ABSTRACT
Se examinaron 177 muestras de miel para la detección de Clostridium botulinum; 68 procedentes del comercio, 95 de apiarios envasados en origen, 8 de apiarios rurales para consumo familiar y 6 de establecimientos fraccionadores en las provincias de San Luis y Mendoza. Utilizando el método de dilución-centrifugación, se detectó C. botulinum tipo A en 2 muestras de apiarios rurales de San Luis (1,1 o/o); en una de ellas se logró la recuperación de la cepa y el recuento de esporas fue de 55/g de miel. Si bien el porcentaje de positividad fue inferior al de otros países, se recomienda no suministrar miel a niños menores de 1 año como prevención de botulismo del lactante
Subject(s)
Humans , Infant , Botulism/diagnosis , Clostridium botulinum/isolation & purification , Honey/analysis , Spores, Bacterial/isolation & purification , Botulinum Toxins/isolation & purificationSubject(s)
Humans , Female , Infant , Botulism/diagnosis , Feces/microbiology , Honey/adverse effects , Honey/microbiology , Muscle Hypotonia/etiologyABSTRACT
The bacteriological analysis and, particularly, the detection of Clostridium botulinum spores from 42 honey samples collected in apiaries of the province of San Luis as well as neighbouring areas of La Pampa, Córdoba and Mendoza, were carried out. Samples were processed by the dilution and centrifugation procedures. For spores detection, culture of the pellets were performed in 2 tubes with cooked meat medium (MCC), one of them warmed up to 80 degrees C for 15 min, and both incubated at 30 degrees C during 7 days. Mice were used to search for toxin in the supernatant. Sediments were also searched for anaerobic bacteria detection in yolk agar plates and in nutritive agar plates for the aerobics. Botulinum toxin type A production was found in one of the MCC cultures. No anaerobic bacteria were isolated. All samples contained Bacillus spp.; 21.4
of the strains, were tentatively classified as B. alvei. A working model for the bacteriological analysis of honey and guides that could be enclosed in publications of official institutions (Figure 1) is proposed.