ABSTRACT
Se evaluó la calidad bacteriológica del agua de pozo y del agua de lavado en una muestra aleatoria de 50 tambos distribuidos en la cuenca lechera de Villa María (Córdoba), Argentina. La visita a los tambos se realizó en 2007. Un 46 % y un 24 % de los tambos presentaron recuentos de aerobios mesófilos superiores a 500 UFC/ml en el agua de lavado y en el agua de pozo, respectivamente. En un 20 % de los establecimientos se aisló Escherichia coli de ambas fuentes de agua. Pseudomonas aeruginosa registró una alta frecuencia de aislamiento en el agua de pozo (36 %) y en la de lavado (42 %). Un 80 % y un 88 % de los establecimientos contaban con agua de pozo y de lavado no aptas, respectivamente. Los niveles de mesófilos aerobios y de coliformes totales presentes en el agua de pozo mostraron una concordancia moderada con los detectados en el agua destinada al lavado. En virtud de estos resultados, puede afirmarse que un elevado porcentaje de los tambos ubicados en la cuenca lechera de Villa María emplean agua de calidad bacteriológica deficiente, no apta para el ordeño ni el lavado de las instalaciones.
Bacteriological contamination of well water and wash water in a random sample obtained from 50 farms from Villa María (Córdoba) dairy area, Argentina, was evaluated during a visit in 2007. Forty six percent and 24 % of farms showed an aerobic mesophilic bacteria count higher than 500 CFU/ml in wash water and well water, respectively. Escherichia coli was isolated in 20 % of samples from both sources. Pseudomonas aeruginosa showed high frequency of isolation in well water (36 %) and wash water (42 %). Eighty and eighty-eight percent of dairy farms have contaminated well water and wash water, respectively. The findings show moderate concordance between contamination of well water and wash water for mesophilic aerobics and total coliforms. The results reveal that a high percentage of dairy farms in the basin under study have poor bacteriological water quality, not suitable for milking and washing facilities.
Subject(s)
Animals , Cattle , Dairying , Water Microbiology , Water Pollution , Water Supply/standards , Argentina , Bacteria, Aerobic/isolation & purification , Enterobacteriaceae/isolation & purification , Escherichia coli/isolation & purification , Pseudomonas aeruginosa/isolation & purification , Sampling Studies , Waste Disposal, FluidABSTRACT
The aim of this study was to assess by rep-PCR the genetic relationshipof 52 S. aureus strains isolated from mammary infections collected infour herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At afirst level of similarity (50%), it could be defined 5 clusters namely Ito V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution indairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections.
O objetivo do presente estudo foi determinar a relação genética entrerep-PCR de 52 linhagens Staphylococcus aureus isoladas de infecçõesmamárias em quatro fazendas leiteiras da região leiteira central daArgentina. Os resultados foram comparados com a atividade in vitrodos antimicrobianos freqüentemente utilizados no tratamento da mastite bovina. Foram observados 12 diferentes perfis deantimicrobianos. Do total de linhagens, 45% foram suscetíveis atodos os antibióticos ensaiados. A caracterizacão por rep-PCR podediferenciar com sucesso as linhagens de S. aureus de origem bovina.Num primeiro nível de similaridade (50%) foram definidos cincogrupos denominados de I a V. A maioria das estirpes (75%)agruparam-se no grupo I. Os resultados sugerem que os genotiposforam similares. Os genotipos não foram asociados com os perfis deantimicrobianos na maioria dos isolados. O presente estudo descreveos genotipos responsáveis pelos casos de mastites na região central daArgentina. O melhor conhecimento da distribução das linhagensinfectantes em fazendas leiteras poderia auxiliar na formulacão deestratégias para o controle de infecção. Além disso a suscetibilidadeaos antimicrobianos de linhagens de S. aureus deve ser usada paramontear a selecão de drogas efetivas para a terapia intramamária.