ABSTRACT
Alternol and Alteronol are two small chemical compounds purified from a mutant fungal.The in vitro and in vivo experimental studies have confirmed their potent anti-cancer effect.In vitro studies show that they can inhibit cell division and proliferation of a variety of tumors including prostate cancer,osteosarcoma and melanoma.They exert their anti-cancer effects by attenuating cell cycle protein expression and causing cell cycle arrest,suppressing tumor cell invasion and metastasis,reducing new blood vessel formation,decreasing the expression of anti-apoptotic proteins Bcl-2 in malignant cells,and stimulating oxygen free radical accumulation.However,there is no significant cytotoxic effect on benign cells.Animal experiments show that these two compounds significantly inhibit xenograft growth derived from a variety of human malignant tumors,while there is no obvious side effect on normal tissue or organs.In conclusion,these two compounds are worthy to be developed as novel anti-cancer drugs.
ABSTRACT
Objective To identify the effective results of ultrasound in degradation of polymeric nanoparticles released DNA .Polymeric nanoparticles was made by dehydration of polyacetylglutamicacid (PLGA, polylactic-co-glycolic acid)solution. Method Green Fluorescent Protein (GFP) was enclosed by PLGA. Different kinds of ultrasound mode and different duct cycle and power ones were used to radiate PLGA solution for 90 s, 9 min, 20 min separately after the solution prepared for 2 hrs,then putted the solution on centrifugal machine at 13000 r/m. Using Choloroform to get rid of fat-soluble impurity,then applied nanodrop to survey the releasing rate of DNA. Finally the effect of cell expression were observed by fluorescent microscope. Results The amount of DNA released from PLGA in groups which were exposed to ultrasound were significantly different from the groups which were not exposed to ultrosound. The releasing amount of former groups had upper limitation. The releasing rate was increased with the increment of the irradiation time,frequency of ultrasound;The effect of the DNA releasing and PLGA degradation by continuous-wave irradiation was stronger than pulsed-wave ultrasound. Conclusion Ultrasound can promote the degradation of PLGA, and do help in DNA releasing and expression in vitro.