ABSTRACT
Objective To enhance the yield and product quality of diosgenin extract,the method of stepwise biocatalysis was used.Methods The optimum conditions of slepwise biocatalysis were obtained through orthogonal tests.In qualitative and quantitative analysis of diosgenin extracted through stepwise biocatalysis,IR spectrum and HPLC chromatogram were used.The yield and melting point of diosgenin were taken as investigated standard index to compare this method with others,such as acid hydrolysis,spontaneous fermentation,and enzymatic hydrolysis.Results When cellulase and pectinase compound,amlyse and diastatic enzyme were added in order in the method of stepwise biocatalysis,98% diosgenin from plants was extracted.IR spectrum of the product was in accordance with that of reference substance and the purity of it is more than 95% according to its HPLC chromatogram.Conclusion Compared with other three methods,it can improve the yield and quality with less energy consumption by stepwise biocatalysis.