ABSTRACT
To monitor the evolution of anastomoses performed on the distal colon in a situation of experimental peritonitis, 37 Wistar-Tecpar male rats a ged 114 to 130 days and weighing on average 298 g were divided into two lots: group S (control, N = 18) and group P (experimental, N = 19). P rats were submitted to laparotomy and peritonitis was induced by perforating the cecum with a needle, and S rats were only submitted to manipulation of the cecum. Twenty-four hours later the animals were resubmitted to laparotomy and distal colectomy was performed 1.5 cm distal to the peritoneal reflection. End-to-end anastomosis was performed on a single extramucosal plane using 8 separate stitches with 5-0 polypropylene sutures. The anastomoses were checked on the 3rd and 7th postoperative day. Upon opening the peritoneal cavity, the presence of alterations such as peritonitis or abscesses, adhesions, organs involved, fistulas or dehiscences was recorded. A 4.0 cm segment of the colon containing the anastomosis was resected and rupture pressure was measured. Epithelialization of the mucosal wound was evaluated and the material was studied histopathologically for inflammatory reaction and scar condition. Upon relaparotomy, peritonitis was detected in all P animals and fibrin was observed in the cavity of all animals. Adhesions were present in 2 groups, without significant differences between them. Mean rupture pressure was 108.7 mm Hg in group S and 112.0 mm Hg in group P on the 3rd day and 205.0 mm Hg in group S and 206.6 mm Hg in group P on the 7th day, with no significant difference between groups. Microscopic evolution was similar in the two groups. These results permit us to conclude that peritonitis induced by this method does not modify the healing process of distal colon anastomoses in rats.
Subject(s)
Animals , Male , Rats , Anastomosis, Surgical , Wound Healing , Colon/surgery , Peritonitis/chemically induced , Peritonitis/surgery , Evaluation Study , Surgical Wound Infection , Peritonitis/pathology , Photomicrography , Postoperative Period , Rats, Wistar , Time FactorsABSTRACT
A metoclopramida é considerada um antagonista da dopamina. Uma vez que a dopamina inibe a liberaçäo de prolactina pela hipófise anterior é de se esperar que o uso da metoclopramida leve a um aumento dos níveis séricos de prolactina. O seu uso por longos períodos levaria ao aumento do volume das mamas, galactorréia e distúrbios menstruais. Procurando investigar os efeitos desta substânvia sobre os órgäos genitais femininos, utilizaram-se 30 ratos, fêmeas virgens, Wistar-Tecpar, com idade entre 83 e 90 dias e peso variando de 160 a 249 gramas, divididos em dois grupos de 15 animais cada. O grupo experimento recebeu metoclopramida na dose de 10mg/dia por via intraperitoneal e o grupo controle, soluçäo fisiológica de mesmo volume. As aferiçöes foram realizadas no oitavo, décimo sexto e vigésimo quarto dias, quando eram analisados: mamas, ovários e útero. Pode-se constatar que as glândulas mamárias apresentavam-se bem desenvolvidas nos animais com 16 e 24 dias de medicaçäo (p16 = 0,001 e p24 = 0,0003), mostrando maior concentraçäo de alvéolos e ductos. Estes continham secreçäo no seu interior, porém näo houve diferença significante entre os dois grupos. No ovário observou-se a predominância de folículos secundários e o útero mostrou endométrio com características proliferativa em todos os períodos estudados em ambos os grupos. Estes dados sugerem que a metoclopramida atua sobre a glândula mamária estimulando seu desenvolvimento, porém näo se pode constatar influência significante sobre o ovário e útero com esta dose, via de administraçäo e tempo de administraçäo, em ratas virgens