidney protective as agent.@*Goal@#To evaluate the nephrite activity of aqueous extract of <i>Iris Tenuifolia</i> and <i>Iris Lactea</i> in a rodent model of kanamycin induced nephrotoxicity. @*Materials and Methods@#In the experimental design, thirty-six Wistar rats were randomly isolated into four groups of one control and three experimental. Nephrotoxicity in rats induced by intramuscular injection of Kanamycin {250 mg/kg) daily for 5 days⁵. The doses of 25 mg/kg, 50 mg/kg, 75 mg/kg, 100 mg/kg of aqueous extract of <i>Iris Lactea</i> and dose of 25 mg/kg <i>Iris Tenuifolia Pall</i> were administrated by oral gavages for 14 consecutive days in rats. At 14 days for the rest of them, serum samples were collected for renal function biochemical tests (Creatinine, Creatinine Clearance, Urea UV and GFR-Glomerulus Filtration Rate). @*Results@#All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armork. NY). One-way ANOVA was used to assess statistical significance between experimental groups and control group. Mean values of creatinine, creatinine clearance, urea UV and GFR levels determined in the control and experimental groups. Kanamycin treatment caused nephrotoxicity as evidenced by marked elevation in serum creatinine, creatinine clearance, GFR and urea UV, <i>Iris Tenuifolia</i> 25 mg/kg blood serum creatinine (62.49±1.24 (38%), 56.38±1.41 (4.5% μmol/L), serum creatinine clearance (4.79±0.16 (45%), (5.80±0.36 (6%) ml/minute), serum GFR (191.6±6.58 (45%). (232±14.65 (5.9%) ml/minute), serum urea UV (8.64±0.63 (9.6%), (8.40±0.07 (20.23%), Iris Lactea 75 mg/kg blood serum creatinine 68.92±4.08(31%), 58.87±1.95 (0.4% μmol/L), serum creatinine clearance (5.27±0.67(60%), (5.67±0.28(3.6%) ml/minute), serum GFR (210.9±26.78 (60%), (226.8±11.28 (3.5%) ml/minute), serum urea UV (7.73±0.58 (19.14%), (7.48±0.35 (28.96%) respectively when compared to the control treated groups. Oral administration of <i>Iris Lactea</i> 75 mg/kg extract decreased the rise in these parameters in a dose dependent manner. @*Conclusion@#Our studies suggest that aqueous extract of <i>Iris Lactea</i> 75 mg/kg and <i>Iris tenuifolia</i> 25 mg/kg results are shown good effect for anti-inflammatory of renal.

Method Validation of Dihydromyricetin in Anti-hangover preparation by High Performance Liquid Chromatography / Монголын Анагаах Ухаан

Background@#The high performance liquid chromatography (HPLC) method was developed for selective determination of dihydromyricetin in capsule formulation dietary supplement containing other components. Further, the proposed method was validated for linearity, precision (system precision, method precision, intermediate or inter-day precision), and accuracy, stability in analytical solution, system suitability and ruggedness. The developed method exhibited the best results in terms of the aforesaid validation parameters. The other components and additives did not interfere in their determinations. The method was found to be selective, simple, economical, accurate, reproducible, rapid and reliable for routine estimation purpose of dihydromyricetin in dietary supplement capsule.@*Goal @#The goal of this study was to develop the validation method of dihydromyricetin in the dietary supplement.@*Material and Methods @#The hangover preparation was produced by Technological section of Drug Research Institute. The standard dihydromyricetin was supplied from Sigma Aldrich Co. We used solvents for HPLC grade (methanol, acetonitrile). Chromatographic conditions: A gradient HPLC (Shimadzu LC20AD) with serial dual plunger pump; analytical column: Supelco inertsil С18 250 × 4.6 mm, particle size 5 μm; flow rate: 1 ml/min; column temperature: 350C, detection: UV 365 nm. Chromatographic procedure: 20 μl of the mixed standard preparation and assay (sample) preparation were separately injected into the chromatography, the chromatograms were recorded, and the responses for the major peaks were measured. The run time was approximately 10 minutes.@*Results @#The calibration curves for dihydromyricetin were made by plotting the peak area versus the concentration for each analyte using regression analysis. Each calibration curve was obtained using six levels of concentrations in the range 28-224 µg/mL. The linear correlation coefficient (r2 ) for all calibration curves was higher than 0.999 for all analytes. The LOD and LOQ for dihydromyricetin were in 11.29 µg/mL and 34.21 µg/mL, respectively. Accuracy and precision were assessed by analyzing five sets of samples, independently prepared at low, middle and high concentrations. The RSD values of both repeatability and intermediate precision were below 0.261% and 0.262%. The accuracy remaining between 101.65 to 104.7%. The resulting accuracy data were satisfactory for the quantitative analysis of dihydromyricetin in anti-hangover preparation. The results of summarized in Table 1, 2, 3. This article presents a simple, accurate, reproducible, and thoroughly validated HPLC-based method for qualitative and quantitative analysis of dihydromyricetin, as part of the quality assessment of products containing anti-hangover preparation.

Effect of Immunos on immune response cells by azathioprine Induced immunosuppression in mice / Монголын Анагаах Ухаан

Background@#Herbal medicines continue to be widely used as natural promoters of good health, as immune-modulators in recent years. This situation is directly related to the rapid growth of natural based products, the decrease of chemical synthesized products and as well as the increase of natural substance consumption. @*Objective@#The purpose of this survey was to study influence of Immunos herbal medicines on immune system in the experimental and preclinical circumstances.@*Materials and Methods@#The immune deficiency was to created by Azathioprine through 5 days in the white mice after that control group, preparation of Immunal, Salimon and Immunos 1, 2 were administrated appropriate doses by oral during 10 days. Then we collected blood and quantified number of white blood cells (K/µL), quantity of splenocyte (×106 cell/ml), amount of CD4+, CD8+ and IgM, IgA, Ig G (mg/ml) (Elisa Kit Assay: Catalog. No: WAM-568 (Elisa Reader, 450 nm)-WKEA MED SUPPLIES CORP) on the 5th, 10th days.@*Results@#All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). One-way ANOVA was used to assess statistical significance between Immunos groups and days of observation. Mean values of white blood cells in blood, quantity of splenocyte, CD4+, CD8+ and IgM, IgG levels determined in the control and experimental groups. White blood cells level were significantly increased in the Immunos group compared with the control group by 55.6 percent (11.5±0.9 K/µL vs 5.1±0.51 K/µL, p<0.001) and number of splenocyte increased Immunos group compared with the control group by 60.6 % (352.2±23.5 ×106 cell/ml vs 138.6±23.5 ×106 cell/ml, p<0.01). Therefore, CD4+, CD8+ and IgM, IgG levels were significantly increased in the Immunos group compared with the control group by 0.71 to 8.8% (IgG: 11.47±0.42 vs 10.45±0.43 μg/ml, IgM: 11.33±0.81 vs 10.48±0.31 μg/ml, CD4+: 10.44±0635 vs 10.04±0.372 U/ml, CD8+: 9.75±1.02 vs 9.68±0.45 U/ml p<0.02).@*Conclusion@#It’s concluded that, Immunos preparation shows immune-stimulator effect in cellular immunity and humoral immunity in the case of immunosuppressant by Azathioprine.

A dynamic of some biological active compounds in the Seabuckthorn leaf / Монголын Анагаах Ухаан

Background@#Selected three sorts of Seabuckthorn have been being planted since 2000 in the Botanical garden of Medicinal plants of “Monos” group, Mongolia. The chemical compositions of leaves were investigated from May 2017 to October 2017. In study, we had sampled at several month and determined content of tannin, total flavonoid, total organic acid and polysaccharide and antioxidant activity during growing seasons.@*Goal@#The purpose of this study is to determine how does change biological active compounds in the Sea-buckthorn leaves that depending on growing season and to describe which harvesting period is efficient to use. Seabuckthorn leaf samples were prepared from such as: Chuiskaya, Oranjivaya and Obilniya on their chemical composition depend from harvesting season.@*Materials and Methods@#The chemical contents were analyzed with the colorimetric assay, volume metric analysis method and spectrophotometric method. @*Result@#For sorts we studied, content of tannin was 17.66% in Orangevaya sort, total content of flavonoid was 0.39% in Chuskaya sort, total antioxidant activity was IC50- 9.38 mg/ml in Obilnaya sort, total acid content was 2.4% in Orangevaya sort, and content of Polysaccharide was 10.24% in Obilnaya sort.@*Conclusion@#The results from this study, there was shown differences in bioactive substance of each sorts, overall content of tannin July to August, total flavonoid May to July, and antioxidant activity in June, were high.

A dynamic of some biological active compounds in the Iridaceae species / Монголын Анагаах Ухаан

Background @#We have done dynamic study of tannin and total flavonoid contents in samples of Iridaceae such as Iris bungei maxim, Iris tenuifolia Pall, Iris lactea Pall and Iris dichotoma from 2017 April to 2017 Oct. Those 4 samples were prepared from Khuld sum of Dundgobi, Tsogttsetsii sum of Umnugobi, Altanbulag soum of Tuv province and the Botanical Garden 2 of Medicinal plants of Drug Research Institute. @*Goal@#The purpose of this study is to determine how does change biological active compounds in Iridaceae species that depending on growing season and to describe which harvesting period is efficient to use. @*Materials and Methods@#The chemical contents were analyzed with the colorimetric assay, volume metric analysis method and spectrophotometric method.@*Result@#In result of study, there was shown differences in bioactive substance of each Iridaceae, overall content of polyphenolt compound and total flavonoid highest from April to June. But, the bioactive substance in the Iris dichotoma was the highest in August and constantly increased in May to August then constantly decreased in September and October. Comparisons between Iridaceae; the highest concentration of bioactive substance was Iris tenuifolia Pall and the lowest concentration bioactive substance was Iris lactea Pall.

Results of determination Ginsenoside of Panax ginseng cultivated in Mongolia / Монголын Анагаах Ухаан

BackgroundPanax ginseng is one of the most important medicinal plants in Asia. Triterpene saponins(ginsenosides) are the main bioactive compounds in P.ginseng. The present study investigated thegrowth characteristics of ginsenoside content in the leaves and roots of Panax ginseng at differentgrowth stages (from 1 and 4 years). Analysis was P.ginseng leaves and roots indicated the presenceof two ginsenosides (Rg1 and Rb1) content of Rg1 was higher than Rb1.Aim: The main aim of this study was determine by high performance liquid chromatography (HPLC)ginsenoside Rg1 an Rb1 of P.ginseng grown in Mongolia.Materials and MethodsLeaves and roots of different ages of P.ginseng were collected at October of 2015 in field of Umnugoviprovince, Mongolia. Collected samples were dried and powdered. Samples were extracted with70% EtOH, water saturated butanol and methanol. The extract was filtrated through filter paper(whatman No.42) and evaporated vacuum rotor. The evaporated extract was resuspended with theMethanol. It stored in room temperature and resuspended mobile phase use for HPLC analysis.The two ginsenosides were analyzed using HPLC system of a model (DGU-20A3r Shimadzu) andcolumn was Octadecylsilane 5μm I-150mm, D-4.6mm, detector was UV 204 nm. The sample wasinjected (20μl) and applied gradient elution was as follows British pharmacopeia.ResultsGinsenosides levels were much higher in the 4 ages roots compared with the 1 ages roots.Ginsenoside amounts in P.ginseng organs changed depending on the specific time during thevegetation season the samples were taken. This study found that the highest content of thesemetabolites-2.082% (butanol extract) occurred in the roots. Our study was independently of thevegetation season. These were Rb1 and Rg1, with values Rg1 was 0.7-2.082% and Rb1 was 0.002-0.03%.ConclusionGinsenosides are generally distributed throughout all the parts of the ginseng plant. We found thatthe highest Ginsenoside Rg1 content accumulated during the first year of growth then decreaseduntil four year.

Pharmacological studies of anti-hangover preparation / Монголын Анагаах Ухаан

IntroductionA hangover is the experience of various unpleasant physiological and psychological effects followingconsumption of alcohol beverages, which can last for more than 24 hours. Common symptoms ofhangover are headache, gastrointestinal complaints, sweating, hyper-excitability, dry mouth, anorexia,diarrhea, dizziness, fatigue and vertigo. Alcohol or ethanol gets metabolized to an intermediate product,acetaldehyde, by the enzyme alcohol dehydrogenase (ADH), and then acetaldehyde is converted toacetate by a second enzyme aldehyde dehydrogenase (ALDH). Acetaldehyde causes toxic effects,such as high pulse, rate, sweating and vomiting. In most people, ALDH metabolizes acetaldehydequickly and effi ciently, so that this intermediate metabolite does not accumulate in high concentrations.Many treatments are described to prevent hangover, shorten its duration, and reduce the severity of itssymptoms, including innumerable folk remedies and recommendations.GoalThis study was conducted to investigate whether anti-hangover preparation has a protective effectagainst acute alcohol induced hangover in Wistar rats.Materials and MethodsMale and female Wistar line rats, weighing 180-210g were used for hangover model or ethanolmetabolism experiment. Rats were administered orally ethanol as 38% aqueous solution with feedingneedle, 1 ml/200g body weight. The anti-hangover preparation was administered 1 hour before ethanolconsumption. Blood was collected from the tail vein for the measurement of serum acetate andacetaldehyde at just before and 8, 16, 24 hour after ethanol administration.Statistical analysis: All value expressed as mean S.E obtained from n number of experiments.ResultFrom this study results summarize that the anti-hangover preparations decreased blood serum acetateand acetaldehyde levels as compared to control. Anti-hangover preparations enhanced acetaldehydeand acetate metabolism.Conclusion: These fi ndings indicate that anti-hangover preparations may exert benefi cial role inthe treatment of alcohol hangover without any toxicity. Therefore, the content of acetaldehyde wasdecreasing and increasing through repeating 8 hours within 24 hours.

"Some Results of the Marketing Research for “Enap” Medicine that’s / Монголын эм зүй, эм судлал

Background: Today over 1 million people have hypertension in the world. 1.5 billion people have symptoms of the diseases living difficulty. The hyperpiesis is 64% brain hemorrhage causes, and 49% of heart attack diseases. Blood pressure or hyperpiesis is the leading cause of adult diseases in Mongolia. With hypertension diagnoses, 19811 people hospitalized in patient treatment and 46022 people have received outpatient care through Mongolia. The most present of Mongolians regularly use medicines for reducing arterial pressure. In this research, we chosen the Enap medicine that is used common; 5 mg, 10 mg and 20 mg Enap are used at imported and domestic industry in the pharmaceutical market, Mongolia. Aim: To research some indicators of Enap pharmaceutical market which is used common in the treatment by Mongolians. Method: Based on the statistics from Mongolian Customs, when we research about the Enap market, we used the drug economic analysis methods including comparative research methods and content analysis research methods, respectively. Results: Our result shows that the 66.7% of all Enap in Mongolian pharmaceutical market is imported, and other 33.3% medicine is produced in domestic manufacturers, Mongolia. In Mongolia, 8 companies imports the Enap medicines from the biggest farms like KRKA, Denk Pharma, Berlin Chemie and Akrihin-akri. In the comparison research for Enap sales, there are 8 importer companies, the Х1 company sells the most, and it’s 22.4 times much than the lowest sales. In the research for Enap /10mg/average prices, the imported products cost 324 MNT but the domestic products cost 1525 MNT.