ABSTRACT
Objective To investigate the effect of transarterial infusion ( TAI) chemotherapy on estrogen receptor ( ER) and progesterone receptors ( PR) expression in breast carcinoma tissue. Methods Samples of 81 patients with breast carcinoma were selected in this study. ER and PR mRNAs levels in the samples were determined by RT-PCR. Immunohistochemistry was employed to measure ER and PR expressions in different samples before and after TAI chemotherapy. Western blotting was used to assay ER and PR proteins level in the samples before and after the chemotherapy. Results RT-PCR results indicated that ER mRNA level was increased in 43 cases ( 53. 1% ) and PR level in 33 cases ( 40. 7% ) . Meanwhile,both ER and PR mRNAs levels were increased in 25 cases ( 30. 9% ) . Immunohistochemistry results showed that ER level was up-regulated in 35cases ( 43. 2% ) ,and PR level in 21 cases ( 26. 0% ) . Both ER level and PR level were up-regulated in 16 out of 81 cases( 19. 8% ) . Western blotting also suggested that the both proteins levels of ER and PR were up-regulated in some patients after the chemotherapy. Conclusion Transarterial infusion neoadjuvant chemotherapy affects the mRNA and protein level of the hormone receptors,which facilitates the subsequent killing effect of chemotherapy on tumor cells. ER and PR expressions should be determined after the transarterial infusion chemotherapy,which might provide a guide for the subsequent treatment and prognosis.
ABSTRACT
Objective:To investigate the effect of interventional therapy in patients with advanced breast cancer.Methods:According to Seldinger method, catheter was put into the opening of subclavian artery and thoracic internal artery through subcutaneous femoral artery puncture, then anti-cancer agents were given and recent response of the lumps was observed.Results:Thirty-one cases of advanced breast cancer received interventional therapy.Complete response (CR) was 9.68% (3/31),partial response (PR) was 83.87%(22/31),and total response rate RR(CR+PR) was 93.55%.Conclusion:Interventional therapy is effective for treating local advanced breast cancer.
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Objective:To study the effect of different dosage of tamoxifen (TAM) on the morphological change of experimental mammary hyperplasia.Methods:The animal model of mammary hyperplasia of Wistar rats was induced with estradiol and progesterone. One hundred and eighty female Wistar rats were selected randomly into group A (control group ) , group B (model control group), group C (166.7 ?g dosage of TAM),group D(333?g dosage of TAM) ,group E(500?g dosage of TAM),and group F(667?g dosage of TAM) and fed for 2 months.Samples of breasts were collected and observed under light microscope and electronmicroscope.Results:For light microscope observation :there were obvious difference between premedication and postmedication in group E and F, respectively ( P 0. 05), implying that 500 ?g dosage and 667 ?g dosage of TAM had obvious inhibitory effect on mammary hyperplasia under light microscope.Conclusion:Through light microscope and electronmicroscope observation,TAM especially 500?g and 667?g dosage proves to have obvious inhibitory effect on mammary hyperplasia induced by estradiol and progesterone.