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Objective:To compare the clinical efficacy of drug coated balloon (DCB) vs. plain old balloon (POB) on in-stent restenosis (ISR) of femoropopliteal artery occlusive disease of the lower limb. Methods:The clinical data of 91 ISR patients admitted at Shanxi Bethune Hospital from Jul 2016 to Dec 2017 were retrospectively analyzed. The primary patency rates were compared.Results:There were 43 patients treated with drug coated balloons and 48 patients treated with plain old balloons. The surgical procedure was successful in all cases, and the symptoms of lower limb ischemia were significantly improved after surgical procedure. The primary patency rate of patients who were treated by drug coated balloons was significantly higher than by plain old balloons at 12 months after surgery (83.7% vs. 62.5%, P<0.05). Conclusion:The use of drug coated balloons could acquire more satisfactory short-term clinical efficacy for ISR patients of femoropopliteal artery occlusive disease.
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Vagus nerve reflex is a rare complication of percutaneous renal decompression. It is often induced by excessively rapid decompression of severe hydronephrosis and traction of the main nerves innervating the kidney. The clinical manifestations are irritability, sweating, clammy skin, hiccups, slow heart rate. It is easy to misdiagnose. In this study, 4 patients with vagus nerve excitement after percutaneous renal decompression were treated. After monitoring the patient’s vital signs and giving treatment such as expanding blood volume and raising blood pressure, the symptoms gradually disappeared.
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The metastasis of renal cell carcinoma to the ureter is a rare phenomenon, and synchronal detection of metastasis to the contralateral ureter is a rarer phenomenon. A 62-year-old male patient with painless hematuria was examined and detected a renal cell carcinoma on the right kidney and bleeding from the left ureter. Ureteroscopy revealed a tumor in the left upper ureter, and biopsy suggested clear cell carcinoma. Laparoscopic radical nephrectomy was performed to resect the right renal cell carcinoma, and the pathology revealed a clear cell carcinoma, with Fuhrman nuclear grade 2 class. The ureteral tumor was resected 3 months later and the pathology revealed renal clear cell carcinoma. Sunitinib was used for 37 months, and there was no tumor recurrence or metastasis so far.
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Objective:To analyze the clinical features, treatment, and pregnancy outcomes of women with venous thromboembolism (VTE) during pregnancy and puerperal period.Methods:Clinical data of 81 patients with gestational and puerperal VTE admitted to the Department of Vascular Surgery of Dayi Hospital Affiliated to Shanxi Medical University from January 2014 to December 2017 were retrospectively analyzed. Rank-sum test, t-test or Chi-square test (or Fisher's exact test) were used for statistical analysis. Results:(1) The average age of these women was (30.9±5.2) years old and 20 (24.7%) were ≥35 years old. Pregnant women accounted for 50.6%(41/81). Forty cases were in the puerperium, of which 34 developed VTE within 30 days after delivery. (2) The thrombus was predominantly peripheral type in all subjects after excluding five patients with isolated pulmonary thromboembolism (PTE) (61.8%, 47/76), especially in pregnant patients (80.0%, 32/40), while mixed type was often occurred in the puerperium (44.4%, 16/36, χ2=13.417). The initial symptoms and signs were mainly swelling (43.4%, 33/76) and pain (28.9%, 22/76) with acute onset. Thrombi were mainly found in the deep veins of lower extremities (74.1%, 60/81). More pregnant patients had deep venous thrombosis (DVT) in the lower limbs (87.8%, 36/41), while DVT in the puerperium was often complicated by PTE (30.0%, 12/40, χ2=7.961). Intermuscular veins were the most common affected veins (72.4%, 55/76), especially during pregnancy (85.0%, 34/40), while for those cases during puerperium, multiple veins, such as intermuscular (58.3%, 21/36), femoral (58.3%, 21/36), popliteal (50.0%, 18/36), iliac (47.2%, 17/36), tibial (44.4%, 16/36) and fibular vein (38.8%, 14/36) were involved. (3) The highest risk factor was age ≥35 years old (24.7%, 20/81), followed by antithrombin deficiency (23.5%, 19/81) and hypertensive disorders of pregnancy (HDP, 21.0%, 17/81). There were no significant differences in the distribution of different risk factors between patients during pregnancy and puerperium. D-dimer levels were higher in puerperal patients than in pregnant women [1 029 (639-1 778) vs 619 (355-1 117) μg/L, Z=-3.336, P<0.001]. (4) All patients received systemic anticoagulant therapy. Twenty-four patients (29.6%, including five in pregnancy and 19 in puerperium) were treated with thrombolytic therapy due to extensive thrombus formation. The average hospital stay was (7.3±2.1) d. (5) Among the 41 patients developing VTE during pregnancy, nine (21.9%) received induced labor; 26 (63.4%) underwent cesarean section; six (14.6%) delivered vaginally. Among the 32 pregnancies continuing to deliver, eight (25.0%) premature infants and one (3.1%) macrosomia were born. Conclusions:Maternal VTE mainly occurs in the third trimester or within 30 days after birth. VTE in the puerperal period commonly involves multiple veins and is characterized by extensive thrombus formation. Pregnant women aged ≥35 years, with antithrombin deficiency or complicated with HDP, should be closely observed to prevent VTE as it may affect pregnancy outcomes.
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Objective To explore the feasibility of the modified RUSS (Resorlu-Unsal Stone Score)renal stone scoring system in predicting the stone free rate after Flexible Ureteroscopic Lithotripsy(FURL).Methods Retrospective analysis was performed on 104 cases of renal stones treated by FURL from March 2017 to January 2019 in the second affiliated hospital of zhengzhou university,including 75 males and 29 females.Age 20-75 (47.79 ± 13.21) years.BMI 18.2-38.3 (24.4 ± 3.7) kg/m2;Crushed stone site:left 56 cases,right 48 cases.There were 23 cases of renal inferior calyces calculi,81 cases of non-inferior calyces calculi,and 19 cases of renal inferior calyces calculi with IPA ＜ 45°;American Society of Anesthesiology (ASA) scores:65 cases of grade Ⅰ,39 cases of grade Ⅱ;There were 71 patients with urinary tract infection before operation.There were 6 cases of renal puncture fistula before operation.Preoperative indwelling ureteral stent in 26 cases;There were 32 cases with history of extracorporeal shock wave lithotripsy.There were 27 patients with a history of urolithiasis therapy.The same physician used preoperative urinary CT + 3D reconstruction imaging data to measure the factors influencing the postoperative stone free rate.RUSS renal stone scoring system was used to score the stones of patients before operation,and the relationship between the scores and the stones free rate was analyzed.The RUSS renal stone scoring system was supplemented and improved by including staghorn stone,duplicate renal,caliceal diverticulum,renal malrotation,stone area,and CT value related indexes.The modified RUSS renal stone scoring system was used to score the preoperative stone condition of patients,and the relationship between the score and the stone free rate was analyzed.The receiver operating characteristic curve (ROC) was drawn and the area under the curve (AUC) was calculated to compare the advantages and disadvantages of the modified RUSS,the characteristics of the stones,and RUSS system.Results The stone free rate was 69.2％ (72/104) 4 weeks after the operation,and there were no postoperative complications.Single-factor analysis showed that stone area,CT value,number of renal calyx involved by stone,multiple stones,IPA,stone size grading,renal anatomic structure abnormality,staghorn stone were all related risk factors affecting postoperative patients.Multi-factor analysis showed that stone area,number of renal calyces involved by stone,multiple stones,IPA and stone size were independent risk factors affecting the stone free rate after FURL.RUSS scores ranged from 0 to 3 points,corresponding to stone clearing rates of 86.8％ (33/38),67.7％ (23/34),58.3％ (14/24) and 25.0％ (2/8),respectively.Stone clearing rates were significantly correlated with stone grading (P ＜ 0.05).The total scores of stone free group and residual stone group were (0.87 ± 0.96) points and (1.53 ± 0.98) points,respectively,with significant differences between the groups (P ＜ 0.05).The range of modified RUSS score was 0-4 points,corresponding to the stone clearing rate of 100.0％ (25/25),92.3％ (24/26),54.2％ (13/24),47.4％ (9/19),and 10.0％ (1/10),respectively.Stone free rate was significantly correlated with stone grading (P ＜ 0.05).The total scores of stone free group and residual stone group were (1.15 ± 1.13) points and (2.81 ± 0.93) points,respectively,with significant differences between the groups (P ＜ 0.05).The AUC of stone features was smaller than that of the modified RUSS scoring system.The AUC of RUSS scoring system (0.707,95 ％ CI 0.598-0.815) was smaller than that of the modified RUSS scoring system (0.865,95％ CI 0.797-0.933).Conclusions Both the RUSS scoring system and the modified RUSS scoring system could accurately predict the postoperative stone free status of FURL,while the modified RUSS scoring was more accurate than the RUSS scoring.
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Objective@#To explore the feasibility of the modified RUSS (Resorlu-Unsal Stone Score) renal stone scoring system in predicting the stone free rate after Flexible Ureteroscopic Lithotripsy(FURL).@*Methods@#Retrospective analysis was performed on 104 cases of renal stones treated by FURL from March 2017 to January 2019 in the second affiliated hospital of zhengzhou university, including 75 males and 29 females. Age 20-75 (47.79±13.21) years. BMI 18.2-38.3 (24.4±3.7) kg/m2; Crushed stone site: left 56 cases, right 48 cases. There were 23 cases of renal inferior calyces calculi, 81 cases of non-inferior calyces calculi, and 19 cases of renal inferior calyces calculi with IPA<45°; American Society of Anesthesiology (ASA) scores: 65 cases of grade Ⅰ, 39 cases of grade Ⅱ; There were 71 patients with urinary tract infection before operation. There were 6 cases of renal puncture fistula before operation. Preoperative indwelling ureteral stent in 26 cases; There were 32 cases with history of extracorporeal shock wave lithotripsy. There were 27 patients with a history of urolithiasis therapy. The same physician used preoperative urinary CT+ 3D reconstruction imaging data to measure the factors influencing the postoperative stone free rate. RUSS renal stone scoring system was used to score the stones of patients before operation, and the relationship between the scores and the stones free rate was analyzed. The RUSS renal stone scoring system was supplemented and improved by including staghorn stone, duplicate renal, caliceal diverticulum, renal malrotation, stone area, and CT value related indexes. The modified RUSS renal stone scoring system was used to score the preoperative stone condition of patients, and the relationship between the score and the stone free rate was analyzed. The receiver operating characteristic curve (ROC) was drawn and the area under the curve (AUC) was calculated to compare the advantages and disadvantages of the modified RUSS, the characteristics of the stones, and RUSS system.@*Results@#The stone free rate was 69.2% (72/104) 4 weeks after the operation, and there were no postoperative complications. Single-factor analysis showed that stone area, CT value, number of renal calyx involved by stone, multiple stones, IPA, stone size grading, renal anatomic structure abnormality, staghorn stone were all related risk factors affecting postoperative patients. Multi-factor analysis showed that stone area, number of renal calyces involved by stone, multiple stones, IPA and stone size were independent risk factors affecting the stone free rate after FURL. RUSS scores ranged from 0 to 3 points, corresponding to stone clearing rates of 86.8% (33/38), 67.7% (23/34), 58.3% (14/24) and 25.0% (2/8), respectively. Stone clearing rates were significantly correlated with stone grading (P<0.05). The total scores of stone free group and residual stone group were (0.87±0.96) points and (1.53±0.98) points, respectively, with significant differences between the groups (P<0.05). The range of modified RUSS score was 0-4 points, corresponding to the stone clearing rate of 100.0% (25/25), 92.3% (24/26), 54.2% (13/24), 47.4% (9/19), and 10.0% (1/10), respectively. Stone free rate was significantly correlated with stone grading (P<0.05). The total scores of stone free group and residual stone group were (1.15±1.13) points and (2.81±0.93) points, respectively, with significant differences between the groups (P<0.05). The AUC of stone features was smaller than that of the modified RUSS scoring system. The AUC of RUSS scoring system (0.707, 95%CI 0.598-0.815) was smaller than that of the modified RUSS scoring system (0.865, 95%CI 0.797-0.933).@*Conclusions@#Both the RUSS scoring system and the modified RUSS scoring system could accurately predict the postoperative stone free status of FURL, while the modified RUSS scoring was more accurate than the RUSS scoring.
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Objective To explore the best intervention based on evidences of enteral nutrition support complicated with diarrhea in critically ill patients and evaluate its clinical effects.Methods Evidence was obtained using Evidence-based Nursing,and was implemented in clinical practice after localization,we formulated numing recommendations for regulating nurses' clinical practice regarding enteral nutrition support complicated with diarrhea in ICU patients;we conducted multiple online and offline training for nurses.The rate of diarrhea in ICU patients undergoing enteral nutrition support before and after using best evidence was compared,and awareness and implementation of best evidence among nurses before and after training were also compared.Results After adapting the best evidence,the rate of enteral nutrition associated diarrhea decreased from 25.58％ to 13.89％;ICU nurses' diarrhea identification and evaluation,analysis of influencing factors of enteral nutrition associated diarrhea,selection of enteral nutrition formula,nasal feeding,etc.,were significantly improved (P＜0.05);there was no significant difference in enteral nutrition infusion,and nutrition preparation and preservation (P＞0.05);rare of implementation of best evidence in ICU nurses was greater than 80％.Conclusion Evidence-based practice can effectively prevent enteral nutrition associated diarrhea in ICU patients.Nurses can solve enteral nutrition associated diarrhea using scientific nursing methods through implementation of best evidence,in order to nursing quality.
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Enterovirus 71 is a neuroinvasive virus that is associated with severe neurological complications. We had earlier suggested that the replication capacity of a severe strain was higher than that of a mild strain. The recombinant 3CRV and 3CDRV virus strains were successfully rescued in our previous study. In the present study, we found no difference in virulence between 3CRV and severe strains. However, the capacity of replication and to cause cell injury of 3CDRV strain decreased in vitro, especially at 39.5 °C. Replacement of 3CD region in the severe strain led to milder symptoms, less body weight loss, and lower viral load in ICR mice. Histopathological findings indicated less severe injury in mice infected with 3CDRV strain. This study suggests that the 3CD region contributes to the attenuation of the severe strain, including its replication capacity and temperature sensitivity.
Subject(s)
Animals , Mice , Cytopathogenic Effect, Viral , Enterovirus A, Human , Genetics , Virulence , Enterovirus Infections , Pathology , Virology , Gene Expression Regulation, Viral , Mice, Inbred ICR , Mutation , Viral Load , Viral Proteins , Genetics , Metabolism , Virulence , Virus ReplicationABSTRACT
Objective To understand the status of implementation of invasive ventilator circuit changes in ICU nurses at the 3A general hospitals in Shanxi Province, and mastering and demand of related knowledge of ICU nurses, and by this discuss the possible causes of execution inconsistency in invasive ventilator circuit changes interval so as to provide a clear basis for the specification and circuit changes. Methods After a review of relevant literature at home and abroad as well as expert consultation, a self-designed questionnaire was established, take two ways of on-site issuance and mailing, ICU nurses from 13 hospitals were selected randomly to investigate about the invasive circuit changes interval in Shanxi Province. Results A total of 724 nurses from 34 ICU of 13 hospitals were surveyed. A unified circuit changes interval of ICU accounted for 73.5% (527/717). ICU nurses currently provisions and practical implementation of invasive ventilator circuit changes interval tend to 7 d. Different ICU provisions and ICU nurses actual implementation of circuit change were significantly different (χ2=24.839, 35.760, P < 0.01). Conclusions Hospitals should choose the right way to strengthen the ICU nurses invasive ventilator circuit changes training interval and knowledge, to develop the term for their own security environment, thereby reduce the workload of nurses, reduce medical costs and improve care service quality.
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Objective To implore the characteristics of epithelial-mesenchymal transition(EMT) in human prostate cancer stem progenitor (S/P) cells isolated from LNCaP cell lines.Methods The S/P cells were obtained through florescence-activated cell sorting (FACS).Western blot and immunofluorescence assay were used to detect the S/P cells' EMT markers expression,such as E Cadherin,N Cadherin,Vimentin and Snail.Soft agar assay was used to detect the tumorigenesis ability of S/P cells.Cell migration assay was used to detect the migration ability of S/P cells.Results Compared with non S/P cells,the expressions of EMT markers,such as N Cadherin,Vimentin and Snail,were increased in S/P cells,while the expressions of epithelial marker and E Cadherin were decreased in S/P cells.After cultured for three weeks,S/P cells and non S/P cells both clonally grew.The colony numbers were (18.34±1.21) and (82.27±7.54),respectively (t =8.617,P =0.001).After cultured for 48 hours,the migration cells number was (25.33±5.13) in non S/P cells and (74.33±7.64) in S/P cells (t =7.953,P =O.001).Conclusions Human prostate cancer S/P cells isolated from LNCaP cell line have some characteristics of EMT,such as stronger tumorigenesis and migration ability,which could promote tumor invasion and metastasis.
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Objective To evaluate the mid-term and long-term result of mechanical aspiration thrombectomy or combined with balloon thrombectomy,venoplasty and catheter-directed thrombolysis therapy for lower extremity deep venous thrombosis.Methods Clinical data of 1 068 admitted patients were analysed retrospectively from January 2000 to June 2010.Among these patients,271 cases were treated by thrombectomy only,537 cases received thrombectomy with arterial catheter-directed thrombolysis,260 cases were treated by thrombectomy with dorsalis pedis vein thrombolysis,492 patients with stenosis or obstruction of iliac vein underwent venoplasty treatment.Results After 36 month follow-up edema,pigmentation and chronic ulcers were 10.26％,7.28％,1.55％ respectively.The patency without reflux,patency with reflux,residual thrombus,occlusion rate of the iliofemoral vein were 84.25％,8.95％,4.3％,2.5％ respectively; The patency,residual thrombus and occlusion rate of popliteal vein were 93.32％,5.49％,1.19％.Iliac vein stent patency rate was 92.28％.Conclusions Endovascular treatment for lower extremity deep venous thrombosis was a reasonable therapy with a satisfactory mid-term and long-term result,protecting venous valve function and reducing the morbidity of post-thrombotic syndrome.
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<p><b>OBJECTIVE</b>To explore the anesthetic effect of preemptive analgesia of frequency acupoint electrical stimulation on painless-induced abortion as well as its effect on anesthetics dosage.</p><p><b>METHODS</b>Ninety cases of early pregnancy who selected painless-induced abortion were randomly divided into two groups, 45 cases in each group. Frequency acupoint electrical stimulation at Ciliao (BL 32) and Shenshu (BL 23), disperse-densewave, 2 Hz/100 Hz in frequency for 15 to 20 min, was applied in the group A, which was followed by intravenous anesthesia of propofol. The intravenous anesthesia of propofol was applied in the group B. The blood pressure (BP), heart rate (HR) and SpO2 before, during and after surgery, anesthetic effect and dosage, waking time and adverse events were observed in the two groups.</p><p><b>RESULTS</b>The BP and HR during and after the surgery in the group A were not statistically different from those before the surgery (all P > 0.05). The BP was reduced and HR was slowed down during the surgery in the group B, which was significantly different from those before the surgery as well as those in the group A (all P < 0.05). The dosage of propofol was (114. 3-+6. 1) mg in the group A. obviously less than (193.2 +/- 8.9) mg in the group B (P < 0.05). The waking time was (5.6 +/- 1.2) min in the group A, obviously less than (10.1 +/- 3.9) min in the group B (P < 0.05). As for anesthetic effect, the incidence of Grade I in the group A was more than the group B (P < 0.05). The adverse events, including nausea, vomiting and contractions pain in the group A were evidently less than those in the group B (all P < 0.05).</p><p><b>CONCLUSION</b>The preemptive analgesia of frequency acupoint electrical stimulation could significantly improve anesthetic effect of painless-induced abortion, reduce dosage of anesthetics, shorten waking time of surgery and guarantee the safety of surgery.</p>
Subject(s)
Adult , Female , Humans , Pregnancy , Young Adult , Abortion, Induced , Acupuncture Analgesia , Acupuncture Points , Electric Stimulation , Pain ManagementABSTRACT
Objective To observe the curative effect of external physical vibration lithecbole (EPVL) therapy after extracorporeal shock-wave lithotripsy (ESWL) in upper urinary calculi.Methods A total of 133 patients of upper urinary calculi with randomly divided into 2 groups after ESWL therapy during the period of 2012 October to 2013 February.The EPVL group (66 cases) used the physical vibration lithecbole treatment,and the natural lithecbole group (67 cases) used the method by drinking water,adding exercise and other natural lithecbole method.Of the EPVL group,6 of the stones were located in the upper or middle calyx,13 in renal pelvis,16 in lower renal calyx and 31 in ureter,the stone diameter was 10-15 mm.Of the natural lithecbole group,8 of the stones were located in the upper or middle calyx,17 in renal pelvis,15 in lower renal calyx and 27 in ureter,the stone diameter was 10-15 mm.The clinical data of the curative effect,side-effect,and complications were collected and analyzed systematically.Results In the EPVL group,the average times of lithagogue treatment was 2.6 times,51 patients (77％) expelled stones on the day of lithecbole,and the stone free rate in a week was 79％ (52/66).Especially,81％ (13/16) of the lower renal calyx expelled stones on the day of lithecbole,and the stone free rate in a week was 88％ (14/16).Whereas,30 patients (45％) in the natural lithecbole group expelled stones on the day of ESWL,and the stone free rate in a week was 49％ (33/67) ; the lower renal calyx stone expulsion rate on the day of ESWL was 33％ (5/15),and the stone free rate in a week was 40％ (6/15).The curative effects were significantly different between the 2 groups (P＜ 0.05).There were no serious complications in both groups.Conclusions EPVL machine can significantly promote the stone expulsion after ESWL.Compared with traditional methods,EPVL therapy has a better curative effect,especially on lower renal calyx stones.EPVL is a safe and noninvasive treatment.
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Objective To evaluate the clinical value of determination of composition of kidney stones by shap and density and of prediction of the efficacy of extracorporeal shock-wave lithotripsy (ESWL) by X-rays. Methods The data of 358 patients were analyzed,with 204 male and 154 female and with 276 cases of solitary stones and 82 cases of multiple stones.Determine the composition of kidney stones by shap and density,predict the efficacy of ESWL by X-rays,and choose the appropriate method of treatment.Analyze stone chemical property by Infrared stone composition automatic analyzer to checkout the prediction results before surgeries. Results 339 cases were successful to remove stones after treatment.The prediction results of 308 cases (86.0％) were consistent in stone chemical property,and the preperative prediction results of 339 cases (94.7％) were consistent in ESWL efficacy. Conclusions Determination of composition of kidney stones and prediction of the efficacy of ESWL by X-rays were feasible.
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Objective To explore the way to reduce the absorption of irrigating fluid during PCNL. Methods The amount of absorbed fluid during PCNL in 20 cases (13 males and 7 females)was monitored by ethanol method. The average age was 40 years old. The maximum diameter of calculi by average was 25 mm. Heart rate and mean arterial pressure were monitored during operation.Haemoglobin concentration, serum Na+, K+, Cl-, carbon dioxide-combining power and serum creatinine were measured before and after operation. The amount of irrigating fluid, the duration of operation, and the intrapelvic pressure were recorded. Results The absorbed volume of irrigating fluid in 20 cases was from 50. 2 to 685.0 ml. There was no significant difference in heart rate, mean arterial pressure, serum Na+ , K+ , Cl- and serum creatinine between pre-operation and post-operation(P>0.05), but the postoperative haemoglobin concentration (130.4±16.3 g/L) and carbon dioxide-combining power (24.1±3.2 mmol/L) were lower than that before operation( 142.6±15.6 g/L,26.4±2.0 mmol/L), the differences were significant (P<0. 05). The mean volume of absorbed irrigating fluid in patients with the cumutative time of the intrapelvic pressure higher than 30 mm Hg >10 min,the duration of operation >1 h, or the amount of irrigating fluid >10000 ml was 381. 1, 301.6,261.6 ml respectively, which was higher than that in the other cases (142.9, 136.4, 130.2 mi), the differences were significant (P<0.05). Conclusions The ethanol method is simple, convinent,safe, valid, and is suitable for patients with compromised cardiorespiratory or renal function, who are more likely to develop volume overload because of fluid absorption.
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<p><b>OBJECTIVE</b>To investigate the changes of oval cell proliferation rate in the rat 2-acetylaminofluorene/partial hepatectomy (2-AAF/PH) model.</p><p><b>METHODS</b>Livers were collected from 2-AAF/PH rats at different time points after hepatectomy. Paraffin sections were investigated by double immunofluorescent staining with confocal microscopy for oval cell marker epithelial cell adhesion molecule and proliferative index proliferating cell nuclear antigen, or epithelial cell adhesion molecule and alpha-smooth muscle actin. Deposition of matrix in liver tissue was detected by sirius red staining.</p><p><b>RESULTS</b>Response of ductular oval cells could be observed in portal area at 2 days after PH, and the number of oval cells reached its peak at 9 days and then gradually declined. Oval cell proliferation rate decreased from (91.3 +/- 1.6)% at 2 days after PH to (53.6 +/- 4.4)% at 12 days (P < 0.01). In addition, oval cells infiltrating into liver parenchyma were closely associated with activated hepatic stellate cells and extracellular matrix.</p><p><b>CONCLUSIONS</b>Oval cell proliferation rate starts decreasing before its number reaches a peak in 2-AAF/PH model. Hepatic stellate cells probably tightly regulate oval cell number through secreting several factors and producing extracellular matrix.</p>
Subject(s)
Animals , Male , Rats , 2-Acetylaminofluorene , Pharmacology , Cell Division , Cell Proliferation , Hepatectomy , Liver , Cell Biology , Rats, Sprague-Dawley , Stem Cells , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To study the molecular mechanism of TAp63gamma-induced cell apoptosis.</p><p><b>METHODS</b>Transcription and protein expression of apoptosis inducing factor and p63 were investigated by immunohistochemistry and RT-PCR in human esophageal squamous carcinoma cell line EC9706 respectively. Twenty-four hours after transfection with pcDNA3.1-TAp63gamma, the apoptosis and translocation of apoptosis inducing factor in EC9706 cells were studied by flow cytometry, laser confocal microscopy and mitochondrial/cytosol/nuclear extraction analysis respectively. Down-regulation of apoptosis inducing factor protein was achieved by RNAi and pretreatment with caspase inhibitor zVAD.fmk of EC9706 cells.</p><p><b>RESULTS</b>Presence of protein expressions of apoptosis inducing factor and absence of TAp63gamma was observed in the cytoplasm of untransfected cells. RT-PCR verified the subtype of p63 in EC9706 cells was DeltaNp63. After 24 hours of transfection, both nuclear and cytoplasmic expression of apoptosis inducing factor protein were observed in cells transfected with TAp63gamma and p53 expression vectors, but not in cells transfected with control vector. Cell apoptosis rates were 1.37%, 13.64%, 4.52%, 4.03% and 1.91% in the pcDNA3.1 transfection group, pcDNA3.1-TAp63gamma transfection group, apoptosis inducing factor siRNA and pcDNA3.1-TAp63gamma transfection group, zVAD.fmk treatment group, and the group receiving apoptosis inducing factor siRNA, plus zVAD.fmk treatment and pcDNA3.1-TAp63gamma transfection, respectively.</p><p><b>CONCLUSIONS</b>Apoptosis inducing factor of EC9706 cells is released from mitochondria into both the cytoplasm and nucleus during TAp63gamma induced apoptosis. Down-regulation of apoptosis inducing factor inhibits TAp63gamma-induced apoptosis. Overall, TAp63gamma-induced apoptosis is dependent on the expression of apoptosis inducing factor and caspase.</p>
Subject(s)
Humans , Amino Acid Chloromethyl Ketones , Pharmacology , Apoptosis , Apoptosis Inducing Factor , Genetics , Metabolism , Carcinoma, Squamous Cell , Metabolism , Pathology , Caspase Inhibitors , Cell Line, Tumor , Cell Nucleus , Metabolism , Cytoplasm , Metabolism , Down-Regulation , Esophageal Neoplasms , Metabolism , Pathology , Mitochondria , Metabolism , Plasmids , Protein Transport , RNA Interference , RNA, Small Interfering , Genetics , Trans-Activators , Genetics , Metabolism , Transcription Factors , Transfection , Tumor Suppressor Proteins , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the change of paeonol and paeoniflorin, the two major active ingredients contained in Cortex Moutan cultivated in Dianjiang county of Chongqing, due to the change of some influence factors, and explore suitable plant conditions and quality cotrol methods of Cortex Moutan.</p><p><b>METHOD</b>Paeonol and paeoniflorin were determined by HPLC in samples from Dianjiang.</p><p><b>RESULT AND CONCLUSION</b>The ratio of paeonol and paeoniflorin in Cortex Moutan was regularly influenced by altitude, the growth years and harvest time. Cortex could be cultivated at altitude of 400 m to 800 m but 600 m is the best aria because of the peak of the percentage composition of paeonol and paeoniflorin at 600 m. The first and middle third of October in the fifth year is the best picking time of Cortex Moutan because of the maximum of the percentage composition of paeonol and paeoniflorin.</p>
Subject(s)
Acetophenones , Altitude , Benzoates , Bridged-Ring Compounds , China , Drugs, Chinese Herbal , Chemistry , Glucosides , Monoterpenes , Paeonia , Chemistry , Plant Bark , Chemistry , Plants, Medicinal , Chemistry , Quality Control , Seasons , SoilABSTRACT
The study was aimed to investigate the factor V coagulation activity (FV:C), and to evaluate FVgene polymorphisms and activated protein C resistance (APCR) in the patients with venous thromboembolism (VTE). 95 patients with VTE and 95 normal controls were investigated for FV gene polymorphisms. FV Leiden, FVCambridge, and FVHong Kong were detected by PCR, MnlI and BstNI digestion respectively. FVAsp79His and FVI359T were detected by MassARRAY. FV:C and APCR in 65 patients with VTE and 60 normal controls were determined by a one-stage clotting method and the APTT-based assays respectively. The results showed that the mean levels of plasma FV:C were significantly higher in VTE group than that in controls (108.03% +/- 28.29% vs 95.17% +/- 29.75%) (P = 0.008), the incidence of APCR were 20.0% (13 of 65 cases) in patients with VTE and 5.0% (3 of 60 cases) in normal controls (P = 0.012). FV Leiden, FVCambridge, FVHong Kong, FVAsp79His and FVI359T mutations were not found in two groups. It is concluded that the increased plasma level of FV:C is a risk factor for VTE. There is APCR in both groups, APCR is also a risk factor to VTE. APCR may not be associated with mutations of FV Leiden, FVCambridge, FVHong Kong, FVAsp79His and FV I359T polymorphisms, other factors need to study further in APCR.
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Activated Protein C Resistance , Genetics , Factor V , Genetics , Metabolism , Polymorphism, Genetic , Protein C , Metabolism , Venous Thromboembolism , Blood , GeneticsABSTRACT
Objective To investigate the diagnostic points of multiple sclerosis and multiple metastatic encephaloma.Methods Choose a case of multiple sclerosis,which had been misdiagnosed as multiple metastatic encephaloma and operated on,to analyse its reasons of misdiagnosis.Results Reasons for the misdiagnosis:The neglect of the importance of the disease history.The scant knowledge of the early represen- tations of the atypical MS(demyelinating pseudotumor).The lack of the knowledge of the non-demyelinating lesions of the MS.The lack of attention to the different representations of the MRI scan between the acute MS and the multiple metastatic encephaloma.The excessive importance is attached to the imageological report. The neglect of the routine examination of cerebrospinal fluid.Conclusion When multiple metastatic en- cephaloma develops,the differential diagnosis of MS should be made.The further examinations should be done to those cases which are hard to tell the difference with metastatic encephaloma by clinical and imageo- logical representations.Try to avoid undergoing surgery or radiotherapy hastily.