Structural equation model analysis of mental health influencing factors in family caregivers of schizophrenic patients / 中华疾病控制杂志

@# Objective The structural equation model was used to analyze the influencing factors of mental health of family caregivers with schizophrenia, so as to provide scientific basis for targeted intervention measures. Methods On the basis of investigating the social dysfunction of schizophrenia patients and their family function, social support and mental health status, structural equation model was used to analyze the factors affecting mental health of caregivers and their effect indexes, the main fitting parameters included compare the fitted indices(CFI), goodness of fit index (GFI), the adjusted goodness of fit index(AGFI), normed fit index(NFI) and approximate error root mean square(RMSEA). Result The total score of psychological self-rating scale of carers was higher than that of normal people(all P<0.05). Only 19.0% of home care providers with schizophrenia felt no or little burden, 33.0% mild burden and 48.0% heavy burden. Structural equation model fitting index for: 2(58)=47.440, P=0.838, 2/df=0.818, the main fitting parameters GFI=0.968、AGFI=0.943, CFI=1.000, NFI=0.973, RMSEA=0.000, model fitting effect was good. The total effects of social support, family care, patient social function and care burden on the mental health of family caregivers were -0.016, -0.197, 0.114 and 0.118, respectively. Conclusions The family caregivers of schizophrenia have serious mental health problems, and the burden of care is heavy. improving social support, family care and social function of patients is an effective way to improve their care burden and mental health level.

Content of neutrophil elastase in EPS and seminal plasma: A combined predictor in the diagnosis of type IIIA prostatitis with secondary infertility / 中华男科学杂志

Objective@#To investigate the value of the content of neutrophil elastase (NE) in the expressed prostatic secretion (EPS) and seminal plasma (SP) as a combined predictor in the diagnosis of type IIIA prostatitis with secondary infertility.@*METHODS@#This study included 62 fathers (group A) and 67 infertile men (group B), all with type IIIA prostatitis, and another 57 controls with no genitourinary tract disease (group C). We measured the NE contents in the EPS and SP, obtained the results of routine semen and EPS examinations and Chronic Prostatitis Symptom Index (CPSI), and calculated the ratio of EPS NE/SP NE by binary logistic regression analysis.@*RESULTS@#The combined predictor of type IIIA prostatitis with secondary infertility was SP NE-2 × EPS NE. Among the 129 patients with type IIIA prostatitis, the combined predictor was correlated strongly negatively with the WBC count in the EPS (r = －0.914, P 0.05). The mean values of the combined predictor in groups A, B, and C were －2 238 (95% CI: －2 595 to －2 054), －1 511 (95% CI: －1 778 to －1 307), and －148 (95% CI: －181 to －118), respectively, with statistically significant differences between the cases and controls as well as between groups A and B (P <0.01). The area under the ROC curve of the combined predictor for the diagnosis of type IIIA prostatitis with secondary infertility was 0.71 (P <0.001).@*CONCLUSIONS@#The content of neutrophil elastase in the EPS combined with that in the seminal plasma contributes to the diagnosis of type IIIA prostatitis with secondary infertility, which is superior to either the neutrophil elastase content in the EPS or that in the seminal plasma used alone.

Study on the standard of health protection zone for petroleum processing industry / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To establish health protection zone standards for petroleum processing industry.</p><p><b>METHODS</b>The intensity of characteristic pollutants from fugitive emission were estimated by the inverse method of ground concentration through field survey and monitoring for representative petroleum processing industry, which was calculated health protection zone by using the model of atmospheric diffusion.</p><p><b>RESULTS</b>Characteristic pollutant of fugitive emission source from petroleum processing industry was confirmed as hydrogen sulfide. When local average wind speed in the past five years was less than 2, 2-4 m/s and more than 4 m/s respectively and meanwhile the scale of petroleum processing industry was less than 8 million tons, the recommended value of health protection zone were 900, 800, 700 m respectively. Besides, when the scale of petroleum processing industry was more than 8 million tons and in the same wind speed level, the recommended value of health protection zone were 1200, 1000, 900 m respectively.</p><p><b>CONCLUSIONS</b>Recommended value of health protection zone for petroleum processing industry was reasonable and feasible through revising and improving of the version of 1987's standard.</p>

Effect of NS-398 on cyclooxygenase-2 expression and proliferation of HepG2 cells / 中华预防医学杂志

<p><b>OBJECTIVE</b>To investigate anticancer effect and molecular mechanism of N-[(Cyclohexyloxy)-4-nitrophenyl] methanesulfonamide on HepG2 cells in vitro.</p><p><b>METHODS</b>HepG2 cells were treated with various concentrations (100, 200, 300, 400 micromol/L) of NS-398 [selective for cyclooxygenase 2 (COX-2) inhibition]. Cell growth was measured by MTT method, DNA fragmentation gel analysis was used to analyze the apoptosis cells, DNA ploidy and apoptotic cell percentage were examined by flow cytometry (FCM). PGE2 concentration was measured by radioimmunoassay method. The expressions of COX-2 were also examined by Western blot analysis.</p><p><b>RESULTS</b>NS-398 inhibited HepG2 cell proliferation and induced apoptosis in a concentration-dependent manner. DNA ploidy analysis showed that S phase cells were significantly decreased and quiescent G1 phase was accumulated with NS-398 concentration increasing. The IC50 of 24 hours was 300 micromol/L. The release of PGE2 was significantly reduced in HepG2 cells with the values of NS-398 being (0.70 +/- 0.02), (0.48 +/- 0.02), (0.29 +/- 0.01) and (0.18 +/- 0.01) respectively, as compared with control group (0.03 +/- 0.01). NS-398 could inhibit the activity and expression of COX-2, with higher concentration, it can significantly down-regulate the expression of COX-2 (t = 3.736, 1.623, 1.810, 2.587, P < 0.01).</p><p><b>CONCLUSION</b>NS-398 might significantly inhibit the proliferation of HepG2 cells and induce apoptosis. The mechanisms were related with the accumulation of quiescent G1 phase and the inhibition of COX-2 activity.</p>

Inhibition of curcumin on histone deacetylase and expression promotion of P21 (WAF1/CIP1) in HepG2 cells / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effect of curcumin (Cur) on histone deacetylase (HDAC1) and P21(WAF1/CIP1), a cyclin dependent kinase inhibitor, in HepG2 cells for exploring the mechanism of Cur in anti-cancer.</p><p><b>METHOD</b>The HDAC1, P21(WAF1/CIP1) proteins and P21(WAF1/CIP1) mRNA were extracted from human hepatoma cells treated with or without Cur of different concentrations at different time points. Western blot analysis was performed to determine the levels of HDAC1 and P21(WAF1/CIP1) proteins, respectively. RT-PCR was performed to detect the level of P21(WAF1/CIP1) mRNA.</p><p><b>RESULT</b>The IC50 of concentration treated by Cur was 25 micromol x L(1) on HepG2 cell. The level of HDAC1 was obviously inhibited by Cur, and decreased at 4 hours at IC, and lasted for 48 h in a time-dependent manner. The inhibition of HDAC1 was significant at the Cur concentration of 12.5 micromol x L(-1) but there was no difference between 50 and 100 micromol x L(-1). The levels of P21(WAF1/CIP1) mRNA and protein were up-regulated by Cur in dose and time-dependent manner, and the change of mRNA and protein was detected at 8 hours and lasted for 48 hours.</p><p><b>CONCLUSION</b>Cur can inhibit the level of HDAC1 and enhance the expression of P21(WAF1/CIP1) protein and mRNA, and the results suggest that inhibiting HDAC1 and increasing P21(WAF1/CIP1) may be one of the possible mechanisms of anti-cancer by Cur.</p>