Up-Regulation of MiR-1915 Inhibits Proliferation, Invasion, and Migration of Helicobacter pylori-Infected Gastric Cancer Cells via Targeting RAGE

PURPOSE: Helicobacter pylori (HP)-infected gastric cancer (GC) is known to be a fatal malignant tumor, but the molecular mechanisms underlying its proliferation, invasion, and migration remain far from being completely understood. Our aim in this study was to explore miR-1915 expression and its molecular mechanisms in regulating proliferation, invasion, and migration of HP-infected GC cells. MATERIALS AND METHODS: Quantitative real-time PCR and western blot analysis were performed to determine miR-1915 and receptor for advanced glycation end product (RAGE) expression in HP-infected GC tissues and gastritis tissues, as well as human gastric mucosal cell line GES-1 and human GC cell lines SGC-7901 and MKN45. CCK8 assay and transwell assay were performed to detect the proliferation, invasion, and migration capabilities. MiR-1915 mimics and miR-1915 inhibitor were transfected into GC cells to determine the target relationship between miR-1915 and RAGE. RESULTS: MiR-1915 was under-expressed, while RAGE was over-expressed in HP-infected GC tissues and GC cells. Over-expressed miR-1915 could attenuate cellular proliferation, invasion, and migration capacities. RAGE was confirmed to be the target gene of miR-1915 by bioinformatics analysis and luciferase reporter assay. Moreover, HP-infected GC cellular proliferation, invasion, and migration were inhibited after treatment with pcDNA-RAGE. CONCLUSION: MiR-1915 exerted tumor-suppressive effects on cellular proliferation, invasion, and migration of HP-infected GC cells via targeting RAGE, which provided an innovative target candidate for treatment of HP-infected GC.

Effect of fluoride ion on corrosion of two dental titanium alloys / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the effect of fluoride concentration on the corrosion behavior of Ti-12Zr alloy and Ti-6Al-4V alloy in a simulated oral environment.</p><p><b>METHODS</b>Each specimen was put into the acidic artificial saliva (pH = 4.0) with different NaF concentration (0%, 0.05%, 0.1%, 0.2%) and electrochemical experiment was carried out. Then the surface microstructure and compositions of specimen were analyzed by scanning electron microscope (SEM) after immersed in the test solution with constant temperature.</p><p><b>RESULTS</b>The corrosion potential (E(corr)) and polarization resistance (R(p)) values of titanium alloy decreased with the increasing of NaF concentration, while the corrosion current density (I(corr)) values increased with the increasing of NaF concentration in acidic artificial saliva. The E(corr), R(p) and I(corr) values of titanium alloy changed significantly when the NaF concentration was over 0.1% (P < 0.05). The R, between Ti-12Zr alloy and Ti-6Al-4V alloy had a statistically significant difference, and the R(p) of Ti-12Zr alloy was higher than that of Ti-6Al-4V alloy when the NaF concentration was 0.2% (P < 0.05).</p><p><b>CONCLUSION</b>The fluoride ions have a negative influence on the corrosion resistance of Ti-12Zr alloy and Ti-6Al-4V alloy, especially in the acidic artificial saliva which contained over 0.1% NaF.</p>