ABSTRACT
Objective: To establish HPLC method for the determination of loureirin A and loureirin B in Sanguis Draxonis Capsules.Methods: HPLC system included C 18 reverse phase column and acetonitrile 1% acetic acid (34∶66) as mobile phase, detection at 280nm and external standard method.Results:The standard curves of loureirin A was linear in the concentration range of 98～490ng, r =0.9996.The average recovery was 97.45%, RSD was 1.82%.The standard curves of loureirin B was linear in the concentration range of 43.2～259.2, r = 0.9993 .The average recovery was 96.92%, RSD was 1.57%.The contents of loureirin A and loureirin B in Sanguis Draxonis from heating free technology were higher than traditional technology.Conclusion: This heating free technology is a new technique that is a creation with higher extract rate,this method is worth populariging.
ABSTRACT
Objective: To establish HPLC method for determination of pterostilbene in Dragon's Blood with different extraction technology. Method: Applying a C 18 phase column and acetonitrile 1% acetic acid(41∶59) as mobile phase, detecting at 319nm and quantitating with external standard method. Results: The standard curves of pterostilbene was linear in the concentration range of 10.4～104ng, r =0.9992. The average recovery was 98.28%, RSD =1.97%. The content of pterostilbene in Dragon's Blood extracted with ordinary temperature was lower than that with heating technology. Conclusion: The content of pterostilbene extracted with ordinary temperature extraction in Dragon's blood have the advantage of heating extraction.