ABSTRACT
SARS-CoV-2 has caused tens of thousands of infections and more than one thousand deaths. There are currently no registered therapies for treating coronavirus infections. Because of time consuming process of new drug development, drug repositioning may be the only solution to the epidemic of sudden infectious diseases. We systematically analyzed all the proteins encoded by SARS-CoV-2 genes, compared them with proteins from other coronaviruses, predicted their structures, and built 19 structures that could be done by homology modeling. By performing target-based virtual ligand screening, a total of 21 targets (including two human targets) were screened against compound libraries including ZINC drug database and our own database of natural products. Structure and screening results of important targets such as 3-chymotrypsin-like protease (3CLpro), Spike, RNA-dependent RNA polymerase (RdRp), and papain like protease (PLpro) were discussed in detail. In addition, a database of 78 commonly used anti-viral drugs including those currently on the market and undergoing clinical trials for SARS-CoV-2 was constructed. Possible targets of these compounds and potential drugs acting on a certain target were predicted. This study will provide new lead compounds and targets for further and studies of SARS-CoV-2, new insights for those drugs currently ongoing clinical studies, and also possible new strategies for drug repositioning to treat SARS-CoV-2 infections.
ABSTRACT
Objective To explore the effect of Wuji Solution (He Ji Ju Fang) on excreting IL-8 of SGC-7901 cells infected with Helicobacter pylori (H?pylori) in vitro. Methods H?pylori was inoculated to SGC-7901 cells. ELISA was applied to examine the production of interleukin-8 (IL-8) of cells infected with H?pylori. Results The production of IL-8 of infected cells was lower than that of control group (P
ABSTRACT
Objective To investigate the protective effect of Qiweibaizhu Powder against HRV infection of intestinal mucosal epithelial cells of suckling mice.Methods The 5-day-old NIH mice were made HRV diarrhea model by oral infection,and randomly divided into six groups:normal group(NG),model group(MG),Qiweibaizhu Powder group(BG),ribavirin group(ZG),suckling mice in each group were instilled corresponding drugs 3 times/d(NG and MG with normal saline) through the mouth.5 d after treatment,suckling mice were killed,small intestine stool was taken to test HRV,and pathological changes in small intestinal mucosa were observed by HE staining.Results Intestinal faeces HRV clearance of BG was significantly better than MG(P
ABSTRACT
Objective To explore the effect of Dingchuantang on the expression of NGF in the lung and thymus of asthmatic rats. Methods Thirty two Wistar rats were randomly divided into control group, asthmatic group, Dingchuantang group and Beclomethasone group, 8 rats for each group. Asthmatic model was made except the control group. The change of NGF expression in the lung and thymus were observed by means of immunohistochemistry. Results The expression of NGF in the lung and thymus of asthmatic group was much higher than that of control group and that of Dingchuantang group was much lower than that of asthmatic group. Conclusion Over-expression of NGF might be involved in the pathogenesis of bronchial asthma, and inhibition of NGF expression by Dingchuantang may be one of the mechanisms in the treatment of asthma.
ABSTRACT
Wuhutang, a well known traditional Chinese. herbal decoction for the treatment of as thma was found to havc an antiviral effect on respiratory syncytial virus (RSV ) both invitro and in vivo. A 50% reduction of plaque. formation was observed op Hep-2 cell monolayer at a concentration of 1:80. At such concentration, the. value of lg TC ID50 of. Viralyield was 2, 1, and 55% RNA synthesis of RSV was inhibited. It was found that among thefive medicinal herbs in prescription Ephedra sinica Stapf is responsible for the antiviralactivity on RSV. IC50 for plaque inhibition was found to be 1.6mg/ml. In animal model,Wuhutang singn ficantly reduced the titre of RSV and accelerated RSV clearance in mice,and alleviated viral pneumonitis by either oral or aerosol route.