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Indian J Ophthalmol ; 2008 Jan-Feb; 56(1): 65-6
Article in English | IMSEAR | ID: sea-70478

ABSTRACT

New molecular biological technique of Polymerase Chain Reaction (PCR) based Restriction Fragment Length Polymorphism (RFLP) can identify the species from paraffin-embedded tissue section. We demonstrated Aspergillus fumigatus fungus by PCR-based RFLP technique from paraffin section of an eyeball of an eight-month-old child removed for endogenous endophthalmitis.


Subject(s)
Aspergillosis/diagnosis , Aspergillus fumigatus/classification , Endophthalmitis/diagnosis , Eye Infections, Fungal , Humans , Infant , Male , Paraffin Embedding , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Tissue Fixation
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