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Objective To explore the role of macrophage migration inhibitory factor (MIF) in the pathogenesis of pathological scar and the effect of ISO-1 on the behavior of scar fibroblasts.Methods Samples of normal skin,normal scar,and pathological scar were collected and detected by hematoxylin-eosin staining and immumohistochemical staining.Human fibroblasts were isolated from the samples and then divided into different groups with the intervention with ISO-1 (0 ～ 100 μ mol/mL).Fibroblast proliferation was detected by Alamber dyeing and cell apoptosis was detected by TUNEL staining.Expressions of fibroblast specific proteins and PI3K/Akt/mTOR signaling pathways wcre detected by Western Blot and RT-PCR.Results The positive rates of MIF for hyperplastic scar and keloid were greater than those for normal scar and normal skin (P ＜ 0.01).Apoptotic cells occurred less in the group without intervention.The apoptotic rate increased gradually as the concentration of ISO-1 increased.There were significant statistical differences in the migration rate among all the groups (P ＜ 0.05).As concentration of ISO-1 increased,the protein and gene expressions of type I collagen,FN and CTGF were decreased.Expressions of activated PI3K and Akt decreased as ISO-1 concentration increased.Conclusions The expression of MIF is different in different types of scar tissue.ISO-1 inhibits the biological behavior of fibroblasts derived from pathological scar through PI3K/Akt/mTOR pathways.
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Objective To explore the clinical effect of subcutaneous negative pressure suction cosmetic suture with anti L incision in liver surgery.Methods A total of 140 patients who received liver surgery in general surgery department of our hospital from March 2015 to April 2016 were randomly selected,of which 70 cases received cosmetic suture with negative pressure drainage (observation group),the other 70 cases treated with conventional suture(control group).The repair times,the healing of the incision of two groups were observed and analyzed.Results Compared with the control group,the repair time and hospital stays of patients in observation group were significantly shorter than those of the control group,and the differences were statistically significant (P ＜ 0.01).Conclusion Cosmetic suture with negative pressure drainage is effective to shorten the repair time,reduce the incidence of incision fat liquefaction and infection,while ensuring the aesthetic level of the incision.
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BACKGROUND:Autologous split-thickness skin grafting is the main therapy for burn repair at functional sites, which has achieved certain effects, but there are stil some deficiencies, such as poor texture, stiffness and poor toughness, as wel as severer hyperplasia that is easy to result in contracture deformity and poor functional recovery. OBJECTIVE: To analyze the clinical efficacy of skin co-transplantation on burn repair at functional sites. METHODS:Sixty patients with burns at functional sites (n=84) were randomized into two groups: co-transplantation of acelular dermis and autologous split-thickness skin in experimental group and autologous split-thickness skin graft in control group. Survival rate of skin flap and rate of secondary operation were compared between two groups. At 1 month after transplantation, Vancouver Scar Scale was used to assess skin color, thickness, blood vessel distribution and flexibility, and meanwhile, the severity of scar was determined. RESULTS AND CONCLUSION:The survival rate of skin flap was significantly higher in the experimental group than the control group (93%vs. 70%,P < 0.05), and the rate of secondary operation was significantly lower in the experimental group compared with the control group (0vs. 13%,P < 0.05). At 1 month after transplantation, scores on the skin color, thickness, blood vessel distribution and flexibility were al lower in the experimental group than the control group (P < 0.05), but the incidence of mild hyperplasia in the experimental group was significantly higher than that in the control group (52% vs. 29%,P < 0.05). These findings indicate that co-transplantation of acelular alogeneic dermis and autologous split-thickness skin for burn repair at functional sites can effectively enhance the survival rate of skin flap, reduce the rate of secondary operation, contribute to wound healing and reduce the severity of hyperplasia.
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Objective To determine the significance of thymidine phosphorylase for prognosis of HCC.Methods The clinical data and pathological finding of 65 patients with HCC treated in our hospital were retrospectively analyzed.Immunohistochemical staining was performed to evaluate the expression of TP and MVD count.Statistical analysis was used to analyze the relationship between TP expression and other pathological parameters.Survival curves in 54 patients were plotted using the Kaplan-Meier method.Results The positive expressed rate of HCC tissues was significantly higher than that of normal liver tissues (P<0.01).MVD count in positive-TP group and negative-TP group was respectively 16.14±5.11,10.11±4.55.The difference between two groups had statistical significance (P<0.01).TP expression was positively correlated with tumor size,Edmondson-Steiner grade,tumor capsule status,microsatellite marker and tumor thrombi in portal vein (TTPV).The rate of postoperational recurrence after one,two and three years in TP-positive group was respectively 40%,66.7%,79.2% and it was respectively 27.4%,31.3% and 45.7% in TP-negative group.The postoperative recurrence rate after two years and three years in poitive-TP group was significantly higher than that in negative-TP group (P<0.01).In 54 patients with HCC followed up successfully,the total survival rate in TP-positive group was significantly lower than in TP-negative group (P<0.01).Conclusion TP plays an important role in neovascularization of HCC and TP may be a new prognosis index of HCC.