ABSTRACT
Objective To study the impacts of leukocytes elevation on the sperm quality and oxidative stress .Methods A total of 52 elevated leukocytes specimen and 50 normal leukocytes specimen were collected .Standard semen analysis was performed .Re-active oxygen species (ROS) production was measured using luminol as the probe by the Chemiluminescence method .Malonalde-hyde(MDA) content in semen was measured by the sulfo-barbitone acid method .Total superoxide dismutase(T-SOD) in semen was measured by the xanthine oxidase method .Results The semen parameters including total motility (P= 0 .008) ,progressive motility (P= 0 .005) and normal forms (P= 0 .003) ,the sperm motion parameters including mean curvilinear velocity (VCL)(P= 0 .023) , mean straight-line velocity (VSL)(P= 0 .010) ,mean average path (VAP)(P= 0 .011) ,swing(WOB)(P 0 .05) .Conclusion The leukocytes elevation could result in the decrease of spermatozoa quality ,which might be related with ROS increase .
ABSTRACT
Objective To investigate the effects of two biovars of ureaplasma urealyticum on leukocyte concentration and sperm motion parameters.Methods Sperm motion parameters were analyzed with computer-aided sperm analysis (CASA).Leukocytic number of semen was measured with benziding-perxidase staining.Culturing and real-time polymerase chain reaction (RT-PCR) was used to identify biovars of U.urealyticum in the semen.Results Sperm motion parameters including mean curvilinear velocity (VCL), mean straight-line velocity (VSL), mean average path (VAP) , and mean beat Cross frequency (BCF) showed significant difference between infertile and normal group (P ＜0.05).Biovar Ⅱ infected men displayed significant increase in leukocyte counts (P ＜ 0.001).When compared to uninfected group,the sperm motion parameters showed significant decreases of VCL, VSL, VAP, mean percentage lineality (LIN), and swing(WOB) in biovar Ⅱ (P ＜ 0.05).Conclusions Biovar Ⅱ has closer relationship with leukocyte elevation and sperm motion impairment.