ABSTRACT
Actinomycetes are one of the most attractive sources of antibiotics. In the present studies, total of 15 strains were isolated from Dr. Ram Manohar Lohia Hospital and RML Park in Lucknow, U.P India. Isolated strains were identified for their antibacterial activity but only six isolate showed good result, they were evaluated for their inhibitory activity on 3 strains of microorganism (E. coli, P. aeruginosa and S. aureus). Isolation of Actinomycetes strain was obtained by serial dilution method and grown on actinomycetes isolation agar. Antibacterial compounds were produced by submerged fermentation and activity of compounds were checked against bacterial culture by antibiogram analysis where intracellular and extracellular compounds showed positive result, compare to intracellular compounds, extracellular compounds was showing best result which was 30 mm zone of inhibition against S. aureus and MIC was found to be 0.0009 mg/ml.