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OBJECTIVE:To investigate the effects of Xiaosuanzao decoction on spontaneous motion and sleep time in mice, and to study its disassembled formulation. METHODS:Seven ingredients of Xiaosuanzao decoction were selected as the influential factors, including Ziziphus jujuba, Poria cocos, Ligusticum chuanxiong, Anemarrhena asphodeloides, Glycyrrhiza uralensis, Zingiber officinale and Cortex cinnamoni. Two levels of with or without were designed for each factor,and the experiment was ar-ranged according to L8(27) orthogonal test table. The effects of Xiaosuanzao decoction and each ingredient on spontaneous motion within 2 min and sleep time induced by superthreshold dosage of pentobarbital sodium were compared by visual analysis method and analysis of variance. RESULTS:Z. jujube and A. asphodeloides were the main active ingredients of Xiaosuanzao decoction on spontaneous motion;Z. jujube was the main active ingredient of Xiaosuanzao decoction on sleep time induced by superthreshold dosage of pentobarbital sodium. CONCLUSIONS:Xiaosuanzao decoction can inhibit spontaneous motion and prolong sleep time in-duced by superthreshold dosage of pentobarbital sodium,and Z. jujube is the main ingredient of Xiaosuanzao decoction.
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OBJECTIVE:To establish the method for the content determination of 4 active components in Compound xiaosuanzao chewable tablets.METHODS:HPLC-ELSD method was adopted.The determination was performed on a Grace Brava C18-BDS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The column temperature was 25 ℃,and sample size was 20 μL.The drift tube temperature is 100 ℃,and the carrier gas flow rate is 2.9 L/min.RESULTS:The linear ranges of betulic acid,betulinol,pachymic acid and glycyrrhizic acid were 44.50-890.0 μg/mL (r=0.999 3),20.28-405.6 μg/mL (r=0.999 7),20.50-656.0 μg/mL(r=0.999 7) and 10.50-336.0 μg/mL(r=0.999 6),respectively.RSDs of precision,stability and reproducibility were all lower than 3.0％.The recoveries were 99.44％-101.12％ (RSD=0.57％,n=6),99.41％-100.39％ (RSD=0.34％,n=6),99.31％-100.46％ (RSD=0.51％,n=6),98.96％-101.19％ (RSD=0.84％,n=6),respectively.CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 4 active components in Compound xiaosuanzao chewable tablets.
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Aim To develop a sensitive, specific and accurate method for the pharmacokinetic study of QO-58 ( a novel M channel opener ) in rats after intragas-tric ( ig) and intravenous ( iv) administration. Meth-ods QO-58 was administered at the doses of 25,50, 100 mg · kg-1 ( ig ) and at single dose of 100 mg · kg-1(iv), respectively. Blood samples were obtained at intervals after each administration. Plasma samples were deproteinized with acetonitrile after addition of in-ternal standard, and detected by RP-HPLC. The main parameters of pharmacokinetics were calculated by DAS2. 1. 1 software. Results The calibration curve in plasma was linear over the range of 0. 1 ~160 mg · L-1 in rat plasma, and the limit of detection ( LOD) was 0. 1 mg · L-1 . The intra-day and inter-day RSD was less than 20%. The recovery of QO-58 in rat plas-ma was 89. 56% ~101. 38%. The concentration-time curves of QO-58 in rat palsma were consistent with the two-compartment model after both oral and intravenous administration. The main pharmacokinetic parameters for QO-58 following oral administration with three doses (25, 50, 100 mg· kg-1 ) in rat were as follows:Cmax (mg·L-1):8.25,16.29,18.27;T12β(h): 8.24, 5. 01, 5. 92; AUC0-∞ ( g · min · L-1 ):261. 94, 189. 57,90. 65. Conclusion The developed method is simple and specific, and is suitable for preclinical pharmacokinetic studies of QO-58 .
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AIM:A HPLC linear gradient elution method was studied for determining notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 content in Xinnao guantong Guttate Pill.METHODS:The YMC-Pack ODS-A column was used with a mobile phase of acetonitrile-water 0-15 min(23∶77),16-40 min(50∶50)gradient elution,the wavelength of detecter was set at 203 nm.RESULTS:The linear ranges of R_1,Rg_1,Rb_1 were 0.204-1.836,0.818-7.362,0.858-7.722 ?g,respectively.The average recoveries of R_1,Rg_1,Rb_1 were 97.86%,101.71%,101.44%,respectively.CONCLUSION:The method is rapid,accurate,sensitive and reliable.The results show that method can be used to control quality of products.