ABSTRACT
Simian rotavirus SA-11, experimentally seede, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8000-fold concentration. By this method, a mean recovery of 81 per centñ7.5 of the SA-11 virus was achieved.
Subject(s)
Rotavirus , Wastewater/analysis , Immunoenzyme TechniquesABSTRACT
The present study investigates, by means of a combined enzyme immunoassay for rotaviruses and adenoviruses (EIARA), the occurrence of rotaviruses in stools and jejunal juices from 31 children with acute diarrhea and 18 with chronic diarrhea. Stools from 8 acute cases contained rotaviruses (26%). In two of these cases rotaviruses were also detected in the jejunal juice. In the chronic diarrhea group we identified rotaviruses in the stools of one patient and in the jejunal juice of another. Some of the electropherotypes of the rotaviruses identified showed different patterns of RNA migration. Abnormalities of the jejunal mucosa were characterized in 6 acute rotavirus-positive cases. No morphological or functional abnormalities of the intestinal mucosa were observed in the chornic diarrhea rotavirus-positive cases
Subject(s)
Infant , Humans , Male , Female , Diarrhea, Infantile/etiology , Rotavirus/pathogenicity , Electrophoresis, Polyacrylamide Gel , Feces/microbiology , Genes, Viral , Immunoenzyme Techniques , Jejunum/metabolism , Rotavirus/genetics , Rotavirus/isolation & purification , Intestinal Secretions/microbiologyABSTRACT
Definem-se dois soros padroes brasileiros para a conversao dos titulos inibidores da hemaglutinacao para a rubeola em Unidades Internacionais, usando um soro padrao ingles, com vistas a uniformizacao dos resultados e sua comparabilidade, entre diferentes laboratorios