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Through the excellent experiment of cardiovascular system in pathology, the prac-tice of the teaching reform was carried out such as combining flow model with real specimens, digital sections with light microscope slides, inserting the use of special staining experiments in combination with digital medical image analysis and the simulation of cardiovascular clinical pathological case dis-cussions. Linking theory with experiment teaching and experiment teaching with clinical practice was focused on, which not only stimulated students' interest in learning and their exploring thinking and hands-on ability, but also promoted them to obtain good learning effect, thus improving the teaching quality.
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@#Objective To establish and evaluate a rat model of adriamycin-induced myocardial injury.Methods Tweenty-four male SD rats were randomly divided into two groups. The adriamycin (ADR) group (n=15) was injected intraperitoneally with six equal doses of ADR (2 mg/kg each time, with a cumulative dose of 12 mg/kg) over a period of two weeks, while the control group (n=9) received an equivalent volume of normal saline intraperitoneally alone. The Rats were observed for 5 weeks after treatment. The general conditions and mortality were recorded. At the end of experiment, the parameters of hemodynamics including LVSP, LVEDP, ±LVdP/dtmax and HR were measured. The pathological changes were analyzed by histological hematoxylin-eosin staining. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity as well as malondialdehyde (MDA) content in heart tissue were measured according to the kit instructions, respectively. Results The cumulative mortality in the ADR group was 40%. Compared with the control group, LVEDP and -LVdP/dtmax were significantly increased in the ADR group (P<0.001, P<0.05). The results of pathological examnation of the ADR group were consistent with characteristic changes of myocardial injury. Myocardial malondialdehyde (MDA) content was significantly increased and glutathione peroxidase (GSH-Px) activity decreased in the ADR group compared with those in the controls.Conclusion A rat model of adriamycin-induced myocardial injury has been successfully established by administration of ADR (cumulative dose, 12 mg/kg) to rats in six equal intraperitoneal injections over a period of 2 weeks, resulting in cardiac dysfunction and histological damages.
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AIM: To preliminarily study the molecular mechanism for the cardiac injury in rat by adriamycin and the mechanism for the acute repair in the body. METHODS: The male Sprague Dawley rats were randomly divided into four groups (n=10 in each): The first group was kept without treament and served as the control ;the second, the third and the fourth received ADR in different doses (10, 20, 40 mg?kg -1 , respectively) by injection of adriamycin. The content of malondialdehyde (MDA)in the serum was estimated with thiobarbituric acid. Cu Zn SOD was measured by its reaction with xanthine oxidase. GSH was measured by its reaction with 5, 5 nitrobenzoic acid. Using semi quantitative reverse transcription polymerase chain reaction (RT PCR),we analyzed the expression of the associated gene. RESULTS: MDA contents in the medium and high ADR dose groups were higher than that in the control group (P