ABSTRACT
Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine,L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry (ESI-MS) was achieved.Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions.The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation (CID) by MS/MS,which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer.Results Chiral recognition effect was achieved with the recognition rate (R) 1.61,2.92 and 2.17 for D-Lysine,L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds ofchiral amino acids were used as probes to distinguish between stereoisomers,and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.
ABSTRACT
Objective To evaluate the clinical relevance of maternal serum HCY,folic acid (FA)and vitamin B12 (VB12 )levels with pregnancy induced hypertension (PIH).Methods 100 pregnant women with PIH were selected as the observation group.100 non -PIH pregnant women at the same period in our hospital were selected as control group.Serum HCY,FA and VB12 levels at trimester,second trimester and third trimester of pregnancy were detected and compared.Results HCY levels at trimester,second trimester and third trimester of pregnancy were (15.07 ±4.86)μmol/L,(17.82 ±3.16)μmol/L and (19.25 ±3.24)μmol/L in the observation group,those were (6.36 ±2.47)μmol/L,(5.89 ±2.14)μmol/L and (6.68 ±2.08)μmol/L in the control group,prenatal HCY levels of the observation group in the three stages were significantly higher (t =15.97,31.26,15.97,all P 0.05).FA levels in the observation group at second trimester and third trimester were significantly lower than the control group (t =8.95,11.06,all P <0.05).VB12 levels at three stages of prenatal in the observation group were significantly lower than the control group (t =4.23,2.53, 4.23,all P <0.05).Conclusion Serum FA and VB12 levels are deficiency at second trimester and third trimester in PIH pregnant women,and with HCY rising,which may associated with the occurance and development of PIH.
ABSTRACT
The study aims to establish a quantitative nuclear magnetic resonance (QNMR) method for the determination of the absolute content of bosentan. Proton nuclear magnetic resonance spectroscopy [1H NMR] spectra were obtained in CDCl3 with the internal standard dimethyl terephthalate and zg30 pulse sequence by using a Bruker AVANCE II 400 spectrometer. The content of bosentan is determined with QNMR in comparison with the result obtained by mass balance method. The result is 96.25% by QNMR and 96.54% by mass balance method. A rapid and accurate QNMR method has been established for the quantitative determination of the absolute content of bosentan. The study provides a new way for the quality control and calibration of a new reference standard material, it could be the complementary with the mass balance method for the assay of standard reference.