Exploration and practice of experimental skills training for young teachers of basic medical science / 中华医学教育探索杂志

With basic medicine teaching faculty becoming younger,it is still in accordance with the traditional training mode about training the young teachers' experimental skills, which brings about some problems such as nonstandard basic operation, weak basic knowledge, lack of systematic skills training and relative lack of research.In practice,we strengthen the basic skills of young teachers through skills training and assessment, focus on the cultivation of young teachers' basic quality by paying attention to theoretical teaching skills and experimental skills; guide teachers to emphasize experimental skills through holding ex-perimental skills competition; promote teachers' experimental skills by integrating the subject research into experiment practice which proved that the experimental skills and teaching level of young teachers get sig-nificantly improved.

Effect of bacailin on the angiogenic regulating factor and migration of laryngeal cancer cells / 中国医师杂志

Objective To explore the effects of bacailin on the angiogenic regulating factor and migration activity of laryngeal cancer cells.Methods Hep-2 was cultured in vitro,(1)in the groups adding phorbol ester,gelatin-zymogram and Transwell assay were used to observe the effects of bacailin on the gelatinase activity of matrix metalloproteinase (MMP) and the migration ability of Hep-2,respectively; (2)in the group without phorbol ester,enzyme-linked immunosorbent assay (ELISA) was used to detect the effects of bacailin on the protein level of endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF).Results Among the control group,the low-dose group,the middle-dose group,and the high-dose group,the activity of MMP-3 of each corresponding group was (100.0 ±0.0) ％,(95.0 ±4.9) ％,(93.1± 6.2)％,and (90.7 ± 7.3)％ with statistically significant difference (t =2.123,2.309,2.412,P ＜0.05) ; the activity of MMP-9 of each corresponding group was (100.0 ± 0.0) ％,(92.3 ± 7.5) ％,(89.5 ± 9.3) ％,and (85.6 ± 6.1) ％ with statistically significant difference (t =2.253,2.302,3.708,P ＜0.05 or P ＜0.01) ;the protein level of VEGF of each corresponding group was (242.7 ±9.5)ng/L,(230.6 ± 12.7)ng/L,(212.1 ± 15.9)ng/L,and (184.2 ± 23.5)ng/L with statistically significant difference (t =2.408,3.733,4.146,P ＜0.05 or P ＜0.01) ; the protein level of bFGF of each corresponding group was (190.7±8.2) ng/L,(181.2±13.0) ng/L,(169.9±17.3)ng/L,and (140.7±9.2)ng/Lwith statistically significant difference (t =2.330,2.922,5.145,P ＜0.05 or P ＜0.01) ; Cellular migration rate of each corresponding group was (38.3 ± 5.6) ％,(32.9 ± 7.1) ％,(27.9 ± 4.4) ％,and (23.3 ± 6.0) ％ with statistically significant difference (t =2.141,3.365,4.027,P ＜ 0.05 or P ＜0.01).Conclusions Bacailin could suppress the migration of Hep-2 through inhibiting expression of angiogenic regulating factor,which might has clinical value in the treatment of laryngeal cancer.

Endoscopic orbital decompression for thyroid-associated ophthalmopathy / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the therapeutic results of endoscopic orbital decompression for thyroid-associated ophthalmopathy.@*METHOD@#The records of nine patients (twelve orbits) received endoscopic orbital decompression for thyroid-associated ophthalmopathy were analyzed for changes in visual acuity, intraocular pressure, proptosis, corneal ulceration and movement. The follow-ups ranged from two months to thirty-six months.@*RESULT@#Twelve orbits (100%) had improvement in visual acuity (range 0.1-0.7). Ten orbits (83.3%) decreased in intraocular pressure (range 0.2-21.4 mm Hg). Eight orbits (66.70%) decreased in proptosis (one-five mm). The orbit with corneal ulcer was healed after decompression. Diplopia was cured in one of four patients.@*CONCLUSION@#Endoscopic orbital decompression is a safe and effective procedure for the treatment of thyroid-associated ophthalmopathy.

Analysis on clinical characteristics, treatments and prognostic factors of head and neck non-Hodgkin's lymphoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the clinical characteristics, treatments and prognoses of the primary non-Hodgkin's lymphoma (NHL) in head and neck.@*METHOD@#Clinical manifestations and clinicopathology characteristics of 107 NHL patients in head and neck were analyzed retrospectively.@*RESULT@#The median age of patients with head and neck NHL was 54 years. The most commonly primary site was tonsil (29 cases, 27.10%), and the secondly primary site was nasal cavity (28 cases, 26.17%). The most common histologic subtype was diffuse large B cell lymphoma(46 cases, 42.99%), and the secondly one was NK/T cell lymphoma(24 cases, 22.43%). We found that the combination of rituximab was superior than that of chemotherapy alone. Prognosis depended on both IPI and histological subtype, and IPI was a more dangerous factor than histological subtype.@*CONCLUSION@#The primary NHL is a common neoplasm in the head and neck region. Characteristic in age, primary site, histologic subtype, treatment and prognostic factors were helpful to understand and treat non-Hodgkin's lymphoma in head and neck.

The association between genetic polymorphisms of IL-6 and the susceptibility of chronic rhinosinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the relationship between the promoter polymorphism of IL6 (-174G > C, -572G > C and -597G > A) and chronic rhinosinusitis (CRS).@*METHOD@#The case-control study consisted of 123 patients with CRS and 239 controls from a Chinese Han population from Shanghai. The genotypes of the subjects were determined by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. Besides, the concentrations of the totle immunoglobulin E (TIgE) and eosinophilic cationic protein (ECP) in the blood were also determined.@*RESULT@#The -174G > C and -597G > A polymorphisms were not detected in this study population. Significant differences in genotype and allele frequencies of -572C/G were observed between CRS patients and control groups. In CRS patients, the CC, CG, GG genotype frequencies were 69.1%, 29.3%, 1.6%, C, G allele frequencies were 83.7%, 16.3%. In control group, the genotype frequencies were 55.2%, 42.3%, 2.5%, the allele frequencies were 76.4%, 23.6%, respectively. The -572CC genotype was associated with an increased risk of developing CRS (P C polymorphism is associated with the susceptibility to CRS. CC genotype could be an independent risk factor.

Association of IL-1beta gene -31T/C polymorphism with chronic rhinosinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the relationship between polymorphism of IL-1beta gene 31T/C and chronic rhinosinusitis.@*METHOD@#One hundred and twenty-three patients with chronic rhinosinusitis and 239 healthy controls were collected to determine the genotypes by polymerase chain reaction-restriction fragment length polymorphism.@*RESULT@#There was significant difference in genotype and allelic frequencies between the CRS and control group (P 0.05).@*CONCLUSION@#IL-1beta gene 31CC genotype may be an independent risk factor with chronic rhinosinusitis.

Nasal anatomic abnormalities and bacterial infection in chronic rhino sinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the relationship between nasal anatomic abnormalities and the bacteria infection status of maxillary sinus.@*METHOD@#The anatomic abnormalities of 115 cases of maxillary sinuses were detected with the CT images and confirmed with chronic infection, which were divided into two groups: high anatomic abnormality group and low anatomic abnormality group. The sinusal contents were sent to bacteria culture, compare the bacteria infection rate and the distribution of bacteria between the two groups.@*RESULT@#The bacteria positive rate of the high anatomic abnormality group and low anatomic abnormality group was 90.32% and 56.60% (P < 0.01) respectively. The contribution of gram-positive bacteria and gram-negative bacteria are 47.76% and 52.24% in high anatomic abnormality group, 62.16% and 37.84% in low anatomic abnormality group.@*CONCLUSION@#Nasal anatomic abnormalities can improve the bacteria infectious rate of maxillary sinuses. High anatomic abnormality may more induces gram-negative bacteria infections,while low anatomic abnormality may more induces gram-positive bacteria infections.

Effect of allergy on outcomes after endoscopic sinus surgery in patients with chronic rhinosinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore correlation of allergy and outcomes after endoscopic sinus surgery (ESS) in patients with chronic rhinosinusitis.@*METHOD@#Before ESS, 115 cases were arranged to detect allergic serum relative index: the concentration of serum TIgE, specific IgE semi-quantitative test and the concentration of serum ECP; to survey QOL status: VAS, SNOT-20 and SF-36; as well as inquire medical record, Lund-Mackay CT system scoring, clinical classification of chronic rhinosinusitis. After ESS (>1 year), 74 cases were followed up. The subjective and objective assessment were achieved by means of VAS, SNOT-20, SF-36 and Lund-Kennedy endoscopic scores.@*RESULT@#The improvements of VAS scores of the cases with increased serum TIgE were significantly lower than those without increased serum TIgE (P or = 3) were significantly worse than those with positive allergen (<3) (P<0.05). The objective outcomes of the cases with strong positive allergen were significantly worse than those without strong positive allergen (P<0.05). VAS scores and SNOT-20 scores after endoscopic sinus surgery of the cases with strong positive allergen were significantly worse than those without strong positive allergen (P<0.05). The concentration of serum ECP of the invalid cases was significantly higher than effective cases (P<0.05). The concentration of serum ECP was positive correlated with Lund-Kennedy endoscopic scores (r=0.49, P<0.05). In cases of II, III types, the objective outcomes of the cases with increased serum ECP were significantly worse than those without increased serum ECP (P<0.05), VAS scores and SNOT-20 scores after endoscopic sinus surgery of the cases with increased serum ECP were significantly worse than those without incre- ased serum ECP (P<0.05), the improvements of VAS scores of the cases with increased serum ECP were significantly lower than those without increased serum ECP (P<0.05).@*CONCLUSION@#Those allergic serum relative indexes make some negative effect on subjective and objective outcomes. Especially, in cases with polyps, the increase of serum TIgE and serum ECP has more negative effect. Our results might help to choose immunotherapy to combine with endoscopic sinus surgery for those selected patients to improve the outcomes of chronic rhinosinusitis.

A computational fluid dynamics study of inner flow through nasal cavity with unilateral hypertrophic inferior turbinate / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the anatomical influence of the hypertrophic inferior turbinate on computational fluid dynamics (CFD) model of unilateral hypertrophic inferior turbinate nasal cavity, and to analyze the bilateral detailed nasal airflow simulations under both inspiratory and expiratory phases in CFD model.@*METHOD@#One male volunteer troubled with unilateral hypertrophic inferior turbinate accepted CT scan. CFD model was built by CT scans through Simplant 10.0 and ANSYS ICEM. Fluent 6.3.26 simulated the airflow of both nasal cavity in breathing rates 200 ml/s.@*RESULT@#1) In infraturbinal region, the cross-section area (CSA) of the nasal cavity with hypertrophic inferior turbinate was smaller than that in healthy side and the average area difference between two sides was 1.62 cm2. 2) In both inspiration and expiration phases, the hypertrophic infraturbinal produced a markable reduction in intranasal pressures drop along the full length of the infraturbinal region. The volumetric flow rate in the hypertrophic infraturbinal side was 50 ml/s, which equalled to one third of that in healthy side; Mean air speed in the anterior valve region was estimated to be 0.57 m/s at hypertrophic infraturbinal side and 1.83 m/s at healthy side during inspiration; More vortices happened in the hypertrophic infraturbinal side.@*CONCLUSION@#The unilateral hypertrophic infraturbinal change the normal anatomy and influence the aerodynamic of nasal cavity, which is harmful to the functions of human nasal in ventilation, temperature accommodation and olfactory sensation.

Effect of allergic factors on chronic rhinosinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the correlation between allergic factors and chronic rhinosinusitis, and discuss its effects on the development of illness.@*METHOD@#One hundred and one cases were selected to detect allergic serum relative index: the concentration of serum TIgE, specific IgE semi-quantitative test, the concentration of serum ECP as well as inquiring medical record, Lund-Mackay CT system scoring.@*RESULT@#The concentration of serum TIgE and serum ECP in CRS was higher than those in control (P < 0.01). The increased proportion of serum TIgE and serum ECP was significantly different among clinical stages (P < 0.05) and between the cases with and without prior sinus surgery (P < 0.05). CT scores of the cases with serum TIgE and serum ECP increased were significantly higher than those without serum TIgE and serum ECP increased. The strong positive rate in CRS was significantly different among clinical stages (P < 0.05). The strong positive rate increased significantly in the cases with prior sinus surgery (P < 0.05). CT scores in the cases with strong positive allergen were significantly higher than that in the cases without positive allergen (P < 0.05).@*CONCLUSION@#These results suggest that allergic factors make some negative effect on the severity of CRS and are the index for the severity and worse prognosis of illness.

Domestic Cetirizine Tablet in Treating Perennial Allergic Rhinitis / 中国药房

0 05) CONCLUSION:The clinical efficacy and adverse reaction of domestic cetirizine tablet are similar to that of imported one in treatment of PAR