ABSTRACT
Injured/spoiled fruits like Banana, Orange, Grapes and Pomegranates were collected locally from fruit market. Prosopis juliflora pods were collected from university campus and all the fruit substrates were dried in a hot air oven at 60 0C for 8 hours. The amount of Tannin (Catechin) present in different fruit substrates were estimated by standard AOAC method. Tannins acting as anti nutrients were detoxified by using 5% Ca (OH)2 method. 100 g of each detoxified and non detoxified fruit substrate were boiled in 1 liter distilled water to obtain 10 per cent fruit extract followed by centrifugation at 5000 rpm for 5 minutes to remove sediments and impurities. 100 ml of each fruit extract medium was taken into 250 ml conical flask, sterilized by autoclaving and inoculated with 1 per cent probiotic yeast Saccharomyces cerevisiae (OBV9) and incubated. After incubation, optical density (OD660) and cell packs were determined. Based on the yield of yeast, availability of substrates and cost, among all the fruit extracts Prosopis juliflora was selected for further studies.
ABSTRACT
Proteins of the stress tolerant and mesophilic yeast were extracted using optimized protein extraction method and estimated by Bradford method. Immobilized pH gradient (IPG) strips were rehydrated with known concentrations of protein samples. Rehydrated IPG strips were run in isoelectric focusing (IEF) to separate the proteins on the basis of their pH gradient. 2DE gels were run, stained and image of the stress tolerant yeast was compared with the gel image of mesophilic yeast. The image analysis using the image master software resulted in the identification of differentially expressed spots in stress tolerant yeast. Among the differentially expressed spots, six were selected and characterized by MALDI-TOF as Enolase, Fructose bisphosphate aldolase, Alcohol dehydrogenase, 30KDa HSP, HSP70 and HSP90.
ABSTRACT
The present study evaluated the expression of hepcidin mRNA in hepatocellular carcinoma (HCC).Samples of cancerous and non-cancerous liver tissue were taken from 40 patients with HCC who underwent hepatectomy. Expression of hepcidin mRNA was evaluated by real-time PCR, and compared in tumors differing in their degree of differentiation, number of tumors, and vessel invasion. Hepcidin mRNA expression is uniformly suppressed in HCC. Hepcidin mRNA expression in noncancerous and cancerous tissues was 1991.8 (35.3–25187.4) and 62.6 (1.9–3185.8), respectively (P < 0.0001). There were no significant differences in hepcidin expression among tumors differing in their degree of differentiation, number of tumors, or vessel invasion.