ABSTRACT
AIM: To set up the method for adsorbing and separating total flavone in Radix Puerariae and in Folium Crataegi. By macroreticular resin. METHODS: UV Spectrophotometry was used to analyze the content of total flavone in mixture. RESULTS: The D101 was the best for adsorbing and separating total flavone in the following technological conditions: the maximum adsorbing capacity for flavone in Radix Puerariae was 10.10mg?g -1; the maximum adsorbing capacity for flavone in Folium crataegi was 11.28mg?g -1; the current velocity was 3ml?min -1, the eluting reagent 80% ethanol (20 times the volume of medicinal herbs) and the eluting velocity 3ml?min -1. CONCLUSION: D101 resin could be used to refine the total flavone in Radix Puerariae and in the Folium Crataegi.
ABSTRACT
Objective: To determine the content of sanguinarine and chelerythrine in Macleaya microcarpa(Maxim) Fedde. Methods: RP-HPLC was adopted, kromasil C 18 column (150mm?4.6mm.5?m) with a mobile phase acetonitrile-0.1%(V/V) phosphoric acid solution(25∶75) by UV detector at 270nm. Results: The average recoveries of chelerythrine and sanguinarine were 90.2%,92.8%,RSD were 1.5%,2.4% respectively. Conclusion: A simple, rapid and sensitive method with good precision was established.