ABSTRACT
Residual paralysis, recurarization is defined as a remnant effect of neuromuscular blocking after surgery that can cause postoperative complications. Clinical complications of recurarization include dyspnea, gastric content aspiration, and atelectasis. Therefore, complete recovery of muscle strength at the end of surgery is a significant factor for patient safety. We report a case of a 53-year-old woman who presented with residual paralysis after total thyroidectomy. To improve her condition, we injected sugammadex intravenously in the post-anesthetic care unit. After that, we observed her for 1 hour and her muscle strength gradually recovered. She did not have any symptoms on the next day and was discharged on the 5th post-operative day.
Subject(s)
Female , Humans , Middle Aged , Dyspnea , Muscle Strength , Neuromuscular Blockade , Neuromuscular Blocking Agents , Neuromuscular Monitoring , Paralysis , Patient Safety , Postoperative Complications , Pulmonary Atelectasis , ThyroidectomyABSTRACT
MicroRNA (miRNA) pathways have been implicated in stem cell regulation. This study investigated the molecular effects of propofol on adipocyte stem cells (ASCs) by analyzing RNA expression arrays. Human ASCs were isolated by use of a liposuction procedure. ASCs were treated with saline, 50 microM propofol, or 100 microM propofol in culture media for 3 hours. After the isolation of total RNA, the expression of 76 miRNAs was evaluated with peptide nucleic acid-miRNA array analysis through denaturation and hybridization processes. Treatment with 50 microM propofol resulted in significant down-regulation of expression of 18 miRNAs and upregulation of expression of 25 miRNAs; 100 microM propofol resulted in significant downregulation of expression of 14 miRNAs and upregulation of expression of 29 miRNAs. The lowest expression was seen for miR-204, which was 0.07-fold with 50 microM propofol and 0.18-fold with 100 microM propofol. The highest expression was seen for miR-208b, which was 11.23-fold with 50 microM propofol and 11.20-fold with 100 microM propofol. Expression patterns of miRNAs were not significantly different between 50 microM and 100 microM propofol treatment. The results of this study suggest that propofol is involved in altering the miRNA expression level in human ASCs. Additional research is necessary to establish the functional effect of miRNA alteration by propofol.
Subject(s)
Humans , Adipocytes , Adult Stem Cells , Culture Media , Down-Regulation , Lipectomy , MicroRNAs , Propofol , RNA , Stem Cells , Up-RegulationABSTRACT
MicroRNA (miRNA) pathways have been implicated in stem cell regulation. This study investigated the molecular effects of propofol on adipocyte stem cells (ASCs) by analyzing RNA expression arrays. Human ASCs were isolated by use of a liposuction procedure. ASCs were treated with saline, 50 microM propofol, or 100 microM propofol in culture media for 3 hours. After the isolation of total RNA, the expression of 76 miRNAs was evaluated with peptide nucleic acid-miRNA array analysis through denaturation and hybridization processes. Treatment with 50 microM propofol resulted in significant down-regulation of expression of 18 miRNAs and upregulation of expression of 25 miRNAs; 100 microM propofol resulted in significant downregulation of expression of 14 miRNAs and upregulation of expression of 29 miRNAs. The lowest expression was seen for miR-204, which was 0.07-fold with 50 microM propofol and 0.18-fold with 100 microM propofol. The highest expression was seen for miR-208b, which was 11.23-fold with 50 microM propofol and 11.20-fold with 100 microM propofol. Expression patterns of miRNAs were not significantly different between 50 microM and 100 microM propofol treatment. The results of this study suggest that propofol is involved in altering the miRNA expression level in human ASCs. Additional research is necessary to establish the functional effect of miRNA alteration by propofol.