ABSTRACT
Thirty-two Trypanosoma cruzi strains, isolated from chronic chagasic patients in the northwest of the state of Paraná (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior
Subject(s)
Humans , Animals , Behavior, Animal , DNA, Protozoan , Trypanosoma cruzi , Genotype , Glycoconjugates , Minisatellite Repeats , Polymerase Chain Reaction , Polymorphism, Genetic , Random Amplified Polymorphic DNA Technique , Trypanocidal Agents , Trypanosoma cruziABSTRACT
The effectiveness of seven disinfectant compounds used in dentistry for a rapid decontamination of 32 gutta-percha cones adhered with Sraphylococcus aureus, Enterococcus faecalis, Escherichia coli strains or Bacillus subtilis spores was compared. Cones were treated with 2(per cent) glutaraldehyde, 1(per cent) sodium hypochlorite, 70(per cent) ethyl alcohol, 1(per cent) and 0.3(per cent) iodine alcohol, 2(per cent) chlorhexidine, 6(per cent) hydrogen peroxide, and 10(per cent) polyvinylpyrrolidone-iodine, for 1, 5, 10, and 15 minutes. After treatment, each cone was transferred to thioglycollate broth and incubated at 37ºC for 7 days. The products were bactericidal after 1 to 5 minutes and, with exception of ethyl alcohol and iodine-alcohol, sporicidal after 1 to 15 minutes of exposure. Results suggest that chlorhexidine, sodium hypoclorite, polyvinylpyrrolidone-iodine, hydrogen peroxide, and glutaraldehyde were the most effective products in the decontamination of gutta-precha cones.