ABSTRACT
Objective:To investigate whether chitosan-polyelectrolyte complex (CS-PEC) can be used as scaffold for chondrocyte culture and for cartilage regeneration in vivo.Methods:Condylar chondrocytes of fetal mouse were seeded onto the three-dimension gel scaffolds of CS-PEC and cultured.The cultured chondrocytes/CS-PEC complex samples were transplanted subcutaneously into nude mice and the CS-PEC scaffold without chondrocyte was used as the control.The animals were sacrificed 4 and 8 weeks after operation respectively.Cartilage formulation was observed by histological and immunohistochemical methods.Results:In the in vitro culture the majority of cells attached to the CS-PEC surface and expanded rapidly. 4 weeks after transplantation,in the chondrocytes/CS-PEC complex the scaffold maintained mostly the original structure. Hypertrophic chondrocytes appeared in scaffold materials. CollagenⅡwas positive in the new cartilage. 8 weeks after transplantation the scaffold degraded almost completely and new cartilage could be observed. CollagenⅡ and cartilage matrix was positive in the new cartilage and the collagen I was positive in the surrounding fibroblast-like cells. In control transplants,8 week after transplantation some fibre-like tissue formed in the circumference, but there was no new cartilage formation and the collagen II and the cartilage matrix was negative.Conclusions:CS-PEC may be used as scaffold for fibre-cartilage regeneration.
ABSTRACT
Objective:To study the mandibular movement characteristics of subjects with severe tooth wear (TW).Methods:30 TW patients (24 men, 6 women, mean age 51 years) were served as TW group and 10 subjects with individual normal occlusion were served as control group (7 men, 3 women, mean age 40.5 years). All cases were examined by means of K6-1 Evaluation System manufactured by Myotronics US. The traces of the mandibular opening-closing movement and border movement were recorded.Results:During the movement from mandible posture position(MPP) to intercuspal position(ICP), the TW patients showed unstable characteristic. The velocity showed no significant difference between the two groups during opening and closing, but the congruence and smoothness of traces during the mandibular opening-closing and border movements in the TW patients were a little worse than the controls; and the TW patients had less mandibular movement index(MMI) than the controls(P