ABSTRACT
We report the case of a 52-year-old patient with type 2 diabetes mellitus diagnosed with adenocarcinoma rectum, presenting with the complaint of breathlessness to the emergency department. Chest radiograph done showed a left-sided hydropneumothorax with mediastinal shift to the right side. Tube thoracostomy was done. Pleural fluid was exudative; there was no evidence of malignancy. The patient developed a right-sided pleural effusion; anaerobic bacteria were grown on pleural fluid culture. Computed tomography (CT) of the chest was done in view of the new onset empyema on the right side. The CT showed pneumomediastinum and periesophageal air pockets. Orally administered methylene blue had appeared in the pleural drain confirming the diagnosis. The patient was taken up for feeding jejunostomy and repair of tear was planned for a later date. He succumbed to sepsis due to empyema.
ABSTRACT
Thorium (IV) forms a yellow Coloured water soluble complex with Diacetyl Monoxime Isonicotinoyl hydrazone (DMIH) reagent in acidic buffer of PH 5.0 with λ max at 352 nm. The molar absorptivity and sandell’s sensitivity are 2.265 X 10 4 L mol -1 cm -1 and 0.0022μg /cm2 respectively. The Beer’s law validity range 1.16–13.92 μg / mL. Thorium (IV) forms (M: L) 1:1 complex with DMIH and stability constant of the complex is 7.0265 X 10 6.The derivative spectrophotometric determination of Th (IV)) was carried out by measuring peak height method. The developed derivative spectrophotometric method was employed for the determination of Thorium (IV) in synthetic samples of alloy. The effect of various diverse ions was also studied.
ABSTRACT
A simple and sensitive spectrophotometric method has been developed for the determination of Gold (III) using 3,5-Dimethoxy-4-hydroxy benzaldehyde benzoylhydrazone (DMBBH) in a neutral surfactant of Triton X-100(5%) (micellar medium). Gold (III) forms an wine red coloured water soluble complex with the reagent in acidic medium pH 5.0. The molar absorptivity and Sandell’s sensitivity of coloured species are 2.32 x 104 L.mol-1 cm-1 and 0.00862 mg/cm2 respectively. Beer’s law is obeyed in the range 0.4925-5.91 mg/ml of Au(III) at lmax at 380 nm. The developed derivative spectrophotometric method was employed for the determination of gold (III). This method has been satisfactorily applied for the determination of gold (III) in water samples and pharmaceutical samples.
ABSTRACT
A rapid, simple, sensitive and selective spectrophotometric method has been developed for the determination of Palladium (II) using newly synthesized reagent 4-Hdroxy3,5dimethoxybenzaldehyde-4- hydroxybenzoylhydrazone (HDMBHBH) in neutral surfactant of TritonX-100-5% (micellar medium). Palladium (II) forms a brown coluored water-soluble complex with 4-Hdroxy 3, 5dimethoxybenzaldehyde-4- hydroxybenzoylhydrazone in the pH range 1.0-6.0. The complex shows maximum absorbance at lmax 373 nm and in the pH range 3.0-4.0. However, at this wavelength, the reagent shows considerable absorbance. At lmax 373 nm, the complex shows maximum absorbance while the reagent blank shows negligible absorbance. Hence, analytical studies are carried out at lmax 373 nm and at pH 3.0 (Phosphate buffer) against reagent blank. Beer's law is obeyed in the range 0.106-1.064 μg ml-1 and the optimum concentration range from ringbom plot is 0.212-0.957 mg/ml of Palladium (II). The molar absorptivity and Sandell's sensitivity for the coloured solution are found to be 7.5 x 104L mol-1 cm-1 and 0.0015-μg. cm-2 respectively. The interference effect of various diverse ions has been studied. The complex shows 1:1 [Pd (II): HDMBHBH] stoichiometry with stability constant 7.29 x 106. The standard deviation of the method in the determination of 0.638 - μg ml-1 of Palladium (II) is 0.003 and the Relative standard deviation is 0.71%. First and second order derivative spectroscopic method is developed at lmax 422 nm and lmax 444 nm respectively for the determination of Palladium (II), which is more sensitive than the zero order method. The developed method has been employed for the determination of Palladium (II) in hydrogenation catalyst samples and in synthetic alloy samples. The results are in good agreement with the certified values.
ABSTRACT
In recent decades studies on RNA structure and function have gained significance due to discoveries on diversified functions of RNA. A common element for RNA secondary structure formed by series of non- Watson/Watson Crick base pairs, internal loops and pseudoknots have been the highlighting feature of recent structural determination of RNAs. The recent crystal structure of group-I introns has demonstrated that these might constitute RNA structural motifs in ribozymes, playing a crucial role in their enzymatic activity. To understand the functional significance of these non-canonical base pairs in catalytic RNA, we analysed the sequences of group-I introns from nuclear genes. The results suggest that they might form the building blocks of folded RNA motifs which are crucial to the catalytic activity of the ribozyme. The conservation of these, as observed from divergent organisms, argues for the presence of non-canonical base pairs as an important requisite for the structure and enzymatic property of ribozymes by enabling them to carry out functions such as replication, polymerase activity etc. in primordial conditions in the absence of proteins.
Subject(s)
Animals , Base Composition , Base Pairing , Introns , Molecular Sequence Data , Nucleic Acid Conformation , RNA, Catalytic/genetics , RNA, Ribosomal/chemistryABSTRACT
We report a 45-year-old man and a 60-year-old woman who presented with features of intermittent intestinal obstruction. Barium enema revealed narrowing at the pelvic-rectal junction in the man, and from the pelvic colon to the anal verge in the woman. Histology of the resected sections showed marked hypertrophy of the muscularis propria in both cases, with normal mucosa, submucosa and myenteric plexus. Both patients are asymptomatic at 4 years' and 2 years' follow up. This entity of primary hypertrophic colonopathy may be a variant of primary visceral myopathy.
Subject(s)
Anastomosis, Surgical , Colectomy/methods , Colonic Diseases/diagnosis , Female , Follow-Up Studies , Humans , Hypertrophy/complications , Intestinal Mucosa/pathology , Intestinal Pseudo-Obstruction/diagnosis , Laparotomy , Male , Middle Aged , Rectal Diseases/diagnosis , Treatment OutcomeABSTRACT
Waldenstrom's macroglobulinaemia is a rare form of monoclonal plasma cell dyscrasia in which there is excess production of immunoglobulin M. Clinically the patient has features of hyperviscosity syndrome and the effects of bone marrow replacement by lympho-plasmacytoid cells. We report here two such cases seen within a period of two months.