ABSTRACT
Objective To investigate potential biomarkers for different types of hand, foot and mouth disease(HFMD). Methods Nuclear magnetic resonance( NMR)-based metabolomics methods were used to analyze the metabolic differences in serum of HFMD and healthy controls. Partial least squares dis-criminant analysis(PLS-DA)and orthogonal partial least square discriminant analysis(OPLS-DA) were used for analysis of metabolite profiles. Differential statistical analysis was performed by t tests using GraphPad Prism 5 software. Results The levels of lipid,leucine,valine,β-hydroxybutyric acid,acetone,glucose,glyco-protein,glycerol,glycine,choline,trimethylamine oxide and lactate in HFMD increased significantly compared with healthy controls,whereas the levels of acetic acid and creatine decreased significantly. Additionally,the level of acetone further increased in the severe type of HFMD compared with the mild type,whereas the lev-els of lipid and trimethylamine oxide decreased. Conclusion These statistically significant differences are in-volved in lipid metabolism,glucose metabolism and protein metabolism in different types of HFMD. Lipid, acetone and trimethylamine oxide can be used as potential biomarkers of HFMD.
ABSTRACT
Objective@#To investigate potential biomarkers for different types of hand, foot and mouth disease(HFMD).@*Methods@#Nuclear magnetic resonance(NMR)-based metabolomics methods were used to analyze the metabolic differences in serum of HFMD and healthy controls.Partial least squares discriminant analysis(PLS-DA)and orthogonal partial least square discriminant analysis(OPLS-DA)were used for analysis of metabolite profiles.Differential statistical analysis was performed by t tests using GraphPad Prism 5 software.@*Results@#The levels of lipid, leucine, valine, β-hydroxybutyric acid, acetone, glucose, glycoprotein, glycerol, glycine, choline, trimethylamine oxide and lactate in HFMD increased significantly compared with healthy controls, whereas the levels of acetic acid and creatine decreased significantly.Additionally, the level of acetone further increased in the severe type of HFMD compared with the mild type, whereas the levels of lipid and trimethylamine oxide decreased.@*Conclusion@#These statistically significant differences are involved in lipid metabolism, glucose metabolism and protein metabolism in different types of HFMD.Lipid, acetone and trimethylamine oxide can be used as potential biomarkers of HFMD.
ABSTRACT
Objective To establishe the reference range of low hemoglobin density (LHD ％) of health population in Humen District of Guangdong,and discuss the clinical application value.Methods Randomly selected from January 2015 to September 2016 in Humen Hospital of Dongguan City physical examination of 1 650 cases of healthy people as control group,and selected the same period by the clinical diagnosis of iron deficiency anemia (iron-deficiency anemia,IDA) group,67 cases of patients with IDA Sysmex XT-1800i fully automatic blood analyzer were used respectively to detect blood routine,and then based on the average haemoglobin concentration (mean corpuscular hemoglobin concentration,MCHC),calculate the LHD％,and the calculation results were analyzed.Results The control healthy crowd LHD％ value was 2.41％ ± 0.85 ％,95％ reference range was 0.74 ％ ～4.08％,male was higher than the female,the difference was statistically significant (t =3.209,P＜0.05).IDA patients before treatment LHD％ was 30.97％ ± 18.65％,significantly higher than the control group,the difference was statistically significant (t=19.536,P＜ 0.01),and with the level of hemoglobin (Hb) showed a negative correlation (r=-0.4313).LHD％ values after treatment in patients with IDA group were significantly lower,but the recovery speed was slower than the Hb and MCHC.By ROC curve analysis,when the cut-off of LHD％ value was 4.10％,the LHD％ for IDA early diagnostic sensitivity of 96.78％,specificity of 100％.Conclusion Established the reference range of low hemoglobin density (LHD ％) of healthy population in Humen district of Guangdong,and LHD％ is IDA good indicator of early diagnosis and curative effect of dynamic monitoring.