ABSTRACT
Quinoa is a regionally important grain crop in the Andean region of South America. Recently quinoa has gained international attention for its high nutritional value and tolerances of extreme abiotic stresses. DNA markers and linkage maps are important tools for germplasm conservation and crop improvement programmes. Here we report the development of 216 new polymorphic SSR (simple sequence repeats) markers from libraries enriched for GA, CAA and AAT repeats, as well as 6 SSR markers developed from bacterial artificial chromosome-end sequences (BES-SSRs). Heterozygosity (H) values of the SSR markers ranges from 0.12 to 0.90, with an average value of 0.57. A linkage map was constructed for a newly developed recombinant inbred lines (RIL) population using these SSR markers. Additional markers, including amplified fragment length polymorphisms (AFLPs), two 11S seed storage protein loci, and the nucleolar organizing region (NOR), were also placed on the linkage map. The linkage map presented here is the first SSR-based map in quinoa and contains 275 markers, including 200 SSR. The map consists of 38 linkage groups (LGs) covering 913 cM. Segregation distortion was observed in the mapping population for several marker loci, indicating possible chromosomal regions associated with selection or gametophytic lethality. As this map is based primarily on simple and easily-transferable SSR markers, it will be particularly valuable for research in laboratories in Andean regions of South America.
Subject(s)
Amplified Fragment Length Polymorphism Analysis , Base Sequence , Chenopodium quinoa/genetics , Chromosome Mapping , DNA Primers/genetics , DNA, Plant/genetics , Genetic Markers , Minisatellite RepeatsABSTRACT
Se estudiaron 26 personas de sexo masculino que cumplían instrucción militar. A los 8 días de haber realizado maniobras en el agua, un soldado presentó hipertermia, mialgias y compromiso respiratorio, hepático y renal. A partir de este caso se realiza un estudio de campo en el resto del batallón que tenía iguales antecedentes epidemiológicos y se detectan en total 14 enfermos de leptospirosis. Esta enfermedad debe considerarse como riesgo en el personal de las Fuerzas Armadas por las actividades propias de las mismas