ABSTRACT
The clinical value of transvaginal ultrasound in clinical surgical treatment of cesarean scar pregnancy (CSP) was evaluated.The clinical data of 104 patients with CSP admitted at Tongji Hospital from 2013 to 2016 were collected and analyzed retrospectively,including the patients' age,gestational age,the size of gestational sac or uneven mass,the uterine scar thickness,β human chorionic gonadotropin (hCG) levels and so on.Of these 104 cases,30 cases were subjected to laparotomy,29 cases to laparoscopy,27 cases to hysteroscopy,16 cases to ultrasound-guided uterine curettage,and 2 cases to conservative treatment.The transvaginal ultrasound showed that uterine scar thickness and gestational sac or uneven mass size had significant difference (P＜0.05) among different surgical methods by comparatively analyzing the patients' data.It was suggested that transvaginal ultrasound may provide the valuable reference for choosing clinical surgical procedures for CSP.
ABSTRACT
During placental development,oxygen environment is not only critical for trophoblasts migration and invasion,but also fundamental for appropriate placental perfusion.Cysteine-rich 61 (Cyr61,CCN1) was expressed in the extravillous trophoblasts (EVTs) and decreased in preeclampsia.Its regulatory properties in human first-trimester extravillous trophoblast cell line (TEV-1 cells) upon a low oxygen tension were investigated.The present study examined functional changes involved in adaptation to hypoxia of the TEV-1 cells,using cobalt chloride (CoCl2) as hypoxic mimic.It was found that hypoxia inhibited growth of TEV-1 cells and induced the increase of cell apoptosis (P＜0.05).The Cyr61 expression in human EVTs was transcriptionally induced by CoCl2.Inappropriate EVTs apoptosis has been implicated in the failure of trophoblasts to fully invade and modify the uterine environment and Cyr61 down-regulation,potentially leading to preeclampsia.
ABSTRACT
The expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) was examined in the umbilical vessels of the patients with pre-eclampsia (PE) to explore its possible role in the pathogenesis of PE.The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR.The eNOS activity in umbilical vessels was spectrophotometrically detected.NO2-/NO3-,the stable metabolic end products of NO,was measured by using nitrate reductase.RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group (P<0.01).The activity of eNOS was significantly decreased in PE group [(12.83±3.61) U/mg] than in normal group [(21.72±3.83) U/mg] (P<0.01).The level of NO2-/NO3- in PE patients (27.53± 7.48) μmol/mg was significantly lower than that of normal group (54.27±9.53) μmol/mg (P<0.01).The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE (r=-0.58,P<0.01).It was concluded that the level of NOSTRIN expression was increased in umbilical vessel of women with PE,indicating that it may be involved in the pathogenesis of PE.