Effects of Salidroside on expression of substance P and neurokinin-1 receptor of bone marrow cells in bone marrow depressed anemia mice / 中国免疫学杂志

Objective:To observe the effects of salidroside on the expression of substance P (SP)and neurokinin-1 receptor (NK-1R)of bone marrow cells(BMCs)in bone marrow(BM)depressed anemia mice,and to explore its roles for hematopoietic regulation.Methods:Automatic blood cell analysator was used to detected the changes of peripheral blood cells in each group ,immuno-histochemistry and reverse transcription-polymerase chain reaction ( RT-PCR) were used to analyze the expressions of SP and its receptor NK-1R of BMCs in each group respectively.Results: Peripheral blood testing results showed that the number of white blood cells (WBC),red blood cells(RBC)and hemoglobin(HB)of model group were significantly decreased when compared with control group.Compared with model group ,low-dose,middle-dose and high-dose salidroside obviously elevated the number of white blood cells ,and middle-dose salidroside obviously elevated the number of platelets.Immunohistochemistry studies showed that the expression of SP and its receptor NK-1R of BMCs was found in each group.Compared with control group , the expression of SP of BMCs was decreased obviously in model group(P0.05),and the expression of SP and its receptor NK-1R of BMCs was increased significantly in low-dose,middle-dose and high-dose salidroside groups (P<0.05).RT-PCR data showed that the expression of SP mRNA of BMCs was increased obviously in model group ,low-dose,middle-dose and high-dose salidroside groups when compared with control group (P<0.05).The expression of NK-1R mRNA of BMCs was un-detected in control group and model group.The expression level of NK-1R mRNA of BMCs was elevated gradually with the increase of salidroside dosage in low-dose,middle-dose and high-dose salidroside groups.Conclusion: The mRNA and protein expressions of SP and its receptor NK-1R of BMCs were up-regulated significantly by salidroside in dose-dependent manner.These data suggest that salidroside could participate in the recovery of hematopoietic function of BM depressed anemia mice by increasing the expression of SP and its receptor NK-1R of BMCs.

Effect comparison of different formulation of Dang-Gui-Bu-Xu-Tang on myelosuppression mouse

OBJECTIVE@#To compare effect of different formulation of Dang-Gui-Bu-Xu-Tang(DGBXT) on myelosuppression mouse.@*METHODS@#HPLC was used to measure active ingredients of two DGBXT formulations. Hemopoesic function of bone marrow was measured by hemopoietic progenitor cell culture and peipheral blood count. And hemopoietic factors in bone marrow were tested by ELISA.@*RESULTS@#The content level of astragaloside A in granule formulation was higher than that in decoction and the content ratio of active ingredient was close to 5 : 1. Two DGBXD formulations could significantly improve amount of peripheral blood cells, and bone marrow cells of bone marrow suppression mice (P<0.05). DGBXD granule formulation significantly increased the colony quantity of all progenitor cell lines and amount of G(2)/M and S phase cells (P<0.05). It also significantly decreased amount of G(0)/G(1) phase cells in the bone marrow and was more effective (P<0.05).@*CONCLUSIONS@#DGBXT decoction and the granule formulation all can improve the hematopoietic function of bone marrow suppression mouse. They can improve quantities of peripheral blood and nucleated bone marrow cells, and yield of the hematopoietic stem/progenitor cells in vitro colony; balance the expression of cytokine (EPO, TPO and GM-CSF) in bone marrow microenvirement. They can also facilitate hematopoietic stem/progenitor cells to enter the cell cycle. And the effect of granule formulation is more satisfactory.

Effects of salidroside on bone marrow matrix metalloproteinases of bone marrow depressed anemic mice / 生物医学工程学杂志

To examine the effect of salidroside on the expression and activities of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in bone marrow (BM) of BM depressed anemic mice by immunohistochemistry and gelatin zymography respectively, and to explore its roles in hematopoietic regulation. Immunohistochemistry showed that the expression of MMP-2 and MMP-9 of bone marrow cells (BMCs) was found in each group. Compared with control group, the expression of MMP-2 and MMP-9 was obviously increased in the model group, low-dose, middle-dose and high-dose salidroside. At day 4 after treatment of radiation and chemotherapy, the peak of the expression of MMP-2 and MMP-9 was found in middle-dose salidroside . At day 8 after treatment of radiation and chemotherapy, the peak of the expression of MMP-2 and MMP-9 was found in low-dose and middle-dose salidroside respectively. Gelatin zymography revealed that 66 kD proMMP-2, 62 kD MMP-2, 86 kD MMP-9 and 94 kD proMMP-9 were detected in control group, and the activity of MMP-9 was stronger among them. After treatment of radiation and chemotherapy, the activity of gelatinases of hemopoietic microenviroment (HM) was obviously decreased, but low-dose, middle-dose and high-dose salidroside could significantly increase the activities of proMMP-9 and MMP-9, attenuate the activity of proMMP-2. These results suggest that salidroside could promote the recovery of hematopoietic function of BM depressed anemic mice by increasing the expression and activity of MMPs, releasing the cytokines from ECM or cell membrane, repairing impaired microvessels of HM and promotion proliferation, migration and differentiation of HSCs.

Experimental research of PDE5 activity in corpora cavernosa of ganyu qizhi rat model / 中华男科学杂志

<p><b>OBJECTIVES</b>To study the PDE5 activity in corpora cavernosa of the Ganyu Qizhi model penis and the effect of the Chinese herbal medicine Shugan Liqi Huoxue (SLH) ointment on it.</p><p><b>METHODS</b>Non-injury stress stimulus method similar to human spirit stress was used to extablish the Ganyu Qizhi animal(rat) model, and the PDE5 activity in corpora cavernosa of the rat penis was measured by the method of immunohistochemistry and computer image analysis.</p><p><b>RESULTS</b>The PDE5 activity in corpora cavernosa of the high-dosage SLH group was significantly different from that of the model group (P < 0.01).</p><p><b>CONCLUSION</b>Ganyu Qizhi may increase the PDE5 activity in corpora cavernosa of the penis while SLH can reduce such activity.</p>

STUDY FOR REGULATION OF MOUSE ERYTHROPOIESIS BY SUPERNATANTS OF CULTURED PERITONEAL MACROPHAGE / 重庆医科大学学报

The effect of cultured supernatants of non-stimulated and LPS-induced peritoneal macrophages on erythropoiesis in C57BL/6J mice was studied by using the techniques of culture of hemopoietic progenitor cells in vitro. Our results indicate that(l)BFU-E growth is enhanced significantly after addition of either non-stimulated or LPS-induced supernatants of peritoneal macrophages; (2) the supernatants present positive and negative feedback control on CFU-E, growth-low concentration supernatants stimulate CFU-E and high concentration supernatants present inhibition; (3) the LPS-induced supernatants of peritoneal macrophage show stronger activities than non-stimulated supernatants to the proliteration of both BFU-E and CFU-E and(4)the supernatants of the cultured peritoneal macrophage may contain EPO-like, BPA-like stimulating activities, which regulate the proliferation of both BFU-E and CFU-E

A SIMPLE METHOD FOR MACROPHAGE ISOLATION FROM MOUSE PERITONEAL EXUDATE / 重庆医科大学学报

Macrophages are important immumo-active cells,They are integral components in the specific or nonspecific immunologic responses, We report a method for separation of macrophages using serum-coated flasks. The high viability and highly purified acmacrophages can be obtained,There are no apparent differences in basic biological activity between isolated and original macrophages. No soimulus is used in our method

THE EFFECT OF PERITONEAL MACROPHAGE CULTURE SUPERNATANTS ON MOUSE HEMOPOIESIS / 解剖学报

BFU-E growth is enhanced significantly after addition of either non-stimulated or LPS-stimulated peritoneal macrophage culture supernatants. This supernatant shows positive and negative control on the growth of CFU-E and CFU-GM, low concentration supernatant shows promoting activity and high concentration supernatant presents inhibition. The LPS-stimulated supernatant shows much stronger promoting activities than non-stimulated supernatant. This supernatant has no obviously action on the proliferation of CFU-S. The mechanism of the regulation of mouse hemopoiesis by the conditioned media of the peritoneal macrophage is discussed. Our results show that this supernatant of cultured peritoneal macrophages may contain EPO-like, BPA-like and CSF-GM-like factors.