ABSTRACT
Teachers are principal parts in the innovation and implementation of teaching.Along with the deepening transformation of new medical teaching mode,higher demands have been set for the construction of teachership and for the education and training of teachers.The article gives an analysis on several issues that should be given attention to in the teacher training.
ABSTRACT
The discussion method adequately applied to the teaching module of new medical teaching mode may promote the study of medical professional knowledge and cultivate the medical students' moral and cultural quality in the course of medical education,thus further optimizing the new medical teaching mode on a certain extent.
ABSTRACT
Objective: To construct eukaryotic expression vector of adenovirus type 5 fiber gene and investigate its expression in eukaryotic cell. Methods: By endonuclease digestion, fragment harboring the fiber gene was cloned into plasmid pBluescript M13- from the pAdEasy-1 to construct pBS/Fiber. The pBS/Fiber was then digested with XbaI and KpnI. The yielding fragment was subcloned into the pcDNA3.1, obtaining the eukaryotic expression vector pcDNA/Fiber. To test the eukaryotic expression ability of the pcDNA/Fiber, pcDNA/Fiber was transiently transduced into the COS-7 cells using lipofectamine, and the fiber protein was detected by SDS-PAGE and Western blot. Results: Both plasmid pcDNA3 1 and eukaryotic expression vector pcDNA/Fiber were successfully constructed. Anew protein band was detected by SDS-PAGE and Western blot, its molecular weight was 62 kD on denaturing SDS-PAGE gel and 186 kD on non-denaturing SDS-PAGE gel.Conclusions: The eukaryotic expression vector of the adenovirus type 5 fiber gene was expressed in the COS-7 cells. In natural state, the expressed fiber protein was a trimer. The plasmid pBS/Fiber can be used to construct target adenovirus vector.