ABSTRACT
Background: Lactate dehydrogenase (LDH) play main role in the cell damage.Previous study has shown elevated level in acute leukemia and myeloproliferative disorders. Molecular markers specifically for JAK2V617F mutation in myeloproliferative disorders (MPDs) and FLT3 mutations in acute myeloid leukemia patients (AML) are well known for clinical practice. But, still only few reports have shown the correlation of LDH activity with molecular markers in Hematological Malignancy. Therefore, aim of study is to correlate LDH activity with established specific molecular markers in MPDs and AML. Methods:10 healthy individuals, 50 patients with MPDs and 50 patients with AML were included for the study. Serum LDH was performed by autoanalyser. The JAK2V617F mutations is analyse from blood by Real time PCR and FLT3 ITD/D835 mutations are analysed from blood by PCR-RFLP respectively. Statistical analysis was done by SPSS statistical software. Results: LDH Activity were higher in MPDs and AML as compared with healthy individual. Mean LDH activity was higher in FLT3 ITD/D835 positive mutation and JAK2V617F positive mutation in AML and MPDs respectively was observed. Conclusion: High LDH activity with Positive frequency of FLT3 ITD/D835 mutation in Acute Myeloid Leukemia and Positive frequency of JAK2V617F mutation in Myeloproliferative Disorders were observed.
ABSTRACT
OBJECTIVE: Assessment of the growth and nutritional status among healthy Bengali adolescent schoolgirls at peri-urban area. METHODS: In this cross sectional study, sample consists of 527 school going adolescent girls, aged 10 to 18 years. The sample of the present study was collected from a peri-urban area (Duttapukur) of north 24 parganas district of West Bengal, India. Standard anthropometric measurements including linear, curvilinear and skinfold thickness were collected from each subject. Percentile curves and nutritional indices were used to determine the growth and nutritional status respectively. RESULTS: The nature of distance curves and percentile curves of the body measurements showed a high rate of increase in 10-11 years of age group corresponding to an earlier adolescent growth spurt than average Indian girls. The adolescent girls in the current study were found to be significantly (p<0.0001) taller than Indian girls and heavier than both Indian and urban Bengali girls but slightly shorter than urban Bengali girls. They are significantly (p<0.0001) shorter and lighter than American girls, which also corresponded to 50(th) percentile curves. Age specific nutritional assessment shows different grades of malnutrition among them. Gomez's classification indicates about 60 % to 70 % adolescent girls show either moderate to mild malnutrition during their growth period. Both the indices of Waterlow's classification show least percentage of least malnutrition among the girls. CONCLUSION: The growth pattern and nutritional status observed among adolescent girls in peri-urban situation show heterogeneity with respect to some anthropometric traits and in conformity with the growth pattern and nutritional status of urban girls.