Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia / 소아과

PURPOSE: Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia. METHODS: Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups: normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 mug/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control-stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI). RESULTS: In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 mug/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups. CONCLUSION: Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture.

Relationship between Atopic Status and Immunoregulatory Cytokines in Respiratory Syncytial Virus Bronchiolitis / 소아알레르기및호흡기학회지

PURPOSE: Recent studies have demonstrated and suggested that Interleukin (IL) -10 and IL-11 are implicated in the pathophysiology of RSV infection and may act in the regulation of inflammatory response. We measured IL-10 and IL-11 in nasal secretions of infants with acute RSV bronchiolitis to investigate if there is any difference in the production of these anti-inflammatory cytokines between atopic and non-atopic subjects. METHODS: We measured IL-10, IL-11 in nasal secretions of 44 infants (20 were atopic) with acute RSV bronchiolitis. The nasal secretion samples were obtained from patients on admission and stored immediately at -70degrees C until analysis. Atopy was defined as having at least one positive skin prick test to common allergens, a positive history of atopic dermatitis or age-matched, high serum IgE level. RESULTS: IL-10 and IL-11 increased significantly in nasal secretion of infants with acute RSV bronchiolitis. Both IL-10 and IL-11 were significantly lower in atopic patients than in non-atopic patients. There was no significant relation between the severity of symptoms and IL-10 or IL-11 levels. CONCLUSION: Our study showed that both IL-10 and IL-11 increased in nasal secretion during acute RSV bronchiolitis, and the levels were significantly lower in atopic patients than in non-atopic patients. It suggests that the airway inflammation induced by RSV may be different between atopic and non-atopic patients and this may be associated with lower induction of these anti-inflammatory cytokines in atopic patients.

Neuroprotective Effects of Minocycline in Rat Brain Cortical Cell Culture Induced by Hypoxia

PURPOSE: In vivo, minocycline appears to be neuroprotective. Thus, the neuroprotective effects of minocycline were studied in a rat brain cortical cell culture induced by hypoxia. METHODS: Cultured cells from the brains of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% CO2 and hypoxia groups treated with 1% CO2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with either 1 or 10 g/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a TUNEL assay control-stained with DAPI. RESULTS: Among the normoxia groups, the control and sample groups treated with 1 and 10 g/mL of minocycline were all statistically significantly different from each other. Meanwhile, among the hypoxia groups, although the control was significantly different from the sample groups, there was no statistically significant difference between the sample groups. When comparing the normoxia and hypoxia groups, there was a statistically significant difference between the control groups and sample groups treated with 1 g/mL of minocycline, yet no significant difference between the sample groups treated with 10 g/mL of minocycline. CONCLUSION: Minocycline was found to be neuroprotective in normoxia and hypoxia induced rat brain cortical cell cultures.