Differences of the structure, succession and function of Clostridial communities between jiupei and pit mud during Luzhou-flavour baijiu fermentation / 生物工程学报

Clostridia inhabiting in jiupei and pit mud plays key roles in the formation of flavour during the fermentation process of Luzhou-flavour baijiu. However, the differences of Clostridial communities between jiupei and pit mud remains unclear. Here, the species assembly, succession, and metabolic capacity of Clostridial communities between jiupei and pit mud were analysed by high-throughput sequencing and pure culture approaches. The ratio of Clostridial biomass to bacterial biomass in the pit mud was relatively stable (71.5%-91.2%) throughout the fermentation process. However, it varied widely in jiupei (0.9%-36.5%). The dominant Clostridial bacteria in jiupei were Clostridium (19.9%), Sedimentibacter (8.8%), and Hydrogenispora (7.2%), while Hydrogenispora (57.2%), Sedimentibacter (5.4%), and Caproiciproducens (4.9%) dominated in the Clostridial communities in pit mud. The structures of Clostridial community in pit mud and jiupei were significantly different (P=0.001) throughout fermentation. Isolated Clostridial strains showed different metabolic capacities of volatile fatty acids in pure culture. Spatial and temporal heterogeneity of Clostridial communities existed in the baijiu fermentation pit, which was closely related to the main flavour components of Luzhou-flavour baijiu.

Distribution of archaeal community in the mud pit during strong-flavor baijiu fermentation / 生物工程学报

Multi-species solid-state fermentation in a mud pit is one of the typical features of strong-flavor baijiu, in which archaea plays important roles, however, the archaeal community distribution and diversity during fermentation are still lack of research. The biomass, composition and succession of archaea communities in fermented grains and pit mud were analyzed by high throughput sequencing. The potential interaction between archaea and bacteria was analyzed by co-occurrence network. Results demonstrate that the average biomass of archaea in pit mud was about 200 times higher than that of fermented grains. There was no significant difference in archaeal community structure between fermented grains and pit mud (r=0.017, P=0.074), but succession patterns between them showed significant correlation (r=0.30, P=0.03). Methanobacterium was the most abundant archaea in fermented grains and pit mud, and other dominant groups included Methanosarcina, Methanocorpusculum, Methanoculleus, and Methanobrevibacter. The co-occurrence network analysis showed that Methanobacterium was positively correlated with most bacteria in fermented grains and pit mud, especially with Hydrogenispora and Caproiciproducens, the dominant bacteria in pit mud. Our results revealed the temporal and spatial distribution characteristics and potential functions of the archaeal community in the mud pit of strong-flavor baijiu.

Analysis of the Utilization of Gynecological Drugs for External Use in Our Hospital During the Period 2005～2007 / 中国药房

OBJECTIVE:To evaluate the utilization of gynecological drugs for external use in our hospital.METHODS:By a retrospective method,the gynecological drugs for external use used in our hospital from 2005 to 2007 were analyzed statistically in respect of the consumption sum,DDDs,DDC,etc.RESULTS:The consumption amount of the gynecological drugs for external use in our hospital remained stable over the 3 years,leading the first three places in terms of consumption sum were suppository,vaginal tablets and lotion,of which,the consumption sum of Chinese formulations and that of the western formulations were about the same.CONCLUSION:The use of the gynecological drugs for external use in our hospital over the 3 years is rational on the whole but remains to be improved further in order to contain the increasingly increased medical treatment cost.

Utilization Review of Chinese Patent Drugs for Gynaecological Patients in Our Hospital from 2004 to 2006 / 中国药房

OBJECTIVE: To investigate the utilization of Chinese patent drugs gynaecological patients in our hospital from 2004 to 2006 so as to promote rational drug use.METHOD:The Chinese patent drugs gynaecological patients used in our hospital from 2004 to 2006 were subjected to classification statistic and comprehensive analysis in respect of the consumption sum,DDDs and daily average cost.RESULT: Over the 3 years,the consumption sum of most of the Chinese patent drugs increased year on year,and the proportion of the cost of the Chinese patent drugs in the total increased as well.CONCLUSIONS:The use of Chinese patent drugs gynaecological patients in our hospital is basically rational. However,there are still some points remain to be improved.

Switch Therapy with Cefotaxime Sodium and Cefetamet in Female Patients with Urinary Tract Infection / 中国药房

0.05),however,the average cost in combined group was significantly lower than in single group(P