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Gac. méd. Méx ; Gac. méd. Méx;140(5): 507-512, sep.-oct. 2004. ilus, tab
Article in Spanish | LILACS | ID: lil-632176

ABSTRACT

Se llevó a cabo un estudio en la sierra sur de Oaxaca de aislamiento de cepas silvestres de Sporothrix schenckii a partir de muestras de tierra, así como investigación de reactores positivos a la intradermorreacción con Esporotricina. El estudio se llevó a cabo a partir de la recolección de muestras de tierra y su posterior procesamiento mediante métodos de dilución y aislamiento del hongo en medios habituales de cultivo de Sabouraud simple y Sabouraud con antibióticos (SS y SA). Las cepas presuntivas se sometieron apruebas de dimorfismo, formación de melanina y comprobación de virulencia en animales. Se investigaron los reactores positivos a la Esporotricina L (Levaduriforme). Se identificaron tres cepas presuntivas por sus características reproductivas, formación demelanina y capacidad virulenta. En la comunidad se investigaron a 144 individuos dando positivos a la Esporotricina L el 6.25%. Se comprueba el aislamiento de cepas virulentas de S. schenckii a partir de la naturaleza (suelo) y se comprueba primocontacto con éste en un porcentaje de la población estudiada.


We conducted a study in the southern mountains of the Mexican State of Oaxaca that consisted of isolation of wild Sporothrix schenckii strains obtained from soil samples and investigation of positive reactors to skin test reaction with sprotrichin antigen. The study was conducted by means of recollection of soil samples and processing of these with dilution methods and fungal isolation in ordinary culture media Sabouraud simple Agar with and without antibiotics (SS, SA). Suspected strains underwent dimorfism, melanin formation, and virulence confirmation tests. Investigation of positive reactors to sporotrichin Y (yeast) was also conducted. Three supposed strains were identified due to their reproductive characteristics, melaninproduction, and virulence. In the community; 144 individuals were studied, ofwhom6.25%werepositivetosporotichin. Isolation of virulent strains o/Sporotrhix schenkii from nature (soil) andprimoinfection of a percentage of the studied population were confirmed.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Glycosaminoglycans/immunology , Sporothrix/isolation & purification
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