ABSTRACT
El objetivo de este estudio fue identificar las especies de Candida spp. aisladas de secreción vaginal de pacientes embarazadas y no embarazadas y relacionarlas con la microscopía, síntomas y signos característicos de la vaginitis causada por esta levadura. Se estudiaron 743 muestras de secreción vaginal de pacientes que acudieron al Departamento de Bacteriología y Micología del Laboratorio Central en el 2015. Las muestras fueron sembradas en CHROM agar Candida y agar Sabouraud. La identificación se hizo por macro y micromorfología, pruebas bioquímicas, auxonograma y método comercial. En las 522 pacientes embarazadas se aislaron 536 Candida spp.: C. albicans 463 (86,4%), C. glabrata 46 (8,6%), C. krusei 9 (1,7%), C. parapsilosis 9 (1,7%), C. tropicalis 8 (1,5%), C. lusitaniae 1 (0,1%).En las 221 pacientes no embarazadasse aislaron 222 Candida spp.: C. albicans 163 (73,4%), C. glabrata 31 (14%), C. krusei 10 (4,6%), C. parapsilosis 9 (4,1%), C. tropicalis 6 (2,7%), C. guilliermondii 1 (0,4%), C. kefyr 1 (0,4%) y C. novergensis 1 (0,4%). Se observó un mayor porcentaje de aislamiento de Candida no albicansen las no embarazadas (26,6% vs 13,6%). En 15 pacientes (2%) se aislaron dos especies de Candida.Tanto en embarazadas como no embarazadas el prurito, la reacción inflamatoria y la presencia de pseudohifas fueron más frecuentes cuando el aislamiento era C. albicans. Enfatizamos la importancia de la siembra de las muestras en agar cromogénico para identificar y diferenciar especies de Candida para la epidemiología y un tratamiento eficazde la vaginitis causada por esta levadura.
The objective of this study was to identify Candida spp. isolated from vaginal secretion of pregnant and non-pregnant women and relate them with microscopy, symptoms and signs characteristic of vaginitis caused by this yeast. A total of 743 vaginal secretion samples wasstudied from patients consulting at the Department of Bacteriology and Mycology of the Central Laboratory in 2015. All samples were cultured on CHROM agar Candida and Sabouraud agar. The identification was made by macro and micromorphology, biochemical tests, auxonogram and commercial method. In pregnant patients (n = 522), 536 Candida spp. were isolated: C. albicans 463 (86.4%), C. glabrata 46 (8.6%), C. krusei 9 (1,7%), C. parapsilosis 9 (1.7%), C. tropicalis 8 (1.5%), C. lusitaniae 1 (0.1%). In no-pregnant patients (n = 221),222 Candida spp.were isolated: C. albicans 163 (73.4%), C. glabrata 31 (14%), C. krusei 10 (4.6%), C. parapsilosis 9 (4.1%), C. tropicalis 6 (2.7%), C. guilliermondii 1 (0.4%), C. kefyr 1 (0.4%) and C. novergensis 1 (0.4%).In the non-pregnant women, a higher percentage of non-albicans Candida species isolation was observed (26.6% vs 13.6%). Fifteen patients (2%) with two Candida species were detected. In pregnant as well non pregnant women, presence of pruritus, inflammatory reactions and and presence of pseudohifas were more frequent when Candida albicans was isolated.We emphasize the importance of culturing samples in chromogenic agar to identify and differentiate Candida species for epidemiology and an effective treatment of the vaginitis caused by this yeast.
Subject(s)
Female , Humans , Adolescent , Adult , Pregnancy , Middle Aged , Candidiasis, Vulvovaginal , Vaginal Discharge , InfectionsABSTRACT
Estudio observacional descriptivo de carácter retrospectivo, comprendido en el periodo 2006 al 2008 de todo los hemocultivos procesados en el Laboratorio Central de Salud Pública de Paraguay con aislamiento de Estafilococos Coagulasa-Negativos (ECN). El antibiograma se realizó empleando el método de Kirby-Bauer y siguiendo recomendaciones del Clinical and Laboratory Standards Institute (CLSI)
Subject(s)
Drug Resistance, Bacterial , Drug Resistance, Multiple, Bacterial , ParaguayABSTRACT
Reporte del aislamiento a partir de muestras clínicas de cepas de Klebsiella pneumonieas (KPN) resistentes a la carbapenems, que tiene como objetivo alertar a la comunidad sanitaria acerca de la presencia de las mismas en Paraguay
Subject(s)
Klebsiella pneumoniae , Klebsiella pneumoniae/immunology , Klebsiella pneumoniae/isolation & purification , ParaguayABSTRACT
Estudio realizado a 44 cepas para conocer la especie estafilococos coagulasa negativa (ECN) resistentes a la novobiocina
Subject(s)
Coagulase/isolation & purification , Coagulase/chemistry , Drug Resistance, Bacterial , Paraguay , Clinical Laboratory TechniquesABSTRACT
The presence of microbial organisms in conjunctival secretions always raises the doubt of whether they are the result of contamination or a real infection. Culture records of conjunctival secretion from children, who attended the Laboratory of Microbiology of the Instituto de Investigaciones en Ciencias de la Salud and the consulting roon of the Hospital de Clinícas from January 1990 to July 1992, were analyzed to determine the most frequent infectious agenis. The most commonly microorganism foud was Staphylococcus aureus (36.4 percent of the cases), followed by Streptococcus viridans (21.2 percent), Streptococcus pneumoniae (15 percent) pseudomonas aeruginosa (9 percent) and Neisseria gonorroheae (6 peecent). Nor Haemophilus influenzae neither fungi were detected
Subject(s)
Child , Haemophilus , Neisseria gonorrhoeae , Pseudomonas aeruginosa , Staphylococcus aureus , Streptococcus pneumoniae , ParaguayABSTRACT
A bacteriological study of drinking water in three peripheral neighborhood of Asunción was performed. the sample were taken at nine homes dwellers were asked a questionnaire. The sample were taken in sterile 1-liter bottles from: 1. Water kept in 50 lt-plastic buckests; 2. Directly from the tap. At the time of sample taking, several children have acute diarrhea, some of them with several days of duration. The sample were filtered through 0.45 mu Millipore membranes and the material retained on the filter was cultured on S.S agar medium and incubated at 37 ºc overnigth. On the other hand, 10 ml of the sample were cultured in alkaline peptone, pH 8.5, peptone broth and selenite borth and incubated at 37 ºc for 12 h. Subculture was performed in TCBS agar, EMB agar and Ss agar respectively. Biochemical characterization of the growing micoorganisms isolate were Salmonella ssp, Enterobacter ssp and Prosteus ssp. Six samples were negative for microorganisms
Subject(s)
Bacteriological Techniques , Enterobacter , Proteus , Salmonella , Drinking Water , ParaguayABSTRACT
In order to demonstrate the effectiveness of mixed rehydration (intravenous and oral) in children with acute diarrhea and showing dehydration, several physical parameters were meansured at the time of delivery to the Pediatrics Department of the Instituto de Previsión Social, and during the therapy. Substantial reduction in the dehydration rate were detecte by performing this method
Subject(s)
Diarrhea, Infantile , Fluid TherapyABSTRACT
An etiological study of bacterial diarrhea in infants under five years of age in Paraguay from May to July of 1990 was done. Enteropathogenic E. coli (EPEC) strains of known serogroups were isolate from 35.8 percent of the patients with diarrhea, Salmonella spp. were isolated from 15.1 percent , enterotoxigenic E. coli (ETEC) strains were isolated from 5.7 percent, and Shigella and Campylobacter spp. were isolated. Out of 53 diarrheal patients, 34 cases (64.1 percent) had bacterial diarrheal disease. The infants under one year of age with diarrhea were predominant among impatient and outpatient children under five years of age and the total number of male diarrheal cases was greater than that of female cases. Enteropathogenic organisms were isolated from nine out of 12 water samplestaken from the Paraguay River. The major serotype (O18) of the EPEC isolated from the water samples of the Paraguay River correspond to the mayor serotype isolated from patients with diarrhea
Subject(s)
Campylobacter , Child , Salmonella , Vibrio , ParaguayABSTRACT
The prevalence of Candida albicans, Neisseria Gonorrhoeae, and trichomonas vaginalis as some of the most important agents of sexually transmditted disease (STD), were studied in 100 women, belonging to a high risk group (protitues). Also a questionnaire were provided, in order to determine the potential risk of transmission of STD at the time of the survey. The prevalence of the infection by Candida albicans in the genital tract were 7 percent, and 8 percent and 10 percent for Neisseria gonorrhoeae and Trichomonas vaginalis, respectively. In some cases, inappropriate sexual behavior, as well as high number of sexual contacts with different clients, were detected
Subject(s)
Candida albicans , Sexually Transmitted Diseases/prevention & control , Neisseria gonorrhoeae , Trichomonas vaginalisABSTRACT
In order to determine the incidence and the etiology of the sexually trasnmitted diseaes (STD) 500 women, belonging to a high risk group (prostitutes), were studied from August, 1989 to Octuber 1990, and bacteriological and mycological studied were performed. Also, a questionnaire comprising detailed sexual behavior were rendered. High incidence of Gardnerella vaginalis (41.4 percent), Neisseria gonorrhoeae (12.2 percent), Trichomonas vaginalis (10.2 percent) and Candida albicans (10 percent) were found, in contrast with the low level found for Lactobacillus sp. The questinnaire revealed very low utilization of contraceptives as well as condom employement