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<p><b>OBJECTIVE</b>To study the chemical constituents of Eriophyton wallichii.</p><p><b>METHOD</b>Compounds were separated and purified by column chromatographic methods, and their structures were elucidated by spectroscopic methods.</p><p><b>RESULT</b>Eight phenylpropanoids were isolated and identified as martynoside (1), leucosceptoside A (2), citrusin B (3), (+)-dehydrodiconiferyl alcohol-4, 9-beta-D-glucopyranoside (4), liriodendrin (5), velutinoside 11[ (6), jionoside B, (7), stachysoside D (8), respectively.</p><p><b>CONCLUSION</b>The eight compounds were firstly isolated from E. wallichii.</p>
Subject(s)
Arecaceae , Chemistry , Drugs, Chinese Herbal , Chemistry , Furans , Chemistry , Glucosides , Chemistry , Glycosides , Chemistry , Phenylpropionates , ChemistryABSTRACT
Objective: To investigate the inhibitory effect of Epibueropyridinium A on aldose reductase. Methods: Aldose reductase was extracted from cattle crystalline lens. Besides Epibueropyridinium A, the reactive system also contained DL-glyceraldehyde, aldose reductase, and NADPH. The activity changes of aldose reductase were detected at 340 nm. Epalrestat was taken as the positive control. The inhibitory type, Ki and IC50 were determined by double reciprocal plot, quadratic drawing, and drawing of inhibitor's concentration to inhibitory ratio, respectively. Results: Epibueropyridinium A significantly inhibited the activity of aldose reductase in a competitive manner, with IC50 being 4.2 μg/ml and Ki being 4.88 μg/ml. Conclusion: Epibueropyridinium A is competitive inhibitor of aldose reductase.
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Objective:To investigate the effect of Epibueropyridinium A (EA), extracted from Scrophularia ningpoensis, in prevention of D-galactose-induced cataract in rats. Methods: SD rats were randomly divided into 5 groups (n= 10), namely, the normal control, cataract modelq 20 mg/kg EA, 10 mg/kg EA, and positive control groups. The cataract model was induced by intraabdominal administration of D-galactose into rats. Rats of the 2 EA groups received corresponding amount of EA and those in the positive control group received epalrestat (10 mg/kg) or VitE (30 mg/kg). The changes of the lens were examined with slit lamp microscope at defined time points. The activities of superoxide dismutase(SOD) and the content of malonaldehyde (MDA) and sorbitol were determined in the lens 3 and 6 weeks later. Results: EA obviously improved the lens opacification in the 2 EA groups compared to cataract model group, and the improvement in high EA group was more obvious than that in the low EA group(P<0.01). The activities of SOD raised from (32.1±11.2) Nu · mg-1 in cataract model group to (65.9±16.7) Nu · mg-1 in low EA group and (83.2±15.6) Nu · mg-1 in high EA group(P<0.01). The content of MDA reduced from (26.72±5.36) nmol · mg-1 to (18.34±4.29) nmol · mg-1 in low EA group and (15.6±5.47) nmol· mg-1 in high EA group(P<0.01). The content of sorbitol in lens reduced from (24.33±2.57) μmol · g-1 to (15.46±2.07) μmol · g-1 in the low EA group and (11.45±1.39) μmol · g-1 in the high EA group after 3 weeks(P<0.01); and after 6 weeks, it reduced from (20.04±1.59) μmol · g-1 to (15.82± 1.42) μmol · g-1 and (13.22 ± 1.37) μmol · g-1, respectively (P<0.05 or 0.01). Conclusion: Epibueropyridinium A has preventive effect on development of sugar cataract in experimental models.
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Objective:To study the pharmacokinetics,thissue distribution and secretion of nerve growth factor(NGF)in mice.Methods:The conecntration of NGF in various body fluids and tissue were determined by isotope tracer combined SDSPAGE method.Results:The plasma concenmtration-time curve was in accordance with the two-compartment pharmacokinetic model.The elimation half-life(t1/2β)was 3.1.The half-life of distribution(t1/2ka)was 5min.Tpeak was 25 min.AUC was 72.4 mg·kg-1·h-1.The concentrations of NGF were high in thyroid,blood,submaxillary glands,superior cervical ganglion,adrenasl and kidneys.Conclusion:NGF has a wide distribution,high tissue concentrationa nd excrtet mainly through the urine.
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Objective:To study the pharmacokinetics,thissue distribution and secretion of nerve growth factor(NGF)in mice.Methods:The conecntration of NGF in various body fluids and tissue were determined by isotope tracer combined SDSPAGE method.Results:The plasma concenmtration-time curve was in accordance with the two-compartment pharmacokinetic model.The elimation half-life(t1/2β)was 3.1.The half-life of distribution(t1/2ka)was 5min.Tpeak was 25 min.AUC was 72.4 mg·kg-1·h-1.The concentrations of NGF were high in thyroid,blood,submaxillary glands,superior cervical ganglion,adrenasl and kidneys.Conclusion:NGF has a wide distribution,high tissue concentrationa nd excrtet mainly through the urine.