ABSTRACT
Context: the Tehran Lipid and Glucose Study [TLGS] is a community-based study to reveal the frequency of non-communicable diseases [NCDs] in Tehran's population. This research consists of two main parts, a cross-sectional study on the prevalence of cardiovascular risk factors and a 20-year-ongoing prospective cohort study, which was initiated in 1999 in several phases with an approximate duration of 3.6 years, and is still ongoing. The aim of the present study is review the 20 year biochemical findings of the TLGS related to the NCDs in a large sample
Methods: all articles on biochemical assessments derived from the TLGS from the earliest publications [2002] until 30 January 2018 were reviewed for their findings on different risk factors of NCDs
Results: according to the TLGS findings high sensitivity C-reactive protein [hs-CRP], interleukin-6 [IL-6], homocysteine [Hcy], age, smoking, hypertension, and obesity were the most important risk factors of cardiovascular diseases [CVD]. It was illustrated that in subjects with abdominal obesity, the hs-CRP and IL-6 serum levels were higher than in normal subjects. The most appropriate prognostic indexes and associations were for hs-CRP, IL-6, and Hcy with abdominal obesity, waist circumference, WHtR, and wrist circumference, respectively. Previous studies have demonstrated a direct relationship between obesity and serum levels of inflammatory factors
Conclusions: according to the results of TLGS, serum levels of biochemical risk factors such as hs-CRP, IL-6, and Hcy could be beneficial in early diagnosis and effective treatment of cardiovascular, obesity and other metabolic diseases
ABSTRACT
TPO gene variations are one of the causes of thyroid autoimmune diseases. The aim of this study was to examine the association between the T1936C, T2229C and A2257C SNPs [single nucleotide polymorphisms]of the TPO gene and Anti-TPO level. In this case-control study, 188 individuals [86 males and 102 females], aged 20-80 years, were randomly selected from the Tehran Lipid and Glucose Study population [TLGS]. A2257C and T2229C SNPs were detected with RFLP by use of BsrI and Eco57I as restrictive enzymes respectively, while the T1936C SNP was determined with ARMS-PCR. In the presence of the C allele of T1936C, Anti-TPO level was significantly increased [CC: 238 +/- 43.3, CT: 47.7 +/- 15.9, TT: 74.1 +/- 11.3 IU/L; p 0.002]; however, it disappeared after adjustment for sex and age [p 0.059]. No significant difference, before and after adjustment, was found in Anti-TPO level in the presence of T2229C SNP [CC: 129.1 +/- 24.5, CT: 43.5 +/- 12.6, TT: 126.5 +/- 13.8 IU/L; p 0.196]. The association of A2257C with Anti-TPO level was only significant after adjustment for sex and age [p 0.007],and between the ATC ,CTT haplotypes and Anti-TPO level ,the association was significant [p 0.023, 0.021]; however, the association it was dominant between CTT and anti-TPO concentration was significant only after adjustment for sex [p 0.014]. The result of this study, showed age and sex as potential confounders which could modify the association between TPO polymorphisms and Anti-TPO level in a Tehranian population
ABSTRACT
Obesity cause a lot of disease such as cardiovascular, type 2 diabetes, obstructive sleep apnea, some types of cancers and osteoarthritis. Reports show polymorphism at position -308 in the promoter region of TNF-alpha increase transcription of the gene in adipocytes. We, therefore, examined the relationship between this variant and BMI as an obesity criterion and other obesity affective factors in an Iranian population. 244 Subjects were randomly selected from the Tehran lipid and Glucose study which is a Cross Sectional Study and they were classified in two age groups under 18 and above 18. We measured FBS, HDL-C, LDL-C, triglyceride, cholesterol levels, CRP, IL-6, IL-10, Adiponectin, and HOMA for all individuals. Factors including Body mass index and blood pressure were measured too. A 107bp segment of the mentioned gene with PCR [Polymerase Chain Reaction] was amplified and the polymorphism with RFLP [Restriction Fragment Length Polymorphism] revealed. Undistributed Data were analyzed by kruskal-Wallis test and distributed Data were analyzed by ANOVA. We used one-way ANOVA test followed by post hoc multiple comparison [Tukey] to compare laboratory findings of BMI groups. All data were analyzed using SPSS 16. The allele frequency of TNF-alpha polymorphism was in the Hardy Weinberg equilibrium and the Genotype of all 244 subjects were GG[85.7%], GA[13.1%],AA[1.2%] and there was no relation between BMI and the frequency of this allele. In adolescent sbp] systolic blood pressure] in girls at GA state was significantly more than GG and CRP in boys at AA state was significantly more than the two other state In adult cholesterol in men at AA was significantly more than the two other state and LDL-C in women in GG was more than the two other state. Data from this study shows no association between G-308A TNF-alpha promoter polymorphism with increasing BMI, so probably it is not important risk factor for obesity in Iranian populations