ABSTRACT
The aim of the current study was to evaluate the antinociceptive activity of the selected Pakistani medicinal plants [Chenopodium botrys, Micromeria biflora and Teucrium stocksianum] in-vivo followed by their antioxidant potential against 1,1-diphenyl-2-picrylhidrazyl [DPPH] in-vitro. The results demonstrated profound antinociceptive effect of both the crude methanolic extract of Chenopodium botrys [CBM] and subsequent aqueous fraction [CBW] of C. botrys with 80.76% and 84% pain relief in acetic acid induced writhing test at 100 mg/kg i.p respectively. Similarly the crude methanolic extract of Micromeria biflora [MBM] and its subsequent aqueous fraction [MBW] with 66.46% 78.08% pain reversal in acetic acid induced writhing test respectively at 100mg/kg i.p. However, the crude methanolic extract and isolated water fraction of Teucrium stocksianum [TS] did not show any significant effect at test doses. Both the crude extracts and aqueous fractions of selected medicinal plants exhibited marked scavenging effects on DPPH and therefore strongly support the antinociceptive activity. Phytochemical analysis indicated the presence of various classes of natural products [alkaloids, terpenoids, flavonoids etc.] and thus the current finding can be attributed to the presence of these compounds. In short, our findings provide a strong scientific background to the folk uses C. botrys and M. biflora in the management of various painful conditions
ABSTRACT
OBJECTIVE: To evaluate the sedative effect of Diospyros lotus L (D. lotus) extract in mice using the open field and Rota rod tests. METHODS: For the sedative and muscle relaxants activities of extract/fractions of the plant, in-vivo open field and phenobarbitone-induced sleeping time were used, while the Roda rod test was employed in animals for the assessment of muscle relaxant activity. RESULTS: Results from this investigation revealed that the extracts of D. lotus have exhibited significant sedative effect in mice (45.98%) at 100 mg/kg i.p. When the extract was partitioned with different solvents, the n-hexane fraction was inactive whereas the chloroform fraction was the most active with 82.67% sedative effect at 50 and 100 mg/kg i.p. On the other hand, the ethyl acetate and n-butanol fractions displayed significant sedative effects (55.65% and 40.87%, respectively) at 100 mg/kg i.p. Among the tested extract/fractions, only chloroform and ethyl acetate fractions showed significant (P < 0.05) muscle relaxant activity in the Rota rod test. CONCLUSIONS: In short, our study provided scientific background to the traditional uses of D. lotus as sedative.