ABSTRACT
Rapid advances in nanotechnology are setting new paradigms in science and technology. Nano-forensic offers a fresh perspective for real-time investigation of crimes with established advanced nano-imaging tools for visualization, nano-manipulators and nanosensors. Nanotechnology enables enhanced and improved efficiency of traditional and applied forensic techniques with high sensitivity, accuracy, and reduced requirement of time. Therobust nanotechnological applications of classic tools for investigating the documents in question, time since death, age of bloodstains, along with its application in DNA analysis, in improving polymerase chain reaction (PCR) efficiency, and explosive detection. In microbial forensics, only an extensive collection of strains from around the world and high-quality sequence data can provide the basis for meaningful results. Nanotechnology will help faster disclosure of cases and evidence to competent courts, will quickly identify proxies, interpret sources, and present those findings in court as evidence.Forensic palaeontology relies heavily on pollen fingerprinting which is one of the most effective methods for detecting and analyzing evidence of exposure. Nano-based strategies hold enormous future in detecting latent fingerprinting, for illicit drug screening and security features. Nanotechnology is likely to play an important role in forensic science, providing reliable evidence in addition to being more selective and sensitive. This review offers valuable insights into the applications of nanoparticles in forensic science.
ABSTRACT
Gladiolus spikes with fully turgid petals need to have a longer shelf life to fetch a higher price as well as display value. To improve the life duration of ornamental flowers, the ability of plants to produce silver nanoparticles (AgNP) was exploited. The ability of plants to produce AgNP when treated with silver nitrate solutions was juxtaposed by using Gladiolus (cut flowers): (i) To find the appropriate concentration of AgNO3 suitable for increasing shelf-life of Gladiolus cut flowers; (ii) To prepare silver nanoparticle from AgNO3; and (iii) To confirm the formation of silver nanoparticle using UV-vis spectrophotometry. Two different reductants (dehydrogenases present at the surface of the plant cells and sucrose) demonstrated the reduction of Ag+ to generate AgNPs. DLS (Dynamic Light Scattering) study revealed the presence of NPs in the AgNO3 salt solution incubated with Gladiolus cut flowers. The DLS data also suggested that the size of AgNPs decreased with increasing concentration of AgNO3. In the present study, along with silver nitrate, sucrose was also used. The shelf life and display value of the cut Gladiolus can be increased and optimized by incubating it in sucrose solution in combination with AgNO3.
ABSTRACT
We report the efficacy of the gold nanoparticles (AuNPs) synthesized using the leaf extracts of Syzygium cumini (common name Jamun) with auric chloride (AuCl4) which was used as both reducing and capping agent at room temperatures- 25°C. Synthesized AuNPs were characterized using UV-Vis spectroscopy indicating a peak in the range of 520-540 nM. The hydrodynamic radii measured by DLS clearly indicated the size of AuNPs in the range of 14-64 nM. The biological efficacy in terms of antimicrobial activity was assessed by the Kirby Bauer method, applied for both Gram-positive and Gram-negative bacteria such as Staphylococcus aureus and Escherichia coli, respectively. The Zone of inhibition (ZOI) diameter was found to be 4 mM and 3 mM in S. aureus and E. coli, as indicated by the bactericidal activity. Hence, AuNPs synthesized by green synthesis are proposed as economical, environment friendly with immense potential as an antibacterial agent and for drug delivery.