1.
Rev. argent. microbiol
;
33(4): 203-8, 2001 Oct-Dec.
Article
in Spanish
|
LILACS-Express
| LILACS, BINACIS
| ID: biblio-1171693
ABSTRACT
Two monoclonal antibodies (MnAb) directed against the lipopolysaccharide (LPS) and major outer membrane protein (MOMP) of Chlamydia trachomatis were produced for use in indirect immunofluorescence (IFI). The specificity of the antibodies was determined by Dot-blot, Immunoblotting (IB) and IFI onto culture cells infected with C. trachomatis and IFI onto commercial swabs (MRL). The MnAb 2D3 and 3C2 detected LPS and MOMP of C. trachomatis, respectively, by different methods. Neither MnAb showed cross-reactions when other gram-negative bacteria were used as antigens.