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Article in English | IMSEAR | ID: sea-138134

ABSTRACT

For determination of albumin in urine, Bromcresol Green (BCG) was used as follows: stock BCG regent was diluted to 1:20, and 2.5 ml. of the reagent was added to 100 µl of specimen; the resulting reaction mixture was measured for optical density at 630 nm. The values of the routine Rebert’s Test and reagent strip (Albustix) were compared to those produced by the BCG method. The mean albumin values of the BCG method were 22.4, 54.6, 95.6, 180.4, 396.3 and 1,176.3 mg/dl which were comparable to the negative, trace, 1+, 2+, 3+, 4+ values of the Robert’s Test, respectively. The mean albumin values of the BCG method were 26.3, 52.7, 96.3, 191.7, 476.1 and 1,273.1 mg/dl which were comparable to the negative, trace, 1+, 2+, 3+, 4+ values of the Albustix, respectively. The albumin values from Albusix and the Robert’s Test could be estimated from this study.

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