ABSTRACT
Objective: To study the effects of physical and chemical factors on hairy root of Panax ginseng and its glycoside production. Methods: The cell growth index and its glycoside yield in different culture condition such as different plant hormones mixed, mediums and time were compared. Results: After hairy root of Panax ginseng was precultivated on the MS medium with IBA (0.5%) at first 72 hours. the culture was cultured on the MS medium. During 6 weeks, dry weight of hairy root increased by 98.74 times, and the content of glycoside synthesized by hairy root is 5.190% and Re+Rg1 is 0.3271%, respectively. Conclusion:We have achieved the best condition of hairy root of Panax ginseng.
ABSTRACT
AIM: To establish a HPLC for the determination of rubimaillin in Shenqiangujing Granules (Radix Codonopsis, Radix Rubiae, Radix Rehmanniae, Fructus Ligustri Lucidi, etc.) METHODS: HPLC column was C-18 (250 mm?4.6 mm, 5 ?m). The mobile phase was methanol-water-tetrahydrofuran (310∶90∶3). Flow rate was 1.0 mL?min -1 . The detection wavelength was at 249 nm. RESULTS: The linear range of rubinaillin was in the range of 0.056～0.29 ?g, r=0.9999. The average recovery was 99.36% (RSD=1.60%, n=5). CONCLUSION: The method is accurate with the good reproducibility, and can be used for the determination of rubimaillin in Shenqiangujing Granules.