ABSTRACT
OBJECTIVE:To study the effect of Xiaoyao pill on animal uterus and to evaluate its efficacy in regulating menstruation to serve as theoretical basis for its clinical application.METHODS:The convulsion model of both ex vivo and in vivo uterus of rats was induced by oxytocin and the effects of Xiaoyao pill on the tension as well as the contraction frequency change of smooth muscle of uterus were observed.RESULTS:Xiaoyao pill could markedly inhibit the enhanced activities of either ex vivo or in vivo uterus of rats induced by oxytocin,inhibit the tension of smooth muscle and slow down the contraction frequency.CONCLUSION:Xiaoyao pill has satisfactory efficacy in regulating menstruation.
ABSTRACT
OBJECTIVE:To establish an HPLC method for determination of protocatechuic acid in Pterospermum heterophyllum.METHODS: The separation was performed on Agilent TC C18(250 mm?4.6 mm,5.0 ?m)column.Moblie phase consisted of acetonitrile-0.1% glacial acetic acid solution(pH 3.16,10 ∶ 90)with flow rate of 1.0 mL?min-1 and detection wavelength of 260 nm.The column temperature was set at 35℃ and injection volume was 10 ?L.RESULTS:The linear range of protocatechuic acid was 0.007 76～0.310 4 ?g(r=0.999 7) with an average recovery of 97.68% (RSD=1.51%,n=6).CONCLUSION:The method is simple,accurate and reproducible for the quality control of P.heterophyllum.