ABSTRACT
Method development for the determination of major constituents from Khamin-chan, Curcuma longa Linn., which are curcumin (CI), demethoxycurcumin (CII) and bisdemethoxycurcumin (CIII) by high performance liquid chromatography (HPLC) has been developed. The reverse phase HPLC system consisted of a Dupont Zorbax ODS column, and 36% tetrahydrofuran (THF) in 10% glacial acetic acid as a mobile phase. The column effluent was monitored by a Jasco FP-210 Spectrofluorometer detector with Ex = 420 nm, and Em = 530 nm. The correlation of calibration curve of CI, CII and CIII were excellent with their r’s value 0.99 in all cases. The reproducibility study gave a very good results with the percent C.V. of about 5 percent or less for all three curcuminoids. With this analytical procedure, pharmacokinetic study of Khamin-chan in in situ model was undertaken. It was found that all three curcuminoids were poorly absorbed. About 60-70 percent of curcumins were absorbed within 30 minutes. While there was no statistical difference in the absorption rate between CI and CII, the poorest absorption compound was found to be CIII.