ABSTRACT
·AIM:To analyze the difference in the level of fat-specific phospholipase A2 ( AdPLA) mRNA in the orbital adipose tissue between patients with thyroid - associated ophthalmopathy (TAO) and normal subjects. ·METHODS: Thirty-seven patients ( 37 eyes) with TAO ( stationary stage Ⅲ ) who underwent orbital decompression surgery in our hospital from December 2016 to December 2017 were selected as the observation group; in the same period, 35 cases (35 eyes) of normal orbital adipose tissue were selected as the control group, which the source of the normal eyelid tissue was cosmetic surgery, pouch resection and ptosis correction. The eyeball protrusion was used to measure the degree of protrusion of the eye on the fat side of the observation group and the control group, and the BMI status of the two groups was calculated. A 16-slice spiral CT from Siemens motion was used to perform CT examination of the eyelids of both groups of subjects. Image J was used to detect the fat volume of the fat side and real time PCR was used to detect the expression of AdPLA mRNA in the adipose tissue of the eyelids. ·RESULTS: There was no significant difference in mean age, BMI, and gender between the observation group and the control group (P>0. 05); the intraocular fatty acid content, ocular outgrowth and intracellular AdPLA mRNA expression in the observation group were 32. 21 ± 1. 85mL, 19.97±1.56mm, and 0.04±0.01, higher than those in the control group 24. 05±1. 64mL, 14. 07±1. 48mm, 0. 01±0. 003, respectively, and the difference was statistically significant (P<0. 05). ·CONCLUSION: The intraocular fatty acid content, ocular outgrowth and intracellular expression of AdPLA mRNA in TAO patients are all higher than those in normal subjects. The increase of AdPLA expression in the adipose tissue of eyes of TAO patients resulted in a decrease in the amount of fat hydrolysis and an increase in fat accumulation and increased emphasis.
ABSTRACT
AIM: To observe the effects of porous polyethylene ( Medpor) as a spacer graft in the reconstruction of large areas eyelid defect after the operation of malignant tumors of lower eyelids. METHODS: Nineteen cases ( 19 eyes ) of malignant tumors of lower eyelid underwent the eyelid reconstruction were selected. Medpor lower eyelid inserts implantation were used to replace tarsal joint sliding conjunctival flap and pedicle flap, and repaired full -thickness lower eyelid defects then underwent eyelid reconstruction. RESULTS: Appearance of eyelids and functional improvements were satisfactory with no stimulation on the eyeball and no effect on the visual function. Implants is with no absorption, shift, exclusion or infection and no tumor recurrence in all cases during the follow up for 6-36mo. CONCLUSION: Medpor lower eyelid inserts implantation can instead tarsal plate for the reconstruction of medium to large areas lower eyelid defect, which is easy performing with rare complications. It is an ideal alternatives of tarsal plate.