ABSTRACT
El virus herpes simple tipo 2 (VHS-2), es una infección de transmisión sexual prevalente en el mundo a pesar de los avances diagnósticos y terapéuticos. Según estimaciones en el 2012, se calculaba que existían 417 millones de personas infectadas en todo el mundo. El objetivo de este proyecto fue detectar el VHS-2 en mujeres indígenas del estado Zulia, para ello se seleccionaron 100 mujeres de las etnias wayúu y añú sin discriminar edad y/o paridad. El médico procedió a realizar la toma de muestras con un hisopo de alginato, para el procesamiento de las mismas se utilizó el método de Reacción en Cadena de la Polimerasa (PCR) para detectar el VHS-2, en muestras de origen cérvico-uterino, y el diagnostico citológico se realizó a través de la coloración con hematoxilina- eosina. Se obtuvo un 22% de muestras positivas para VHS-2, los hallazgos obtenidos, demuestran que un porcentaje considerable de las pacientes presentan infección por el VHS- 2, siendo este de suma importancia debido a que el mismo puede contribuir a la progresión de procesos inflamatorios persistentes.
The herpes simplex virus type 2 (HSV-2) is a sexually transmitted infection prevalent in the world; in spite of despite diagnostic and therapeutic advances. According to estimates in 2012, it was calculated that there were 417 million people infected worldwide. The objective of this project was to detect HSV-2 in indigenous women of the Zulia state, for that 100 women of the Wayúu and Añú ethnic groups, were selected without discriminating age and/or parity. The physician proceeded to sample with an alginate swab, for the processing of the same was used the polymerase chain reaction (PCR) method to detect HSV-2 in samples of cervical-uterine origin, and cytological diagnosis was made through staining with hematoxylin-eosin. 22% of HSV-2 positive samples were obtained, the results show that a considerable percentage of patients present HSV-2 infection, which is extremely important because it can contribute to the progression of processes inflammatory agents.
ABSTRACT
Para determinar la prevalencia de parásitos intestinales, rotavirus y adenovirus en niños aparentemente sanos, se realizó el estudio parasitológico de 101 muestras fecales de individuos de uno u otro sexo, con edades comprendidas entre 2 meses y 14 años, así como el estudio virológico en 70 muestras de los niños menores de 5 años. A cada espécimen fecal se le realizó un examen coproparasitológico directo, concentración de Formol-Éter y coloración de Kinyoun. Para determinar Rotavirus y Adenovirus se empleó la técnica de Ensayo Inmunoenzimático (ELISA). Para el análisis estadístico se utilizó el Ji-cuadrado (χ²) y la prueba Z. Se observó un elevado porcentaje de individuos parasitados (85,15 por ciento), los protozoarios más frecuentes fueron: Blastocystis hominis (52,47 por ciento), Giardia lamblia (26,73 por ciento) y Entamoeba coli (20,79 por ciento) y entre los helmintos Trichuris trichiura (50,49 por ciento), Ascaris lumbricoides (48,51 por ciento) y Strongyloides stercoralis (7,92 por ciento). Se observó una frecuencia de Rotavirus del 2,86 por ciento y ausencia de infección por Adenovirus. La elevada prevalencia parasitaria demuestra las condiciones de insalubridad de la comunidad, a pesar de ello, lo contrario ocurrió con los virus estudiados. Para Rotavirus quizás influyó la adquisición de anticuerpos a través de la lactancia materna, mientras que para Adenovirus entéricos, probablemente el desarrollo de anticuerpos a edad temprana
To determine the prevalence of intestinal parasites, rotavirus and adenovirus in apparently healthy children, a parasitological study of 101 fecal samples from persons between 2 months and 14 years from both sexes was performed, as well as a virological study of 70 samples of less than 5 years of age. For each fecal specimen, a direct coproparasitological examination was performed, with Formol - Ether concentration and Modified Zielh-Nielsen tincture techniques was made. To determine the Rotavirus and Adenovirus, the immunoenzymatic assay technique (ELISA) was used. The Ji-square (c2) and the Z test were used for statistical analysis. A high percentage of people with parasites was found (85.15 percent). The most frequent protozoan species were: Blastocystis hominis (52.47 percent), Giardia lamblia (26.73. percent) and Entamoeba coli (20.79 percent); among the helminthes were Trichuris trichiura (50.49 percent), Ascaris lumbricoides (48.51 percent) and Strongyloides stercoralis (7.92 percent). Rotavirus frequency was 2.86 percent, while Adenovirus infection was absent. The high prevalence of parasites demonstrates unhealthy conditions in the community; despite these, the opposite occurred regarding the virus being studied. Perhaps the acquisition of antibodies through maternal lactation influenced the Rotovirus percentages, whereas development of antibodies at an early age probably influenced the enteric Adenovirus
Subject(s)
Humans , Male , Adolescent , Female , Infant , Child, Preschool , Child , Adenoviridae Infections/parasitology , Enterovirus Infections/parasitology , Rotavirus/isolation & purificationABSTRACT
Background: Yupkpa community in Perijá Sierra does not have drinking water or sewage elimination systems. Thus it becomes a propitious place for infection with intestinal parasites. Aim: To determine the prevalence of intestinal parasites in children, ages 0 to 14 years, in the Yukpa ethnic population of Toromo in the Perijá Mountains, Zulia State, Venezuela. Material and Methods: Ninety one fecal samples were analyzed by the following coproparasitologic methods: saline solution and lugol fresh mount, formalin-ether concentration (Ritchie), and Kinyoun stain for intestinal coccidians. Results: Overall parasite infection prevalence was 83.5%. The most frequently observed parasites were: Ascaris lumbricoides (57.1%), Trichuris trichiura (20.8%), Hymenolepis nana (14.2%), Blastocystis hominis (51.6%), Giardia lamblia (30.7%), and Entamoeba histolytica/E. dispar complex (21.9%). Coccidians were nont observed in any of the stool samples. Conclusions: The large number of infected people is directly related to the hygienic and sanitary conditions of the population studied.